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Corticosterone Injection Impairs Follicular Development, Ovulation and Steroidogenesis Capacity in Mice Ovary
SIMPLE SUMMARY: Researchers have hitherto established hundreds of animal stress models. However, these models have some limitations due to the complexity in operation and large differences between individual animals. In particular, there are few stress models that are specifically applied in mammali...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941055/ https://www.ncbi.nlm.nih.gov/pubmed/31795468 http://dx.doi.org/10.3390/ani9121047 |
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author | Wei, Yinghui Li, Weijian Meng, Xueqing Zhang, Liangliang Shen, Ming Liu, Honglin |
author_facet | Wei, Yinghui Li, Weijian Meng, Xueqing Zhang, Liangliang Shen, Ming Liu, Honglin |
author_sort | Wei, Yinghui |
collection | PubMed |
description | SIMPLE SUMMARY: Researchers have hitherto established hundreds of animal stress models. However, these models have some limitations due to the complexity in operation and large differences between individual animals. In particular, there are few stress models that are specifically applied in mammalian ovaries. In this study, using intraperitoneal injection of cortisol/corticosterone (CORT), we successfully established a stress model that acts on the ovarian function. Our data showed that CORT inhibits ovarian and follicular development and blocks ovulation. The establishment of this model might provide a living platform for studying ovarian stress in future research. ABSTRACT: The aim of this study is to establish an ovarian stress model, and to investigate the effects of stress on follicular development. Our data showed that continuous intraperitoneal injection of CORT successfully created a stressful environment in the ovary. To assess the effects of CORT on ovarian functions, 80 three-week-old ICR (Institute of Cancer Research) female mice were randomly divided into control group and treatment group. All mice were injected intraperitoneally with pregnant horse serum gonadotropin (PMSG). At the same time, the treatment group were injected with CORT (1 mg/mouse) at intervals of 8 h; while the control group was injected with same volume of methyl sulfoxide (DMSO). Blood, ovaries, or ovarian granulosa cell samples were collected at 24 h, 48 h, and 55 h after PMSG injection. The results showed that, compared with the control group, CORT-injected mice revealed a significant decrease in ovulation rates, ovarian weight, ovarian index, the number of secondary follicles and mature follicles, levels of estrogen and progesterone, and mRNA expression of steroid synthase-related genes. Collectively, our findings clearly demonstrated that CORT injection could represent an effective practice to simulate stresses that inhibit ovarian functions by reducing follicular development and ovulation. |
format | Online Article Text |
id | pubmed-6941055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69410552020-01-09 Corticosterone Injection Impairs Follicular Development, Ovulation and Steroidogenesis Capacity in Mice Ovary Wei, Yinghui Li, Weijian Meng, Xueqing Zhang, Liangliang Shen, Ming Liu, Honglin Animals (Basel) Article SIMPLE SUMMARY: Researchers have hitherto established hundreds of animal stress models. However, these models have some limitations due to the complexity in operation and large differences between individual animals. In particular, there are few stress models that are specifically applied in mammalian ovaries. In this study, using intraperitoneal injection of cortisol/corticosterone (CORT), we successfully established a stress model that acts on the ovarian function. Our data showed that CORT inhibits ovarian and follicular development and blocks ovulation. The establishment of this model might provide a living platform for studying ovarian stress in future research. ABSTRACT: The aim of this study is to establish an ovarian stress model, and to investigate the effects of stress on follicular development. Our data showed that continuous intraperitoneal injection of CORT successfully created a stressful environment in the ovary. To assess the effects of CORT on ovarian functions, 80 three-week-old ICR (Institute of Cancer Research) female mice were randomly divided into control group and treatment group. All mice were injected intraperitoneally with pregnant horse serum gonadotropin (PMSG). At the same time, the treatment group were injected with CORT (1 mg/mouse) at intervals of 8 h; while the control group was injected with same volume of methyl sulfoxide (DMSO). Blood, ovaries, or ovarian granulosa cell samples were collected at 24 h, 48 h, and 55 h after PMSG injection. The results showed that, compared with the control group, CORT-injected mice revealed a significant decrease in ovulation rates, ovarian weight, ovarian index, the number of secondary follicles and mature follicles, levels of estrogen and progesterone, and mRNA expression of steroid synthase-related genes. Collectively, our findings clearly demonstrated that CORT injection could represent an effective practice to simulate stresses that inhibit ovarian functions by reducing follicular development and ovulation. MDPI 2019-11-29 /pmc/articles/PMC6941055/ /pubmed/31795468 http://dx.doi.org/10.3390/ani9121047 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wei, Yinghui Li, Weijian Meng, Xueqing Zhang, Liangliang Shen, Ming Liu, Honglin Corticosterone Injection Impairs Follicular Development, Ovulation and Steroidogenesis Capacity in Mice Ovary |
title | Corticosterone Injection Impairs Follicular Development, Ovulation and Steroidogenesis Capacity in Mice Ovary |
title_full | Corticosterone Injection Impairs Follicular Development, Ovulation and Steroidogenesis Capacity in Mice Ovary |
title_fullStr | Corticosterone Injection Impairs Follicular Development, Ovulation and Steroidogenesis Capacity in Mice Ovary |
title_full_unstemmed | Corticosterone Injection Impairs Follicular Development, Ovulation and Steroidogenesis Capacity in Mice Ovary |
title_short | Corticosterone Injection Impairs Follicular Development, Ovulation and Steroidogenesis Capacity in Mice Ovary |
title_sort | corticosterone injection impairs follicular development, ovulation and steroidogenesis capacity in mice ovary |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941055/ https://www.ncbi.nlm.nih.gov/pubmed/31795468 http://dx.doi.org/10.3390/ani9121047 |
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