Cargando…
DUXAP8 a Pan-Cancer Prognostic Marker Involved in the Molecular Regulatory Mechanism in Hepatocellular Carcinoma: A Comprehensive Study Based on Data Mining, Bioinformatics, and in vitro Validation
BACKGROUND: Double homeobox A pseudogene 8 (DUXAP8) has been identified as a key regulator at the posttranscriptional level in various types of cancers. However, whether DUXAP8 has a role in hepatocellular carcinoma (HCC) progression remains to be determined. Here, we aimed to investigate the potent...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942538/ https://www.ncbi.nlm.nih.gov/pubmed/32021243 http://dx.doi.org/10.2147/OTT.S231750 |
_version_ | 1783484727108304896 |
---|---|
author | Yue, Chaosen Liang, Chaojie Li, Pengyang Yan, Lijun Zhang, Dongxin Xu, Yingchen Wei, Zhigang Wu, Jixiang |
author_facet | Yue, Chaosen Liang, Chaojie Li, Pengyang Yan, Lijun Zhang, Dongxin Xu, Yingchen Wei, Zhigang Wu, Jixiang |
author_sort | Yue, Chaosen |
collection | PubMed |
description | BACKGROUND: Double homeobox A pseudogene 8 (DUXAP8) has been identified as a key regulator at the posttranscriptional level in various types of cancers. However, whether DUXAP8 has a role in hepatocellular carcinoma (HCC) progression remains to be determined. Here, we aimed to investigate the potential clinical value of DUXAP8 as a pan-cancer marker, and its role in HCC development through an integrated analysis strategy and in vitro experimental validation. METHODS: Comprehensive analysis was performed using data mined from public databases to evaluate the expression patterns and clinical value of DUXAP8 in human pan-cancers. Bioinformatics analysis was performed to investigate the potential biological functions of DUXAP8 in HCC based on TCGA database. Real-time qPCR analysis was used to examine the expression levels of DUXAP8 in HCC tissue samples and cell lines. DUXAP8-siRNA was used to silence DUXAP8 in the Hep-G2 cell line to examine the role of DUXAP8 in HCC cell proliferation and invasion. RESULTS: DUXAP8 was significantly upregulated in various types of human cancers and could serve as a potential pan-cancer diagnostic and prognostic biomarker. Bioinformatics analysis suggested that DUXAP8 might be involved in the regulation of the biological processes of HCC cell cycle, cell division and cell proliferation. Additionally, downregulation of DUXAP8 inhibited HCC cell proliferation and invasion in vitro. CONCLUSION: This study revealed that DUXAP8 may serve as a potential pan-cancer prognostic and diagnostic marker in humans. In addition, DUXAP8 promoted HCC cell proliferation and invasion, suggesting that it may represent a novel therapeutic target for HCC. |
format | Online Article Text |
id | pubmed-6942538 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-69425382020-02-04 DUXAP8 a Pan-Cancer Prognostic Marker Involved in the Molecular Regulatory Mechanism in Hepatocellular Carcinoma: A Comprehensive Study Based on Data Mining, Bioinformatics, and in vitro Validation Yue, Chaosen Liang, Chaojie Li, Pengyang Yan, Lijun Zhang, Dongxin Xu, Yingchen Wei, Zhigang Wu, Jixiang Onco Targets Ther Original Research BACKGROUND: Double homeobox A pseudogene 8 (DUXAP8) has been identified as a key regulator at the posttranscriptional level in various types of cancers. However, whether DUXAP8 has a role in hepatocellular carcinoma (HCC) progression remains to be determined. Here, we aimed to investigate the potential clinical value of DUXAP8 as a pan-cancer marker, and its role in HCC development through an integrated analysis strategy and in vitro experimental validation. METHODS: Comprehensive analysis was performed using data mined from public databases to evaluate the expression patterns and clinical value of DUXAP8 in human pan-cancers. Bioinformatics analysis was performed to investigate the potential biological functions of DUXAP8 in HCC based on TCGA database. Real-time qPCR analysis was used to examine the expression levels of DUXAP8 in HCC tissue samples and cell lines. DUXAP8-siRNA was used to silence DUXAP8 in the Hep-G2 cell line to examine the role of DUXAP8 in HCC cell proliferation and invasion. RESULTS: DUXAP8 was significantly upregulated in various types of human cancers and could serve as a potential pan-cancer diagnostic and prognostic biomarker. Bioinformatics analysis suggested that DUXAP8 might be involved in the regulation of the biological processes of HCC cell cycle, cell division and cell proliferation. Additionally, downregulation of DUXAP8 inhibited HCC cell proliferation and invasion in vitro. CONCLUSION: This study revealed that DUXAP8 may serve as a potential pan-cancer prognostic and diagnostic marker in humans. In addition, DUXAP8 promoted HCC cell proliferation and invasion, suggesting that it may represent a novel therapeutic target for HCC. Dove 2019-12-31 /pmc/articles/PMC6942538/ /pubmed/32021243 http://dx.doi.org/10.2147/OTT.S231750 Text en © 2019 Yue et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Yue, Chaosen Liang, Chaojie Li, Pengyang Yan, Lijun Zhang, Dongxin Xu, Yingchen Wei, Zhigang Wu, Jixiang DUXAP8 a Pan-Cancer Prognostic Marker Involved in the Molecular Regulatory Mechanism in Hepatocellular Carcinoma: A Comprehensive Study Based on Data Mining, Bioinformatics, and in vitro Validation |
title | DUXAP8 a Pan-Cancer Prognostic Marker Involved in the Molecular Regulatory Mechanism in Hepatocellular Carcinoma: A Comprehensive Study Based on Data Mining, Bioinformatics, and in vitro Validation |
title_full | DUXAP8 a Pan-Cancer Prognostic Marker Involved in the Molecular Regulatory Mechanism in Hepatocellular Carcinoma: A Comprehensive Study Based on Data Mining, Bioinformatics, and in vitro Validation |
title_fullStr | DUXAP8 a Pan-Cancer Prognostic Marker Involved in the Molecular Regulatory Mechanism in Hepatocellular Carcinoma: A Comprehensive Study Based on Data Mining, Bioinformatics, and in vitro Validation |
title_full_unstemmed | DUXAP8 a Pan-Cancer Prognostic Marker Involved in the Molecular Regulatory Mechanism in Hepatocellular Carcinoma: A Comprehensive Study Based on Data Mining, Bioinformatics, and in vitro Validation |
title_short | DUXAP8 a Pan-Cancer Prognostic Marker Involved in the Molecular Regulatory Mechanism in Hepatocellular Carcinoma: A Comprehensive Study Based on Data Mining, Bioinformatics, and in vitro Validation |
title_sort | duxap8 a pan-cancer prognostic marker involved in the molecular regulatory mechanism in hepatocellular carcinoma: a comprehensive study based on data mining, bioinformatics, and in vitro validation |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942538/ https://www.ncbi.nlm.nih.gov/pubmed/32021243 http://dx.doi.org/10.2147/OTT.S231750 |
work_keys_str_mv | AT yuechaosen duxap8apancancerprognosticmarkerinvolvedinthemolecularregulatorymechanisminhepatocellularcarcinomaacomprehensivestudybasedondataminingbioinformaticsandinvitrovalidation AT liangchaojie duxap8apancancerprognosticmarkerinvolvedinthemolecularregulatorymechanisminhepatocellularcarcinomaacomprehensivestudybasedondataminingbioinformaticsandinvitrovalidation AT lipengyang duxap8apancancerprognosticmarkerinvolvedinthemolecularregulatorymechanisminhepatocellularcarcinomaacomprehensivestudybasedondataminingbioinformaticsandinvitrovalidation AT yanlijun duxap8apancancerprognosticmarkerinvolvedinthemolecularregulatorymechanisminhepatocellularcarcinomaacomprehensivestudybasedondataminingbioinformaticsandinvitrovalidation AT zhangdongxin duxap8apancancerprognosticmarkerinvolvedinthemolecularregulatorymechanisminhepatocellularcarcinomaacomprehensivestudybasedondataminingbioinformaticsandinvitrovalidation AT xuyingchen duxap8apancancerprognosticmarkerinvolvedinthemolecularregulatorymechanisminhepatocellularcarcinomaacomprehensivestudybasedondataminingbioinformaticsandinvitrovalidation AT weizhigang duxap8apancancerprognosticmarkerinvolvedinthemolecularregulatorymechanisminhepatocellularcarcinomaacomprehensivestudybasedondataminingbioinformaticsandinvitrovalidation AT wujixiang duxap8apancancerprognosticmarkerinvolvedinthemolecularregulatorymechanisminhepatocellularcarcinomaacomprehensivestudybasedondataminingbioinformaticsandinvitrovalidation |