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Insecticidal Activity and Insecticidal Mechanism of Total Saponins from Camellia oleifera
Chemical pesticides are commonly used during the cultivation of agricultural products to control pests and diseases. Excessive use of traditional pesticides can cause environmental and human health risks. There are ongoing searches for new plant-derived pesticides to reduce the use of chemical pesti...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943515/ https://www.ncbi.nlm.nih.gov/pubmed/31835551 http://dx.doi.org/10.3390/molecules24244518 |
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author | Cui, Chuanjian Yang, Yunqin Zhao, Tianyu Zou, Kangkang Peng, Chuanyi Cai, Huimei Wan, Xiaochun Hou, Ruyan |
author_facet | Cui, Chuanjian Yang, Yunqin Zhao, Tianyu Zou, Kangkang Peng, Chuanyi Cai, Huimei Wan, Xiaochun Hou, Ruyan |
author_sort | Cui, Chuanjian |
collection | PubMed |
description | Chemical pesticides are commonly used during the cultivation of agricultural products to control pests and diseases. Excessive use of traditional pesticides can cause environmental and human health risks. There are ongoing searches for new plant-derived pesticides to reduce the use of chemical pesticides. In this study, tea saponin extracts of different purities were extracted from Camellia oleifera seeds using AB-8 macroporous resin and gradient elution with ethanol. The insecticidal effects of the tea saponin extracts were evaluated by contact toxicity tests and stomach toxicity tests using the lepidopteran pest of tea plantation, Ectropis obliqua. The total saponins extracted using 70% ethanol showed strong contact toxicity (LC(50) = 8.459 mg/L) and stomach toxicity (LC(50) = 22.395 mg/L). In-depth mechanistic studies demonstrated that tea saponins can disrupt the waxy layer of the epidermis, causing serious loss of water, and can penetrate the inside of the intestine of E. obliqua. After consumption of the tea saponins, the intestinal villi were shortened and the cavities of the intestinal wall were disrupted, which resulted in larval death. This study highlights the potential of tea saponins as a natural, plant-derived pesticide for the management of plant pests. |
format | Online Article Text |
id | pubmed-6943515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69435152020-01-10 Insecticidal Activity and Insecticidal Mechanism of Total Saponins from Camellia oleifera Cui, Chuanjian Yang, Yunqin Zhao, Tianyu Zou, Kangkang Peng, Chuanyi Cai, Huimei Wan, Xiaochun Hou, Ruyan Molecules Article Chemical pesticides are commonly used during the cultivation of agricultural products to control pests and diseases. Excessive use of traditional pesticides can cause environmental and human health risks. There are ongoing searches for new plant-derived pesticides to reduce the use of chemical pesticides. In this study, tea saponin extracts of different purities were extracted from Camellia oleifera seeds using AB-8 macroporous resin and gradient elution with ethanol. The insecticidal effects of the tea saponin extracts were evaluated by contact toxicity tests and stomach toxicity tests using the lepidopteran pest of tea plantation, Ectropis obliqua. The total saponins extracted using 70% ethanol showed strong contact toxicity (LC(50) = 8.459 mg/L) and stomach toxicity (LC(50) = 22.395 mg/L). In-depth mechanistic studies demonstrated that tea saponins can disrupt the waxy layer of the epidermis, causing serious loss of water, and can penetrate the inside of the intestine of E. obliqua. After consumption of the tea saponins, the intestinal villi were shortened and the cavities of the intestinal wall were disrupted, which resulted in larval death. This study highlights the potential of tea saponins as a natural, plant-derived pesticide for the management of plant pests. MDPI 2019-12-10 /pmc/articles/PMC6943515/ /pubmed/31835551 http://dx.doi.org/10.3390/molecules24244518 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cui, Chuanjian Yang, Yunqin Zhao, Tianyu Zou, Kangkang Peng, Chuanyi Cai, Huimei Wan, Xiaochun Hou, Ruyan Insecticidal Activity and Insecticidal Mechanism of Total Saponins from Camellia oleifera |
title | Insecticidal Activity and Insecticidal Mechanism of Total Saponins from Camellia oleifera |
title_full | Insecticidal Activity and Insecticidal Mechanism of Total Saponins from Camellia oleifera |
title_fullStr | Insecticidal Activity and Insecticidal Mechanism of Total Saponins from Camellia oleifera |
title_full_unstemmed | Insecticidal Activity and Insecticidal Mechanism of Total Saponins from Camellia oleifera |
title_short | Insecticidal Activity and Insecticidal Mechanism of Total Saponins from Camellia oleifera |
title_sort | insecticidal activity and insecticidal mechanism of total saponins from camellia oleifera |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943515/ https://www.ncbi.nlm.nih.gov/pubmed/31835551 http://dx.doi.org/10.3390/molecules24244518 |
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