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A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming

The Sleeping Beauty (SB) transposon is an advanced tool for genetic engineering and a useful model to investigate cut-and-paste DNA transposition in vertebrate cells. Here, we identify novel SB transposase mutants that display efficient and canonical excision but practically unmeasurable genomic re-...

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Detalles Bibliográficos
Autores principales:	Kesselring, Lisa, Miskey, Csaba, Zuliani, Cecilia, Querques, Irma, Kapitonov, Vladimir, Laukó, Andrea, Fehér, Anita, Palazzo, Antonio, Diem, Tanja, Lustig, Janna, Sebe, Attila, Wang, Yongming, Dinnyés, András, Izsvák, Zsuzsanna, Barabas, Orsolya, Ivics, Zoltán
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943129/ https://www.ncbi.nlm.nih.gov/pubmed/31777924 http://dx.doi.org/10.1093/nar/gkz1119

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