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Diagnostic value of plasma HSP90α levels for detection of hepatocellular carcinoma

BACKGROUND: Hepatocellular carcinoma (HCC) is a major health problem worldwide. However, the popular tumor marker, AFP, lacks sensitivity although its specificity is high. Tissue biopsy is an invasive operation and may increase the risk of needle-track metastases. Heat shock protein 90 (HSP90) is a...

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Autores principales: Wei, Wene, Liu, Mengshu, Ning, Shufang, Wei, Jing, Zhong, Jianhong, Li, Jilin, Cai, Zhengmin, Zhang, Litu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941289/
https://www.ncbi.nlm.nih.gov/pubmed/31898536
http://dx.doi.org/10.1186/s12885-019-6489-0
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author Wei, Wene
Liu, Mengshu
Ning, Shufang
Wei, Jing
Zhong, Jianhong
Li, Jilin
Cai, Zhengmin
Zhang, Litu
author_facet Wei, Wene
Liu, Mengshu
Ning, Shufang
Wei, Jing
Zhong, Jianhong
Li, Jilin
Cai, Zhengmin
Zhang, Litu
author_sort Wei, Wene
collection PubMed
description BACKGROUND: Hepatocellular carcinoma (HCC) is a major health problem worldwide. However, the popular tumor marker, AFP, lacks sensitivity although its specificity is high. Tissue biopsy is an invasive operation and may increase the risk of needle-track metastases. Heat shock protein 90 (HSP90) is a potential biomarker for tumor diagnosis and prognosis. This study aims to determine whether levels of plasma HSP90α in HCC patients can be used as a cost-effective and simple test for the initial diagnosis of the disease. METHODS: Plasma samples were collected from 659 HCC patients, 114 secondary hepatic carcinoma (SHC) patients, 28 hepatic hemangioma patients and 230 healthy donors. The levels of HSP90α were measured by ELISA. RESULTS: The levels of plasma HSP90α in HCC patients were significantly higher than in healthy donors and in patients with hepatic hemangioma or SHC (144.08 ± 4.98, 46.81 ± 1.11, 61.56 ± 8.20 and 111.96 ± 10.08 ng/mL, respectively; p < 0.05 in all cases). The levels were associated with age (p = 0.001), BCLC stage (p < 0.001), levels of AFP (p < 0.001), tumor size (p < 0.001), tumor number (p < 0.001), PVTT (p < 0.001), EHM (p < 0.001) and Child-Pugh stage in the HCC cohort. In addition, the levels of plasma HSP90α showed an upward trend along with the progression of the BCLC stage. ROC curve analysis showed that compared to AFP (AUC 0.922, 95%CI 0.902–0.938) or HSP90α (AUC 0.836, 95%CI 0.810–0.860), the combination of HSP90α and AFP (AUC0.943, 95%CI 0.925–0.957) significantly improved the diagnostic efficiency for HCC patients. CONCLUSION: The results suggest that plasma Hsp90 α levels can be used as an initial diagnosis for patients with HCC in both rural and cosmopolitan settings.
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spelling pubmed-69412892020-01-06 Diagnostic value of plasma HSP90α levels for detection of hepatocellular carcinoma Wei, Wene Liu, Mengshu Ning, Shufang Wei, Jing Zhong, Jianhong Li, Jilin Cai, Zhengmin Zhang, Litu BMC Cancer Research Article BACKGROUND: Hepatocellular carcinoma (HCC) is a major health problem worldwide. However, the popular tumor marker, AFP, lacks sensitivity although its specificity is high. Tissue biopsy is an invasive operation and may increase the risk of needle-track metastases. Heat shock protein 90 (HSP90) is a potential biomarker for tumor diagnosis and prognosis. This study aims to determine whether levels of plasma HSP90α in HCC patients can be used as a cost-effective and simple test for the initial diagnosis of the disease. METHODS: Plasma samples were collected from 659 HCC patients, 114 secondary hepatic carcinoma (SHC) patients, 28 hepatic hemangioma patients and 230 healthy donors. The levels of HSP90α were measured by ELISA. RESULTS: The levels of plasma HSP90α in HCC patients were significantly higher than in healthy donors and in patients with hepatic hemangioma or SHC (144.08 ± 4.98, 46.81 ± 1.11, 61.56 ± 8.20 and 111.96 ± 10.08 ng/mL, respectively; p < 0.05 in all cases). The levels were associated with age (p = 0.001), BCLC stage (p < 0.001), levels of AFP (p < 0.001), tumor size (p < 0.001), tumor number (p < 0.001), PVTT (p < 0.001), EHM (p < 0.001) and Child-Pugh stage in the HCC cohort. In addition, the levels of plasma HSP90α showed an upward trend along with the progression of the BCLC stage. ROC curve analysis showed that compared to AFP (AUC 0.922, 95%CI 0.902–0.938) or HSP90α (AUC 0.836, 95%CI 0.810–0.860), the combination of HSP90α and AFP (AUC0.943, 95%CI 0.925–0.957) significantly improved the diagnostic efficiency for HCC patients. CONCLUSION: The results suggest that plasma Hsp90 α levels can be used as an initial diagnosis for patients with HCC in both rural and cosmopolitan settings. BioMed Central 2020-01-02 /pmc/articles/PMC6941289/ /pubmed/31898536 http://dx.doi.org/10.1186/s12885-019-6489-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wei, Wene
Liu, Mengshu
Ning, Shufang
Wei, Jing
Zhong, Jianhong
Li, Jilin
Cai, Zhengmin
Zhang, Litu
Diagnostic value of plasma HSP90α levels for detection of hepatocellular carcinoma
title Diagnostic value of plasma HSP90α levels for detection of hepatocellular carcinoma
title_full Diagnostic value of plasma HSP90α levels for detection of hepatocellular carcinoma
title_fullStr Diagnostic value of plasma HSP90α levels for detection of hepatocellular carcinoma
title_full_unstemmed Diagnostic value of plasma HSP90α levels for detection of hepatocellular carcinoma
title_short Diagnostic value of plasma HSP90α levels for detection of hepatocellular carcinoma
title_sort diagnostic value of plasma hsp90α levels for detection of hepatocellular carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941289/
https://www.ncbi.nlm.nih.gov/pubmed/31898536
http://dx.doi.org/10.1186/s12885-019-6489-0
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