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Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization
[Image: see text] Desorption/ionization induced by neutral clusters (DINeC) was employed for mass spectrometry (MS) of oligopeptides and lipids after separation by means of thin-layer chromatography (TLC). Clear and fragmentation-free spectra were obtained from the TLC plates without any further sam...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941192/ https://www.ncbi.nlm.nih.gov/pubmed/31909324 http://dx.doi.org/10.1021/acsomega.9b03060 |
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author | Heep, Julian Tuchecker, Paul H. K. Gebhardt, Christoph R. Dürr, Michael |
author_facet | Heep, Julian Tuchecker, Paul H. K. Gebhardt, Christoph R. Dürr, Michael |
author_sort | Heep, Julian |
collection | PubMed |
description | [Image: see text] Desorption/ionization induced by neutral clusters (DINeC) was employed for mass spectrometry (MS) of oligopeptides and lipids after separation by means of thin-layer chromatography (TLC). Clear and fragmentation-free spectra were obtained from the TLC plates without any further sample treatment. Mass-resolved chromatograms were deduced when scanning the TLC plates with the cluster beam along the direction of solvent movement. Using vancomycin and noncovalently bound complexes, the soft nature of DINeC was demonstrated also when used in combination with TLC. As a test application, TLC and DINeC–MS were employed to separate and detect different phospholipids obtained from egg yolk. |
format | Online Article Text |
id | pubmed-6941192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-69411922020-01-06 Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization Heep, Julian Tuchecker, Paul H. K. Gebhardt, Christoph R. Dürr, Michael ACS Omega [Image: see text] Desorption/ionization induced by neutral clusters (DINeC) was employed for mass spectrometry (MS) of oligopeptides and lipids after separation by means of thin-layer chromatography (TLC). Clear and fragmentation-free spectra were obtained from the TLC plates without any further sample treatment. Mass-resolved chromatograms were deduced when scanning the TLC plates with the cluster beam along the direction of solvent movement. Using vancomycin and noncovalently bound complexes, the soft nature of DINeC was demonstrated also when used in combination with TLC. As a test application, TLC and DINeC–MS were employed to separate and detect different phospholipids obtained from egg yolk. American Chemical Society 2019-12-20 /pmc/articles/PMC6941192/ /pubmed/31909324 http://dx.doi.org/10.1021/acsomega.9b03060 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Heep, Julian Tuchecker, Paul H. K. Gebhardt, Christoph R. Dürr, Michael Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization |
title | Combination of
Thin-Layer Chromatography and Mass
Spectrometry Using Cluster-Induced Desorption/Ionization |
title_full | Combination of
Thin-Layer Chromatography and Mass
Spectrometry Using Cluster-Induced Desorption/Ionization |
title_fullStr | Combination of
Thin-Layer Chromatography and Mass
Spectrometry Using Cluster-Induced Desorption/Ionization |
title_full_unstemmed | Combination of
Thin-Layer Chromatography and Mass
Spectrometry Using Cluster-Induced Desorption/Ionization |
title_short | Combination of
Thin-Layer Chromatography and Mass
Spectrometry Using Cluster-Induced Desorption/Ionization |
title_sort | combination of
thin-layer chromatography and mass
spectrometry using cluster-induced desorption/ionization |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941192/ https://www.ncbi.nlm.nih.gov/pubmed/31909324 http://dx.doi.org/10.1021/acsomega.9b03060 |
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