Cargando…

Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm

Biofilm-associated infections are difficult to manage or treat as biofilms or biofilm-embedded bacteria are difficult to eradicate. Antimicrobial peptides have gained increasing attention as a possible alternative to conventional drugs to combat drug-resistant microorganisms because they inhibit the...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Seong-Cheol, Lee, Min-Young, Kim, Jin-Young, Kim, Hyeonseok, Jung, Myunghwan, Shin, Min-Kyoung, Lee, Woo-Kon, Cheong, Gang-Won, Lee, Jung Ro, Jang, Mi-Kyeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943720/
https://www.ncbi.nlm.nih.gov/pubmed/31842508
http://dx.doi.org/10.3390/molecules24244560
_version_ 1783484938642784256
author Park, Seong-Cheol
Lee, Min-Young
Kim, Jin-Young
Kim, Hyeonseok
Jung, Myunghwan
Shin, Min-Kyoung
Lee, Woo-Kon
Cheong, Gang-Won
Lee, Jung Ro
Jang, Mi-Kyeong
author_facet Park, Seong-Cheol
Lee, Min-Young
Kim, Jin-Young
Kim, Hyeonseok
Jung, Myunghwan
Shin, Min-Kyoung
Lee, Woo-Kon
Cheong, Gang-Won
Lee, Jung Ro
Jang, Mi-Kyeong
author_sort Park, Seong-Cheol
collection PubMed
description Biofilm-associated infections are difficult to manage or treat as biofilms or biofilm-embedded bacteria are difficult to eradicate. Antimicrobial peptides have gained increasing attention as a possible alternative to conventional drugs to combat drug-resistant microorganisms because they inhibit the growth of planktonic bacteria by disrupting the cytoplasmic membrane. The current study investigated the effects of synthetic peptides (PS1-2, PS1-5, and PS1-6) and conventional antibiotics on the growth, biofilm formation, and biofilm reduction of drug-resistant Pseudomonas aeruginosa and Staphylococcus aureus. The effects of PS1-2, PS1-5, and PS1-6 were also tested in vivo using a mouse model. All peptides inhibited planktonic cell growth and biofilm formation in a dose-dependent manner. They also reduced preformed biofilm masses by removing the carbohydrates, extracellular DNA, and lipids that comprised extracellular polymeric substances (EPSs) but did not affect proteins. In vivo, PS1-2 showed the greatest efficacy against preformed biofilms with no cytotoxicity. Our findings indicate that the PS1-2 peptide has potential as a next-generation therapeutic drug to overcome multidrug resistance and to regulate inflammatory response in biofilm-associated infections.
format Online
Article
Text
id pubmed-6943720
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69437202020-01-10 Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm Park, Seong-Cheol Lee, Min-Young Kim, Jin-Young Kim, Hyeonseok Jung, Myunghwan Shin, Min-Kyoung Lee, Woo-Kon Cheong, Gang-Won Lee, Jung Ro Jang, Mi-Kyeong Molecules Article Biofilm-associated infections are difficult to manage or treat as biofilms or biofilm-embedded bacteria are difficult to eradicate. Antimicrobial peptides have gained increasing attention as a possible alternative to conventional drugs to combat drug-resistant microorganisms because they inhibit the growth of planktonic bacteria by disrupting the cytoplasmic membrane. The current study investigated the effects of synthetic peptides (PS1-2, PS1-5, and PS1-6) and conventional antibiotics on the growth, biofilm formation, and biofilm reduction of drug-resistant Pseudomonas aeruginosa and Staphylococcus aureus. The effects of PS1-2, PS1-5, and PS1-6 were also tested in vivo using a mouse model. All peptides inhibited planktonic cell growth and biofilm formation in a dose-dependent manner. They also reduced preformed biofilm masses by removing the carbohydrates, extracellular DNA, and lipids that comprised extracellular polymeric substances (EPSs) but did not affect proteins. In vivo, PS1-2 showed the greatest efficacy against preformed biofilms with no cytotoxicity. Our findings indicate that the PS1-2 peptide has potential as a next-generation therapeutic drug to overcome multidrug resistance and to regulate inflammatory response in biofilm-associated infections. MDPI 2019-12-12 /pmc/articles/PMC6943720/ /pubmed/31842508 http://dx.doi.org/10.3390/molecules24244560 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Seong-Cheol
Lee, Min-Young
Kim, Jin-Young
Kim, Hyeonseok
Jung, Myunghwan
Shin, Min-Kyoung
Lee, Woo-Kon
Cheong, Gang-Won
Lee, Jung Ro
Jang, Mi-Kyeong
Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm
title Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm
title_full Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm
title_fullStr Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm
title_full_unstemmed Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm
title_short Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm
title_sort anti-biofilm effects of synthetic antimicrobial peptides against drug-resistant pseudomonas aeruginosa and staphylococcus aureus planktonic cells and biofilm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943720/
https://www.ncbi.nlm.nih.gov/pubmed/31842508
http://dx.doi.org/10.3390/molecules24244560
work_keys_str_mv AT parkseongcheol antibiofilmeffectsofsyntheticantimicrobialpeptidesagainstdrugresistantpseudomonasaeruginosaandstaphylococcusaureusplanktoniccellsandbiofilm
AT leeminyoung antibiofilmeffectsofsyntheticantimicrobialpeptidesagainstdrugresistantpseudomonasaeruginosaandstaphylococcusaureusplanktoniccellsandbiofilm
AT kimjinyoung antibiofilmeffectsofsyntheticantimicrobialpeptidesagainstdrugresistantpseudomonasaeruginosaandstaphylococcusaureusplanktoniccellsandbiofilm
AT kimhyeonseok antibiofilmeffectsofsyntheticantimicrobialpeptidesagainstdrugresistantpseudomonasaeruginosaandstaphylococcusaureusplanktoniccellsandbiofilm
AT jungmyunghwan antibiofilmeffectsofsyntheticantimicrobialpeptidesagainstdrugresistantpseudomonasaeruginosaandstaphylococcusaureusplanktoniccellsandbiofilm
AT shinminkyoung antibiofilmeffectsofsyntheticantimicrobialpeptidesagainstdrugresistantpseudomonasaeruginosaandstaphylococcusaureusplanktoniccellsandbiofilm
AT leewookon antibiofilmeffectsofsyntheticantimicrobialpeptidesagainstdrugresistantpseudomonasaeruginosaandstaphylococcusaureusplanktoniccellsandbiofilm
AT cheonggangwon antibiofilmeffectsofsyntheticantimicrobialpeptidesagainstdrugresistantpseudomonasaeruginosaandstaphylococcusaureusplanktoniccellsandbiofilm
AT leejungro antibiofilmeffectsofsyntheticantimicrobialpeptidesagainstdrugresistantpseudomonasaeruginosaandstaphylococcusaureusplanktoniccellsandbiofilm
AT jangmikyeong antibiofilmeffectsofsyntheticantimicrobialpeptidesagainstdrugresistantpseudomonasaeruginosaandstaphylococcusaureusplanktoniccellsandbiofilm