Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization

[Image: see text] Desorption/ionization induced by neutral clusters (DINeC) was employed for mass spectrometry (MS) of oligopeptides and lipids after separation by means of thin-layer chromatography (TLC). Clear and fragmentation-free spectra were obtained from the TLC plates without any further sam...

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Autores principales: Heep, Julian, Tuchecker, Paul H. K., Gebhardt, Christoph R., Dürr, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941192/
https://www.ncbi.nlm.nih.gov/pubmed/31909324
http://dx.doi.org/10.1021/acsomega.9b03060
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author Heep, Julian
Tuchecker, Paul H. K.
Gebhardt, Christoph R.
Dürr, Michael
author_facet Heep, Julian
Tuchecker, Paul H. K.
Gebhardt, Christoph R.
Dürr, Michael
author_sort Heep, Julian
collection PubMed
description [Image: see text] Desorption/ionization induced by neutral clusters (DINeC) was employed for mass spectrometry (MS) of oligopeptides and lipids after separation by means of thin-layer chromatography (TLC). Clear and fragmentation-free spectra were obtained from the TLC plates without any further sample treatment. Mass-resolved chromatograms were deduced when scanning the TLC plates with the cluster beam along the direction of solvent movement. Using vancomycin and noncovalently bound complexes, the soft nature of DINeC was demonstrated also when used in combination with TLC. As a test application, TLC and DINeC–MS were employed to separate and detect different phospholipids obtained from egg yolk.
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spelling pubmed-69411922020-01-06 Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization Heep, Julian Tuchecker, Paul H. K. Gebhardt, Christoph R. Dürr, Michael ACS Omega [Image: see text] Desorption/ionization induced by neutral clusters (DINeC) was employed for mass spectrometry (MS) of oligopeptides and lipids after separation by means of thin-layer chromatography (TLC). Clear and fragmentation-free spectra were obtained from the TLC plates without any further sample treatment. Mass-resolved chromatograms were deduced when scanning the TLC plates with the cluster beam along the direction of solvent movement. Using vancomycin and noncovalently bound complexes, the soft nature of DINeC was demonstrated also when used in combination with TLC. As a test application, TLC and DINeC–MS were employed to separate and detect different phospholipids obtained from egg yolk. American Chemical Society 2019-12-20 /pmc/articles/PMC6941192/ /pubmed/31909324 http://dx.doi.org/10.1021/acsomega.9b03060 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Heep, Julian
Tuchecker, Paul H. K.
Gebhardt, Christoph R.
Dürr, Michael
Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization
title Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization
title_full Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization
title_fullStr Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization
title_full_unstemmed Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization
title_short Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization
title_sort combination of thin-layer chromatography and mass spectrometry using cluster-induced desorption/ionization
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941192/
https://www.ncbi.nlm.nih.gov/pubmed/31909324
http://dx.doi.org/10.1021/acsomega.9b03060
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