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LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis
BACKGROUND: Long non-coding RNAs are important regulators in cancer cell tumorigenesis. We have demonstrated in a prior study that lncRNA FTX is dysregulated in gastric cancer (GC). In this study, we aim to report gastric cancer-related lncRNA FTX as a main regulator in GC development and progressio...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942532/ https://www.ncbi.nlm.nih.gov/pubmed/32021248 http://dx.doi.org/10.2147/OTT.S220998 |
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author | Li, Hongbo Yao, Guoliang Zhai, Jingming Hu, Dingwen Fan, Yonggang |
author_facet | Li, Hongbo Yao, Guoliang Zhai, Jingming Hu, Dingwen Fan, Yonggang |
author_sort | Li, Hongbo |
collection | PubMed |
description | BACKGROUND: Long non-coding RNAs are important regulators in cancer cell tumorigenesis. We have demonstrated in a prior study that lncRNA FTX is dysregulated in gastric cancer (GC). In this study, we aim to report gastric cancer-related lncRNA FTX as a main regulator in GC development and progression. METHODS: In vitro and in vivo assays of FTX alterations have been performed to reveal a complex integrated phenotype affecting cell growth, migration, and invasion. lncRNA FTX expression levels in gastric cancer cells and normal cells were measured by RT-PCR. Luciferase reporter assays, Western blotting, and many immune, microscopy technologies were utilized to investigate the expressions of FTX- related proteins and RNAs. The functional role of FTX in cell growth, migration, and invasion were observed in vitro and in vivo. RESULTS: We explored the underlying mechanisms of FTX in GC development, and the microRNAs’ relationship with FTX. We found that FTX promoted cell proliferation, migration, and invasion, as well as tumor growth, and this effect could latterly be attenuated by miR-144. ZFX attenuated the effects of FTX/miR-144 axis by sponging with miR-144. CONCLUSION: In summary, the above results support a model in which the FTX/miR-144/ZFX act as important effectors in GC tumorigenesis and progression, indicating new therapeutic methods in GC. |
format | Online Article Text |
id | pubmed-6942532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-69425322020-02-04 LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis Li, Hongbo Yao, Guoliang Zhai, Jingming Hu, Dingwen Fan, Yonggang Onco Targets Ther Original Research BACKGROUND: Long non-coding RNAs are important regulators in cancer cell tumorigenesis. We have demonstrated in a prior study that lncRNA FTX is dysregulated in gastric cancer (GC). In this study, we aim to report gastric cancer-related lncRNA FTX as a main regulator in GC development and progression. METHODS: In vitro and in vivo assays of FTX alterations have been performed to reveal a complex integrated phenotype affecting cell growth, migration, and invasion. lncRNA FTX expression levels in gastric cancer cells and normal cells were measured by RT-PCR. Luciferase reporter assays, Western blotting, and many immune, microscopy technologies were utilized to investigate the expressions of FTX- related proteins and RNAs. The functional role of FTX in cell growth, migration, and invasion were observed in vitro and in vivo. RESULTS: We explored the underlying mechanisms of FTX in GC development, and the microRNAs’ relationship with FTX. We found that FTX promoted cell proliferation, migration, and invasion, as well as tumor growth, and this effect could latterly be attenuated by miR-144. ZFX attenuated the effects of FTX/miR-144 axis by sponging with miR-144. CONCLUSION: In summary, the above results support a model in which the FTX/miR-144/ZFX act as important effectors in GC tumorigenesis and progression, indicating new therapeutic methods in GC. Dove 2019-12-31 /pmc/articles/PMC6942532/ /pubmed/32021248 http://dx.doi.org/10.2147/OTT.S220998 Text en © 2019 Li et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Li, Hongbo Yao, Guoliang Zhai, Jingming Hu, Dingwen Fan, Yonggang LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis |
title | LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis |
title_full | LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis |
title_fullStr | LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis |
title_full_unstemmed | LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis |
title_short | LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis |
title_sort | lncrna ftx promotes proliferation and invasion of gastric cancer via mir-144/zfx axis |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942532/ https://www.ncbi.nlm.nih.gov/pubmed/32021248 http://dx.doi.org/10.2147/OTT.S220998 |
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