Cargando…

Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate in Ganoderma Lucidum

Ganoderma lucidum is widely recognized as a medicinal basidiomycete. It was previously reported that the plant hormone methyl jasmonate (MeJA) could induce the biosynthesis of ganoderic acids (GAs), which are the main active ingredients of G. lucidum. However, the regulatory mechanism is still uncle...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Ai-Liang, Liu, Yong-Nan, Liu, Rui, Ren, Ang, Ma, Hong-Yu, Shu, Lie-Bo, Shi, Liang, Zhu, Jing, Zhao, Ming-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941157/
https://www.ncbi.nlm.nih.gov/pubmed/31817230
http://dx.doi.org/10.3390/ijms20246116
_version_ 1783484495878422528
author Jiang, Ai-Liang
Liu, Yong-Nan
Liu, Rui
Ren, Ang
Ma, Hong-Yu
Shu, Lie-Bo
Shi, Liang
Zhu, Jing
Zhao, Ming-Wen
author_facet Jiang, Ai-Liang
Liu, Yong-Nan
Liu, Rui
Ren, Ang
Ma, Hong-Yu
Shu, Lie-Bo
Shi, Liang
Zhu, Jing
Zhao, Ming-Wen
author_sort Jiang, Ai-Liang
collection PubMed
description Ganoderma lucidum is widely recognized as a medicinal basidiomycete. It was previously reported that the plant hormone methyl jasmonate (MeJA) could induce the biosynthesis of ganoderic acids (GAs), which are the main active ingredients of G. lucidum. However, the regulatory mechanism is still unclear. In this study, integrated proteomics and metabolomics were employed on G. lucidum to globally identify differences in proteins and metabolites under MeJA treatment for 15 min (M15) and 24 h (M24). Our study successfully identified 209 differential abundance proteins (DAPs) in M15 and 202 DAPs in M24. We also identified 154 metabolites by GC–MS and 70 metabolites by LC–MS in M24 that are involved in several metabolic pathways. With an in-depth analysis, we found some DAPs and metabolites that are involved in the oxidoreduction process, secondary metabolism, energy metabolism, transcriptional and translational regulation, and protein synthesis. In particular, our results reveal that MeJA treatment leads to metabolic rearrangement that inhibited the normal glucose metabolism, energy supply, and protein synthesis of cells but promoted secondary metabolites, including GAs. In conclusion, our proteomics and metabolomics data further confirm the promoting effect of MeJA on the biosynthesis of GAs in G. lucidum and will provide a valuable resource for further investigation of the molecular mechanisms of MeJA signal response and GA biosynthesis in G. lucidum and other related species.
format Online
Article
Text
id pubmed-6941157
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69411572020-01-09 Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate in Ganoderma Lucidum Jiang, Ai-Liang Liu, Yong-Nan Liu, Rui Ren, Ang Ma, Hong-Yu Shu, Lie-Bo Shi, Liang Zhu, Jing Zhao, Ming-Wen Int J Mol Sci Article Ganoderma lucidum is widely recognized as a medicinal basidiomycete. It was previously reported that the plant hormone methyl jasmonate (MeJA) could induce the biosynthesis of ganoderic acids (GAs), which are the main active ingredients of G. lucidum. However, the regulatory mechanism is still unclear. In this study, integrated proteomics and metabolomics were employed on G. lucidum to globally identify differences in proteins and metabolites under MeJA treatment for 15 min (M15) and 24 h (M24). Our study successfully identified 209 differential abundance proteins (DAPs) in M15 and 202 DAPs in M24. We also identified 154 metabolites by GC–MS and 70 metabolites by LC–MS in M24 that are involved in several metabolic pathways. With an in-depth analysis, we found some DAPs and metabolites that are involved in the oxidoreduction process, secondary metabolism, energy metabolism, transcriptional and translational regulation, and protein synthesis. In particular, our results reveal that MeJA treatment leads to metabolic rearrangement that inhibited the normal glucose metabolism, energy supply, and protein synthesis of cells but promoted secondary metabolites, including GAs. In conclusion, our proteomics and metabolomics data further confirm the promoting effect of MeJA on the biosynthesis of GAs in G. lucidum and will provide a valuable resource for further investigation of the molecular mechanisms of MeJA signal response and GA biosynthesis in G. lucidum and other related species. MDPI 2019-12-04 /pmc/articles/PMC6941157/ /pubmed/31817230 http://dx.doi.org/10.3390/ijms20246116 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jiang, Ai-Liang
Liu, Yong-Nan
Liu, Rui
Ren, Ang
Ma, Hong-Yu
Shu, Lie-Bo
Shi, Liang
Zhu, Jing
Zhao, Ming-Wen
Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate in Ganoderma Lucidum
title Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate in Ganoderma Lucidum
title_full Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate in Ganoderma Lucidum
title_fullStr Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate in Ganoderma Lucidum
title_full_unstemmed Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate in Ganoderma Lucidum
title_short Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate in Ganoderma Lucidum
title_sort integrated proteomics and metabolomics analysis provides insights into ganoderic acid biosynthesis in response to methyl jasmonate in ganoderma lucidum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941157/
https://www.ncbi.nlm.nih.gov/pubmed/31817230
http://dx.doi.org/10.3390/ijms20246116
work_keys_str_mv AT jiangailiang integratedproteomicsandmetabolomicsanalysisprovidesinsightsintoganodericacidbiosynthesisinresponsetomethyljasmonateinganodermalucidum
AT liuyongnan integratedproteomicsandmetabolomicsanalysisprovidesinsightsintoganodericacidbiosynthesisinresponsetomethyljasmonateinganodermalucidum
AT liurui integratedproteomicsandmetabolomicsanalysisprovidesinsightsintoganodericacidbiosynthesisinresponsetomethyljasmonateinganodermalucidum
AT renang integratedproteomicsandmetabolomicsanalysisprovidesinsightsintoganodericacidbiosynthesisinresponsetomethyljasmonateinganodermalucidum
AT mahongyu integratedproteomicsandmetabolomicsanalysisprovidesinsightsintoganodericacidbiosynthesisinresponsetomethyljasmonateinganodermalucidum
AT shuliebo integratedproteomicsandmetabolomicsanalysisprovidesinsightsintoganodericacidbiosynthesisinresponsetomethyljasmonateinganodermalucidum
AT shiliang integratedproteomicsandmetabolomicsanalysisprovidesinsightsintoganodericacidbiosynthesisinresponsetomethyljasmonateinganodermalucidum
AT zhujing integratedproteomicsandmetabolomicsanalysisprovidesinsightsintoganodericacidbiosynthesisinresponsetomethyljasmonateinganodermalucidum
AT zhaomingwen integratedproteomicsandmetabolomicsanalysisprovidesinsightsintoganodericacidbiosynthesisinresponsetomethyljasmonateinganodermalucidum