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Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle

Oviductal extracellular vesicles (oEVs) have been proposed as key modulators of gamete/embryo maternal interactions. The aim of this study was to examine the metabolite content of oEVs and its regulation across the estrous cycle in cattle. Oviductal EVs were isolated from bovine oviducts ipsilateral...

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Autores principales: Gatien, Julie, Mermillod, Pascal, Tsikis, Guillaume, Bernardi, Ophélie, Janati Idrissi, Sarah, Uzbekov, Rustem, Le Bourhis, Daniel, Salvetti, Pascal, Almiñana, Carmen, Saint-Dizier, Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941065/
https://www.ncbi.nlm.nih.gov/pubmed/31888194
http://dx.doi.org/10.3390/ijms20246339
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author Gatien, Julie
Mermillod, Pascal
Tsikis, Guillaume
Bernardi, Ophélie
Janati Idrissi, Sarah
Uzbekov, Rustem
Le Bourhis, Daniel
Salvetti, Pascal
Almiñana, Carmen
Saint-Dizier, Marie
author_facet Gatien, Julie
Mermillod, Pascal
Tsikis, Guillaume
Bernardi, Ophélie
Janati Idrissi, Sarah
Uzbekov, Rustem
Le Bourhis, Daniel
Salvetti, Pascal
Almiñana, Carmen
Saint-Dizier, Marie
author_sort Gatien, Julie
collection PubMed
description Oviductal extracellular vesicles (oEVs) have been proposed as key modulators of gamete/embryo maternal interactions. The aim of this study was to examine the metabolite content of oEVs and its regulation across the estrous cycle in cattle. Oviductal EVs were isolated from bovine oviducts ipsilateral and contralateral to ovulation at four stages of the estrous cycle (post-ovulatory stage, early and late luteal phases, and pre-ovulatory stage). The metabolomic profiling of EVs was performed by proton nuclear magnetic resonance spectroscopy (NMR). NMR identified 22 metabolites in oEVs, among which 15 were quantified. Lactate, myoinositol, and glycine were the most abundant metabolites throughout the estrous cycle. The side relative to ovulation had no effect on the oEVs’ metabolite concentrations. However, levels of glucose-1-phosphate and maltose were greatly affected by the cycle stage, showing up to 100-fold higher levels at the luteal phase than at the peri-ovulatory phases. In contrast, levels of methionine were significantly higher at peri-ovulatory phases than at the late-luteal phase. Quantitative enrichment analyses of oEV-metabolites across the cycle evidenced several significantly regulated metabolic pathways related to sucrose, glucose, and lactose metabolism. This study provides the first metabolomic characterization of oEVs, increasing our understanding of the potential role of oEVs in promoting fertilization and early embryo development.
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spelling pubmed-69410652020-01-09 Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle Gatien, Julie Mermillod, Pascal Tsikis, Guillaume Bernardi, Ophélie Janati Idrissi, Sarah Uzbekov, Rustem Le Bourhis, Daniel Salvetti, Pascal Almiñana, Carmen Saint-Dizier, Marie Int J Mol Sci Article Oviductal extracellular vesicles (oEVs) have been proposed as key modulators of gamete/embryo maternal interactions. The aim of this study was to examine the metabolite content of oEVs and its regulation across the estrous cycle in cattle. Oviductal EVs were isolated from bovine oviducts ipsilateral and contralateral to ovulation at four stages of the estrous cycle (post-ovulatory stage, early and late luteal phases, and pre-ovulatory stage). The metabolomic profiling of EVs was performed by proton nuclear magnetic resonance spectroscopy (NMR). NMR identified 22 metabolites in oEVs, among which 15 were quantified. Lactate, myoinositol, and glycine were the most abundant metabolites throughout the estrous cycle. The side relative to ovulation had no effect on the oEVs’ metabolite concentrations. However, levels of glucose-1-phosphate and maltose were greatly affected by the cycle stage, showing up to 100-fold higher levels at the luteal phase than at the peri-ovulatory phases. In contrast, levels of methionine were significantly higher at peri-ovulatory phases than at the late-luteal phase. Quantitative enrichment analyses of oEV-metabolites across the cycle evidenced several significantly regulated metabolic pathways related to sucrose, glucose, and lactose metabolism. This study provides the first metabolomic characterization of oEVs, increasing our understanding of the potential role of oEVs in promoting fertilization and early embryo development. MDPI 2019-12-16 /pmc/articles/PMC6941065/ /pubmed/31888194 http://dx.doi.org/10.3390/ijms20246339 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gatien, Julie
Mermillod, Pascal
Tsikis, Guillaume
Bernardi, Ophélie
Janati Idrissi, Sarah
Uzbekov, Rustem
Le Bourhis, Daniel
Salvetti, Pascal
Almiñana, Carmen
Saint-Dizier, Marie
Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle
title Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle
title_full Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle
title_fullStr Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle
title_full_unstemmed Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle
title_short Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle
title_sort metabolomic profile of oviductal extracellular vesicles across the estrous cycle in cattle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941065/
https://www.ncbi.nlm.nih.gov/pubmed/31888194
http://dx.doi.org/10.3390/ijms20246339
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