Heat Treatment at an Early Age Has Effects on the Resistance to Chronic Heat Stress on Broilers

SIMPLE SUMMARY: In this study, we investigated early heat exposure to a chronic heat-stressed group, of which has effects on growth performance, liver-specific enzymes (GOT; glutamic oxalacetic transaminase, and GPT; glutamic pyruvate transaminase), neuro (dopamine and serotonin) and stress (corticosterone) hormones, and the expression of HSPs (heat shock proteins), HSFs (heat shock factors), and pro-inflammatory cytokines. Chronic heat stress and early heat exposed groups reduced body weight, feed intake, and GOT, GPT, serotonin in the serum, and the protein expression of HSPs in liver tissue. At the same time, HSP70, HSP27, TNF-α, IFNG, and IL-6 gene expression were up-regulated in the early heat exposed group. According to study results regarding the broilers, early heat exposure has no effects on growth performance, physiology, and HSP protein expressions. ABSTRACT: This study was conducted to investigate the effects of early heat conditioning on growth performance, liver-specific enzymes (GOT and GPT), neuro-hormones (dopamine and serotonin), stress hormones (corticosterone), and the expression of HSPs (heat shock proteins), HSFs (heat shock factors), and pro-inflammatory cytokines under chronic high temperature. Broilers were raised with commercial feed and supplied with water ad libitum under conventional temperature. We separated the broilers into three groups: the control without any heat exposure (C), chronic heat-stressed group (CH), and early and chronic heat-stressed group (HH). At 5 days of age, the HH group was exposed to high temperatures (40 °C for 24 h), while the remaining groups were raised at a standard temperature. Between days 6 and 20, all three groups were kept under optimal temperature. From 21 to 35 days, the two heat-stressed groups (CH and HH) were exposed to 35 °C. Groups exposed to high temperature (CH and HH) showed significantly lower body weight and feed intake compared to the control. GOT and GPT were lower expressed in the CH and HH groups than the control group. In addition, the protein expressions of HSPs were down-regulated by chronic heat stress (CH and HH groups). The gene expressions of HSP60 and HSF3 were significantly down-regulated in the CH and HH groups, while HSP70 and HSP27 genes were up-regulated only in the HH group compared with the control group. The expression of pro-inflammatory cytokine genes was significantly up-regulated in the HH group compared with the control and CH groups. Thus, exposure of early Heat stress (HS) to broilers may affect the inflammatory response; however, early heat exposure did not have a positive effect on chronic HS of liver enzymes and heat shock protein expression.