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Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system
Bioluminescent detection has become a powerful method that is used extensively in numerous areas in life science research. Given that fluorescence detection in plant cells is difficult owing to the autofluorescence of chlorophyll, the use of a luciferin–luciferase system should be effective in plant...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941820/ https://www.ncbi.nlm.nih.gov/pubmed/31899919 http://dx.doi.org/10.1371/journal.pone.0227477 |
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author | Furuhata, Yuichi Sakai, Ayako Murakami, Tomi Nagasaki, Akira Kato, Yoshio |
author_facet | Furuhata, Yuichi Sakai, Ayako Murakami, Tomi Nagasaki, Akira Kato, Yoshio |
author_sort | Furuhata, Yuichi |
collection | PubMed |
description | Bioluminescent detection has become a powerful method that is used extensively in numerous areas in life science research. Given that fluorescence detection in plant cells is difficult owing to the autofluorescence of chlorophyll, the use of a luciferin–luciferase system should be effective in plant biology. However, the suitable optical window for a luminescence system in plants remains unexplored. In this study, we sought to determine the optical window and optimal luciferase reporter system for terrestrial plant analyses using Arabidopsis thaliana as a model organism. We compared six different luciferase systems and found the green enhanced Nano-lantern (GeNL)–furimazine combination to be the optimal luciferase reporter. Spectral measurements of GeNL–furimazine showed that its luminescence peak falls within the range of optical transparency for chlorophyll and, therefore, enables greater penetration through a layer of cultured A. thaliana cells. Moreover, A. thaliana plants expressing GeNL with furimazine emitted strong luminescence, which could be detected even with the naked eye. Thus, the GeNL–furimazine combination should facilitate biological analyses of genes and cellular functions in A. thaliana and all other terrestrial plants. |
format | Online Article Text |
id | pubmed-6941820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-69418202020-01-10 Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system Furuhata, Yuichi Sakai, Ayako Murakami, Tomi Nagasaki, Akira Kato, Yoshio PLoS One Research Article Bioluminescent detection has become a powerful method that is used extensively in numerous areas in life science research. Given that fluorescence detection in plant cells is difficult owing to the autofluorescence of chlorophyll, the use of a luciferin–luciferase system should be effective in plant biology. However, the suitable optical window for a luminescence system in plants remains unexplored. In this study, we sought to determine the optical window and optimal luciferase reporter system for terrestrial plant analyses using Arabidopsis thaliana as a model organism. We compared six different luciferase systems and found the green enhanced Nano-lantern (GeNL)–furimazine combination to be the optimal luciferase reporter. Spectral measurements of GeNL–furimazine showed that its luminescence peak falls within the range of optical transparency for chlorophyll and, therefore, enables greater penetration through a layer of cultured A. thaliana cells. Moreover, A. thaliana plants expressing GeNL with furimazine emitted strong luminescence, which could be detected even with the naked eye. Thus, the GeNL–furimazine combination should facilitate biological analyses of genes and cellular functions in A. thaliana and all other terrestrial plants. Public Library of Science 2020-01-03 /pmc/articles/PMC6941820/ /pubmed/31899919 http://dx.doi.org/10.1371/journal.pone.0227477 Text en © 2020 Furuhata et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Furuhata, Yuichi Sakai, Ayako Murakami, Tomi Nagasaki, Akira Kato, Yoshio Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system |
title | Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system |
title_full | Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system |
title_fullStr | Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system |
title_full_unstemmed | Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system |
title_short | Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system |
title_sort | bioluminescent imaging of arabidopsis thaliana using an enhanced nano-lantern luminescence reporter system |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941820/ https://www.ncbi.nlm.nih.gov/pubmed/31899919 http://dx.doi.org/10.1371/journal.pone.0227477 |
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