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Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses
The root of Gentiana straminea Maxim. (Gentianaceae), is officially listed as “Qin-Jiao” in the Chinese Pharmacopoeia for the treatment of rheumatic arthritis, icteric hepatitis, constipation, pain, and hypertension. To establish the geographical origin traceability in G. straminea, its chemical pro...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943584/ https://www.ncbi.nlm.nih.gov/pubmed/31817679 http://dx.doi.org/10.3390/molecules24244478 |
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author | Pan, Zheng Xiong, Feng Chen, Yi-Long Wan, Guo-Guo Zhang, Yi Chen, Zhi-Wei Cao, Wen-Fu Zhou, Guo-Ying |
author_facet | Pan, Zheng Xiong, Feng Chen, Yi-Long Wan, Guo-Guo Zhang, Yi Chen, Zhi-Wei Cao, Wen-Fu Zhou, Guo-Ying |
author_sort | Pan, Zheng |
collection | PubMed |
description | The root of Gentiana straminea Maxim. (Gentianaceae), is officially listed as “Qin-Jiao” in the Chinese Pharmacopoeia for the treatment of rheumatic arthritis, icteric hepatitis, constipation, pain, and hypertension. To establish the geographical origin traceability in G. straminea, its chemical profiles were determined by a UPLC-Q exactive mass spectrometer, from which 43 compounds were identified by comparing retention times and mass spectrometry. Meanwhile, a pair of isomers (loganin and secologanol) was identified by mass spectrometry based on their fragmentation pathway. A total of 42 samples from difference habitats were determined by an UPLC-Q exactive mass spectrometer and the data were assayed with multivariate statistical analysis. Eight characteristic compounds were identified to determine the geographical origin of the herb. To estimate the key characteristic markers associated with pharmacological function, the inhibiting activities of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced macrophages were examined. This finding is crucial in realizing the determination of botanical origin and evaluating the quality of G. straminea. |
format | Online Article Text |
id | pubmed-6943584 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69435842020-01-10 Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses Pan, Zheng Xiong, Feng Chen, Yi-Long Wan, Guo-Guo Zhang, Yi Chen, Zhi-Wei Cao, Wen-Fu Zhou, Guo-Ying Molecules Article The root of Gentiana straminea Maxim. (Gentianaceae), is officially listed as “Qin-Jiao” in the Chinese Pharmacopoeia for the treatment of rheumatic arthritis, icteric hepatitis, constipation, pain, and hypertension. To establish the geographical origin traceability in G. straminea, its chemical profiles were determined by a UPLC-Q exactive mass spectrometer, from which 43 compounds were identified by comparing retention times and mass spectrometry. Meanwhile, a pair of isomers (loganin and secologanol) was identified by mass spectrometry based on their fragmentation pathway. A total of 42 samples from difference habitats were determined by an UPLC-Q exactive mass spectrometer and the data were assayed with multivariate statistical analysis. Eight characteristic compounds were identified to determine the geographical origin of the herb. To estimate the key characteristic markers associated with pharmacological function, the inhibiting activities of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced macrophages were examined. This finding is crucial in realizing the determination of botanical origin and evaluating the quality of G. straminea. MDPI 2019-12-06 /pmc/articles/PMC6943584/ /pubmed/31817679 http://dx.doi.org/10.3390/molecules24244478 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pan, Zheng Xiong, Feng Chen, Yi-Long Wan, Guo-Guo Zhang, Yi Chen, Zhi-Wei Cao, Wen-Fu Zhou, Guo-Ying Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses |
title | Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses |
title_full | Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses |
title_fullStr | Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses |
title_full_unstemmed | Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses |
title_short | Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses |
title_sort | traceability of geographical origin in gentiana straminea by uplc-q exactive mass and multivariate analyses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943584/ https://www.ncbi.nlm.nih.gov/pubmed/31817679 http://dx.doi.org/10.3390/molecules24244478 |
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