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Identification of antigens presented by MHC for vaccines against tuberculosis
Mycobacterium tuberculosis (M.tb) is responsible for more deaths globally than any other pathogen. The only available vaccine, bacillus Calmette-Guérin (BCG), has variable efficacy throughout the world. A more effective vaccine is urgently needed. The immune response against tuberculosis relies, at...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941960/ https://www.ncbi.nlm.nih.gov/pubmed/31908851 http://dx.doi.org/10.1038/s41541-019-0148-y |
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author | Bettencourt, Paulo Müller, Julius Nicastri, Annalisa Cantillon, Daire Madhavan, Meera Charles, Philip D. Fotso, Carine B. Wittenberg, Rachel Bull, Naomi Pinpathomrat, Nawamin Waddell, Simon J. Stylianou, Elena Hill, Adrian V. S. Ternette, Nicola McShane, Helen |
author_facet | Bettencourt, Paulo Müller, Julius Nicastri, Annalisa Cantillon, Daire Madhavan, Meera Charles, Philip D. Fotso, Carine B. Wittenberg, Rachel Bull, Naomi Pinpathomrat, Nawamin Waddell, Simon J. Stylianou, Elena Hill, Adrian V. S. Ternette, Nicola McShane, Helen |
author_sort | Bettencourt, Paulo |
collection | PubMed |
description | Mycobacterium tuberculosis (M.tb) is responsible for more deaths globally than any other pathogen. The only available vaccine, bacillus Calmette-Guérin (BCG), has variable efficacy throughout the world. A more effective vaccine is urgently needed. The immune response against tuberculosis relies, at least in part, on CD4(+) T cells. Protective vaccines require the induction of antigen-specific CD4(+) T cells via mycobacterial peptides presented by MHC class-II in infected macrophages. In order to identify mycobacterial antigens bound to MHC, we have immunoprecipitated MHC class-I and class-II complexes from THP-1 macrophages infected with BCG, purified MHC class-I and MHC class-II peptides and analysed them by liquid chromatography tandem mass spectrometry. We have successfully identified 94 mycobacterial peptides presented by MHC-II and 43 presented by MHC-I, from 76 and 41 antigens, respectively. These antigens were found to be highly expressed in infected macrophages. Gene ontology analysis suggests most of these antigens are associated with membranes and involved in lipid biosynthesis and transport. The sequences of selected peptides were confirmed by spectral match validation and immunogenicity evaluated by IFN-gamma ELISpot against peripheral blood mononuclear cell from volunteers vaccinated with BCG, M.tb latently infected subjects or patients with tuberculosis disease. Three antigens were expressed in viral vectors, and evaluated as vaccine candidates alone or in combination in a murine aerosol M.tb challenge model. When delivered in combination, the three candidate vaccines conferred significant protection in the lungs and spleen compared with BCG alone, demonstrating proof-of-concept for this unbiased approach to identifying new candidate antigens. |
format | Online Article Text |
id | pubmed-6941960 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69419602020-01-06 Identification of antigens presented by MHC for vaccines against tuberculosis Bettencourt, Paulo Müller, Julius Nicastri, Annalisa Cantillon, Daire Madhavan, Meera Charles, Philip D. Fotso, Carine B. Wittenberg, Rachel Bull, Naomi Pinpathomrat, Nawamin Waddell, Simon J. Stylianou, Elena Hill, Adrian V. S. Ternette, Nicola McShane, Helen NPJ Vaccines Article Mycobacterium tuberculosis (M.tb) is responsible for more deaths globally than any other pathogen. The only available vaccine, bacillus Calmette-Guérin (BCG), has variable efficacy throughout the world. A more effective vaccine is urgently needed. The immune response against tuberculosis relies, at least in part, on CD4(+) T cells. Protective vaccines require the induction of antigen-specific CD4(+) T cells via mycobacterial peptides presented by MHC class-II in infected macrophages. In order to identify mycobacterial antigens bound to MHC, we have immunoprecipitated MHC class-I and class-II complexes from THP-1 macrophages infected with BCG, purified MHC class-I and MHC class-II peptides and analysed them by liquid chromatography tandem mass spectrometry. We have successfully identified 94 mycobacterial peptides presented by MHC-II and 43 presented by MHC-I, from 76 and 41 antigens, respectively. These antigens were found to be highly expressed in infected macrophages. Gene ontology analysis suggests most of these antigens are associated with membranes and involved in lipid biosynthesis and transport. The sequences of selected peptides were confirmed by spectral match validation and immunogenicity evaluated by IFN-gamma ELISpot against peripheral blood mononuclear cell from volunteers vaccinated with BCG, M.tb latently infected subjects or patients with tuberculosis disease. Three antigens were expressed in viral vectors, and evaluated as vaccine candidates alone or in combination in a murine aerosol M.tb challenge model. When delivered in combination, the three candidate vaccines conferred significant protection in the lungs and spleen compared with BCG alone, demonstrating proof-of-concept for this unbiased approach to identifying new candidate antigens. Nature Publishing Group UK 2020-01-03 /pmc/articles/PMC6941960/ /pubmed/31908851 http://dx.doi.org/10.1038/s41541-019-0148-y Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Bettencourt, Paulo Müller, Julius Nicastri, Annalisa Cantillon, Daire Madhavan, Meera Charles, Philip D. Fotso, Carine B. Wittenberg, Rachel Bull, Naomi Pinpathomrat, Nawamin Waddell, Simon J. Stylianou, Elena Hill, Adrian V. S. Ternette, Nicola McShane, Helen Identification of antigens presented by MHC for vaccines against tuberculosis |
title | Identification of antigens presented by MHC for vaccines against tuberculosis |
title_full | Identification of antigens presented by MHC for vaccines against tuberculosis |
title_fullStr | Identification of antigens presented by MHC for vaccines against tuberculosis |
title_full_unstemmed | Identification of antigens presented by MHC for vaccines against tuberculosis |
title_short | Identification of antigens presented by MHC for vaccines against tuberculosis |
title_sort | identification of antigens presented by mhc for vaccines against tuberculosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941960/ https://www.ncbi.nlm.nih.gov/pubmed/31908851 http://dx.doi.org/10.1038/s41541-019-0148-y |
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