Cargando…

LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis

BACKGROUND: Long non-coding RNAs are important regulators in cancer cell tumorigenesis. We have demonstrated in a prior study that lncRNA FTX is dysregulated in gastric cancer (GC). In this study, we aim to report gastric cancer-related lncRNA FTX as a main regulator in GC development and progressio...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Hongbo, Yao, Guoliang, Zhai, Jingming, Hu, Dingwen, Fan, Yonggang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942532/
https://www.ncbi.nlm.nih.gov/pubmed/32021248
http://dx.doi.org/10.2147/OTT.S220998
_version_ 1783484725629812736
author Li, Hongbo
Yao, Guoliang
Zhai, Jingming
Hu, Dingwen
Fan, Yonggang
author_facet Li, Hongbo
Yao, Guoliang
Zhai, Jingming
Hu, Dingwen
Fan, Yonggang
author_sort Li, Hongbo
collection PubMed
description BACKGROUND: Long non-coding RNAs are important regulators in cancer cell tumorigenesis. We have demonstrated in a prior study that lncRNA FTX is dysregulated in gastric cancer (GC). In this study, we aim to report gastric cancer-related lncRNA FTX as a main regulator in GC development and progression. METHODS: In vitro and in vivo assays of FTX alterations have been performed to reveal a complex integrated phenotype affecting cell growth, migration, and invasion. lncRNA FTX expression levels in gastric cancer cells and normal cells were measured by RT-PCR. Luciferase reporter assays, Western blotting, and many immune, microscopy technologies were utilized to investigate the expressions of FTX- related proteins and RNAs. The functional role of FTX in cell growth, migration, and invasion were observed in vitro and in vivo. RESULTS: We explored the underlying mechanisms of FTX in GC development, and the microRNAs’ relationship with FTX. We found that FTX promoted cell proliferation, migration, and invasion, as well as tumor growth, and this effect could latterly be attenuated by miR-144. ZFX attenuated the effects of FTX/miR-144 axis by sponging with miR-144. CONCLUSION: In summary, the above results support a model in which the FTX/miR-144/ZFX act as important effectors in GC tumorigenesis and progression, indicating new therapeutic methods in GC.
format Online
Article
Text
id pubmed-6942532
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-69425322020-02-04 LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis Li, Hongbo Yao, Guoliang Zhai, Jingming Hu, Dingwen Fan, Yonggang Onco Targets Ther Original Research BACKGROUND: Long non-coding RNAs are important regulators in cancer cell tumorigenesis. We have demonstrated in a prior study that lncRNA FTX is dysregulated in gastric cancer (GC). In this study, we aim to report gastric cancer-related lncRNA FTX as a main regulator in GC development and progression. METHODS: In vitro and in vivo assays of FTX alterations have been performed to reveal a complex integrated phenotype affecting cell growth, migration, and invasion. lncRNA FTX expression levels in gastric cancer cells and normal cells were measured by RT-PCR. Luciferase reporter assays, Western blotting, and many immune, microscopy technologies were utilized to investigate the expressions of FTX- related proteins and RNAs. The functional role of FTX in cell growth, migration, and invasion were observed in vitro and in vivo. RESULTS: We explored the underlying mechanisms of FTX in GC development, and the microRNAs’ relationship with FTX. We found that FTX promoted cell proliferation, migration, and invasion, as well as tumor growth, and this effect could latterly be attenuated by miR-144. ZFX attenuated the effects of FTX/miR-144 axis by sponging with miR-144. CONCLUSION: In summary, the above results support a model in which the FTX/miR-144/ZFX act as important effectors in GC tumorigenesis and progression, indicating new therapeutic methods in GC. Dove 2019-12-31 /pmc/articles/PMC6942532/ /pubmed/32021248 http://dx.doi.org/10.2147/OTT.S220998 Text en © 2019 Li et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Li, Hongbo
Yao, Guoliang
Zhai, Jingming
Hu, Dingwen
Fan, Yonggang
LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis
title LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis
title_full LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis
title_fullStr LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis
title_full_unstemmed LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis
title_short LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis
title_sort lncrna ftx promotes proliferation and invasion of gastric cancer via mir-144/zfx axis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942532/
https://www.ncbi.nlm.nih.gov/pubmed/32021248
http://dx.doi.org/10.2147/OTT.S220998
work_keys_str_mv AT lihongbo lncrnaftxpromotesproliferationandinvasionofgastriccancerviamir144zfxaxis
AT yaoguoliang lncrnaftxpromotesproliferationandinvasionofgastriccancerviamir144zfxaxis
AT zhaijingming lncrnaftxpromotesproliferationandinvasionofgastriccancerviamir144zfxaxis
AT hudingwen lncrnaftxpromotesproliferationandinvasionofgastriccancerviamir144zfxaxis
AT fanyonggang lncrnaftxpromotesproliferationandinvasionofgastriccancerviamir144zfxaxis