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Extraction and Determination of Polar Bioactive Compounds from Alfalfa (Medicago sativa L.) Using Supercritical Techniques

The aim of this research was to select parameters for supercritical extraction with CO(2) of Medicago sativa L., considered as functional food, in quarter-technical plant, providing the highest concentration of bioactive polar constituents and simultaneously maintaining the highest efficiency of the...

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Autores principales: Wrona, Olga, Rafińska, Katarzyna, Walczak-Skierska, Justyna, Możeński, Cezary, Buszewski, Bogusław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943590/
https://www.ncbi.nlm.nih.gov/pubmed/31888264
http://dx.doi.org/10.3390/molecules24244608
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author Wrona, Olga
Rafińska, Katarzyna
Walczak-Skierska, Justyna
Możeński, Cezary
Buszewski, Bogusław
author_facet Wrona, Olga
Rafińska, Katarzyna
Walczak-Skierska, Justyna
Możeński, Cezary
Buszewski, Bogusław
author_sort Wrona, Olga
collection PubMed
description The aim of this research was to select parameters for supercritical extraction with CO(2) of Medicago sativa L., considered as functional food, in quarter-technical plant, providing the highest concentration of bioactive polar constituents and simultaneously maintaining the highest efficiency of the process. For the purpose of optimization, mathematical statistics was used. Qualitative analysis of products was performed with supercritical fluid chromatography (SFC). The SFC analysis revealed a proper separation of flavonoids and phenolics acids for dedicated TFC and TPC optimal parameters. The obtained results have proved that it is a possibility to extract polar compounds with non-polar solvent under higher values of pressure and temperature and to enrich product with desired group of bioactive compounds with proper optimization. The proposed extraction technique allows to obtain on an industrial scale, using an environmentally friendly solvent, a preparation rich in biologically active nutrients that can be implemented in the cosmetics, pharmaceutical and food industries.
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spelling pubmed-69435902020-01-10 Extraction and Determination of Polar Bioactive Compounds from Alfalfa (Medicago sativa L.) Using Supercritical Techniques Wrona, Olga Rafińska, Katarzyna Walczak-Skierska, Justyna Możeński, Cezary Buszewski, Bogusław Molecules Article The aim of this research was to select parameters for supercritical extraction with CO(2) of Medicago sativa L., considered as functional food, in quarter-technical plant, providing the highest concentration of bioactive polar constituents and simultaneously maintaining the highest efficiency of the process. For the purpose of optimization, mathematical statistics was used. Qualitative analysis of products was performed with supercritical fluid chromatography (SFC). The SFC analysis revealed a proper separation of flavonoids and phenolics acids for dedicated TFC and TPC optimal parameters. The obtained results have proved that it is a possibility to extract polar compounds with non-polar solvent under higher values of pressure and temperature and to enrich product with desired group of bioactive compounds with proper optimization. The proposed extraction technique allows to obtain on an industrial scale, using an environmentally friendly solvent, a preparation rich in biologically active nutrients that can be implemented in the cosmetics, pharmaceutical and food industries. MDPI 2019-12-16 /pmc/articles/PMC6943590/ /pubmed/31888264 http://dx.doi.org/10.3390/molecules24244608 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wrona, Olga
Rafińska, Katarzyna
Walczak-Skierska, Justyna
Możeński, Cezary
Buszewski, Bogusław
Extraction and Determination of Polar Bioactive Compounds from Alfalfa (Medicago sativa L.) Using Supercritical Techniques
title Extraction and Determination of Polar Bioactive Compounds from Alfalfa (Medicago sativa L.) Using Supercritical Techniques
title_full Extraction and Determination of Polar Bioactive Compounds from Alfalfa (Medicago sativa L.) Using Supercritical Techniques
title_fullStr Extraction and Determination of Polar Bioactive Compounds from Alfalfa (Medicago sativa L.) Using Supercritical Techniques
title_full_unstemmed Extraction and Determination of Polar Bioactive Compounds from Alfalfa (Medicago sativa L.) Using Supercritical Techniques
title_short Extraction and Determination of Polar Bioactive Compounds from Alfalfa (Medicago sativa L.) Using Supercritical Techniques
title_sort extraction and determination of polar bioactive compounds from alfalfa (medicago sativa l.) using supercritical techniques
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943590/
https://www.ncbi.nlm.nih.gov/pubmed/31888264
http://dx.doi.org/10.3390/molecules24244608
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