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Two-hybrid screening of FAM13A protein partners in lung epithelial cells
OBJECTIVES: Family with sequence similarity 13 member A (FAM13A) genetic variants have been associated with several chronic respiratory diseases including chronic obstructive pulmonary disease (COPD), cystic fibrosis (CF), idiopathic pulmonary fibrosis (IPF) and lung cancer. The FAM13A protein inclu...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942259/ https://www.ncbi.nlm.nih.gov/pubmed/31900205 http://dx.doi.org/10.1186/s13104-019-4840-9 |
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author | Ruffin, Manon Thompson, Kristin E. Corvol, Harriet Guillot, Loic |
author_facet | Ruffin, Manon Thompson, Kristin E. Corvol, Harriet Guillot, Loic |
author_sort | Ruffin, Manon |
collection | PubMed |
description | OBJECTIVES: Family with sequence similarity 13 member A (FAM13A) genetic variants have been associated with several chronic respiratory diseases including chronic obstructive pulmonary disease (COPD), cystic fibrosis (CF), idiopathic pulmonary fibrosis (IPF) and lung cancer. The FAM13A protein includes a RhoGTPase activating protein (RhoGAP) domain known to participate in various cellular mechanisms including cell proliferation. While intensive genomic studies have been performed to reveal its involvement in lung diseases, the biological role of FAM13A protein is still not completely elucidated. RESULTS: We therefore performed a two-hybrid screening to identify protein partners of FAM13A using a human lung cancer cDNA library. We identified several protein partners with a high confidence score. Researchers in the field of chronic lung diseases may benefit from this two-hybrid screening data which may reveal new research pathways to decipher. |
format | Online Article Text |
id | pubmed-6942259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-69422592020-01-07 Two-hybrid screening of FAM13A protein partners in lung epithelial cells Ruffin, Manon Thompson, Kristin E. Corvol, Harriet Guillot, Loic BMC Res Notes Research Note OBJECTIVES: Family with sequence similarity 13 member A (FAM13A) genetic variants have been associated with several chronic respiratory diseases including chronic obstructive pulmonary disease (COPD), cystic fibrosis (CF), idiopathic pulmonary fibrosis (IPF) and lung cancer. The FAM13A protein includes a RhoGTPase activating protein (RhoGAP) domain known to participate in various cellular mechanisms including cell proliferation. While intensive genomic studies have been performed to reveal its involvement in lung diseases, the biological role of FAM13A protein is still not completely elucidated. RESULTS: We therefore performed a two-hybrid screening to identify protein partners of FAM13A using a human lung cancer cDNA library. We identified several protein partners with a high confidence score. Researchers in the field of chronic lung diseases may benefit from this two-hybrid screening data which may reveal new research pathways to decipher. BioMed Central 2020-01-03 /pmc/articles/PMC6942259/ /pubmed/31900205 http://dx.doi.org/10.1186/s13104-019-4840-9 Text en © The Author(s) 2019 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Note Ruffin, Manon Thompson, Kristin E. Corvol, Harriet Guillot, Loic Two-hybrid screening of FAM13A protein partners in lung epithelial cells |
title | Two-hybrid screening of FAM13A protein partners in lung epithelial cells |
title_full | Two-hybrid screening of FAM13A protein partners in lung epithelial cells |
title_fullStr | Two-hybrid screening of FAM13A protein partners in lung epithelial cells |
title_full_unstemmed | Two-hybrid screening of FAM13A protein partners in lung epithelial cells |
title_short | Two-hybrid screening of FAM13A protein partners in lung epithelial cells |
title_sort | two-hybrid screening of fam13a protein partners in lung epithelial cells |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942259/ https://www.ncbi.nlm.nih.gov/pubmed/31900205 http://dx.doi.org/10.1186/s13104-019-4840-9 |
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