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Association Between Antiarrhythmic, Electrophysiological, and Antioxidative Effects of Melatonin in Ischemia/Reperfusion

Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion u...

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Autores principales: Sedova, Ksenia A., Bernikova, Olesya G., Cuprova, Julia I., Ivanova, Alexandra D., Kutaeva, Galina A., Pliss, Michael G., Lopatina, Ekaterina V., Vaykshnorayte, Marina A., Diez, Emiliano R., Azarov, Jan E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941092/
https://www.ncbi.nlm.nih.gov/pubmed/31847485
http://dx.doi.org/10.3390/ijms20246331
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author Sedova, Ksenia A.
Bernikova, Olesya G.
Cuprova, Julia I.
Ivanova, Alexandra D.
Kutaeva, Galina A.
Pliss, Michael G.
Lopatina, Ekaterina V.
Vaykshnorayte, Marina A.
Diez, Emiliano R.
Azarov, Jan E.
author_facet Sedova, Ksenia A.
Bernikova, Olesya G.
Cuprova, Julia I.
Ivanova, Alexandra D.
Kutaeva, Galina A.
Pliss, Michael G.
Lopatina, Ekaterina V.
Vaykshnorayte, Marina A.
Diez, Emiliano R.
Azarov, Jan E.
author_sort Sedova, Ksenia A.
collection PubMed
description Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion under melatonin treatment. Melatonin was given to 28 rats (10 mg/kg/day, orally, for 7 days) and 13 animals received placebo. In the anesthetized animals, coronary occlusion was induced for 5 min followed by reperfusion with recording of unipolar electrograms from ventricular epicardium with a 64-lead array. Effects of melatonin on transmembrane potentials were studied in ventricular preparations of 7 rats in normal and “ischemic” conditions. Melatonin treatment was associated with lower VT/VF incidence at reperfusion, shorter baseline activation times (ATs), and activation-repolarization intervals and more complete recovery of repolarization times (RTs) at reperfusion (less baseline-reperfusion difference, ΔRT) (p < 0.05). Superoxide dismutase (SOD) activity was higher in the treated animals and associated with ΔRT (p = 0.001), whereas VT/VF incidence was associated with baseline ATs (p = 0.020). In vitro, melatonin led to a more complete restoration of action potential durations and resting membrane potentials at reoxygenation (p < 0.05). Thus, the antioxidative properties of melatonin were associated with its influence on repolarization duration, whereas the melatonin-related antiarrhythmic effect was associated with its oxidative stress-independent action on ventricular activation.
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spelling pubmed-69410922020-01-09 Association Between Antiarrhythmic, Electrophysiological, and Antioxidative Effects of Melatonin in Ischemia/Reperfusion Sedova, Ksenia A. Bernikova, Olesya G. Cuprova, Julia I. Ivanova, Alexandra D. Kutaeva, Galina A. Pliss, Michael G. Lopatina, Ekaterina V. Vaykshnorayte, Marina A. Diez, Emiliano R. Azarov, Jan E. Int J Mol Sci Article Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion under melatonin treatment. Melatonin was given to 28 rats (10 mg/kg/day, orally, for 7 days) and 13 animals received placebo. In the anesthetized animals, coronary occlusion was induced for 5 min followed by reperfusion with recording of unipolar electrograms from ventricular epicardium with a 64-lead array. Effects of melatonin on transmembrane potentials were studied in ventricular preparations of 7 rats in normal and “ischemic” conditions. Melatonin treatment was associated with lower VT/VF incidence at reperfusion, shorter baseline activation times (ATs), and activation-repolarization intervals and more complete recovery of repolarization times (RTs) at reperfusion (less baseline-reperfusion difference, ΔRT) (p < 0.05). Superoxide dismutase (SOD) activity was higher in the treated animals and associated with ΔRT (p = 0.001), whereas VT/VF incidence was associated with baseline ATs (p = 0.020). In vitro, melatonin led to a more complete restoration of action potential durations and resting membrane potentials at reoxygenation (p < 0.05). Thus, the antioxidative properties of melatonin were associated with its influence on repolarization duration, whereas the melatonin-related antiarrhythmic effect was associated with its oxidative stress-independent action on ventricular activation. MDPI 2019-12-15 /pmc/articles/PMC6941092/ /pubmed/31847485 http://dx.doi.org/10.3390/ijms20246331 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sedova, Ksenia A.
Bernikova, Olesya G.
Cuprova, Julia I.
Ivanova, Alexandra D.
Kutaeva, Galina A.
Pliss, Michael G.
Lopatina, Ekaterina V.
Vaykshnorayte, Marina A.
Diez, Emiliano R.
Azarov, Jan E.
Association Between Antiarrhythmic, Electrophysiological, and Antioxidative Effects of Melatonin in Ischemia/Reperfusion
title Association Between Antiarrhythmic, Electrophysiological, and Antioxidative Effects of Melatonin in Ischemia/Reperfusion
title_full Association Between Antiarrhythmic, Electrophysiological, and Antioxidative Effects of Melatonin in Ischemia/Reperfusion
title_fullStr Association Between Antiarrhythmic, Electrophysiological, and Antioxidative Effects of Melatonin in Ischemia/Reperfusion
title_full_unstemmed Association Between Antiarrhythmic, Electrophysiological, and Antioxidative Effects of Melatonin in Ischemia/Reperfusion
title_short Association Between Antiarrhythmic, Electrophysiological, and Antioxidative Effects of Melatonin in Ischemia/Reperfusion
title_sort association between antiarrhythmic, electrophysiological, and antioxidative effects of melatonin in ischemia/reperfusion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941092/
https://www.ncbi.nlm.nih.gov/pubmed/31847485
http://dx.doi.org/10.3390/ijms20246331
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