Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity

The fungal strain Alternaria alternata JS0515 was isolated from Vitex rotundifolia (beach vitex). Twelve secondary metabolites, including one new altenusin derivative (1), were isolated. The isolated metabolites included seven known altenusin derivatives (2–8), two isochromanones (9, 10), one peryle...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Changyeol, Li, Wei, Bang, Sunghee, Lee, Sun Joo, Kang, Nam-young, Kim, Soonok, Kim, Tae In, Go, Younghoon, Shim, Sang Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943735/
https://www.ncbi.nlm.nih.gov/pubmed/31817301
http://dx.doi.org/10.3390/molecules24244450
_version_ 1783484942160756736
author Lee, Changyeol
Li, Wei
Bang, Sunghee
Lee, Sun Joo
Kang, Nam-young
Kim, Soonok
Kim, Tae In
Go, Younghoon
Shim, Sang Hee
author_facet Lee, Changyeol
Li, Wei
Bang, Sunghee
Lee, Sun Joo
Kang, Nam-young
Kim, Soonok
Kim, Tae In
Go, Younghoon
Shim, Sang Hee
author_sort Lee, Changyeol
collection PubMed
description The fungal strain Alternaria alternata JS0515 was isolated from Vitex rotundifolia (beach vitex). Twelve secondary metabolites, including one new altenusin derivative (1), were isolated. The isolated metabolites included seven known altenusin derivatives (2–8), two isochromanones (9, 10), one perylenequinone (11), and one benzocycloalkanone (12). Their structures were determined via 1D and 2D nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), and computational electronic circular dichroism (ECD) analysis. Compounds 3 and 11 increased pyruvate dehydrogenase (PDH) activity in AD-293 human embryonic kidney cells and significantly inhibited PDH phosphorylation. The IC(50) values of 3 and 11 were 32.58 and 27.82 μM, respectively.
format Online
Article
Text
id pubmed-6943735
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69437352020-01-10 Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity Lee, Changyeol Li, Wei Bang, Sunghee Lee, Sun Joo Kang, Nam-young Kim, Soonok Kim, Tae In Go, Younghoon Shim, Sang Hee Molecules Communication The fungal strain Alternaria alternata JS0515 was isolated from Vitex rotundifolia (beach vitex). Twelve secondary metabolites, including one new altenusin derivative (1), were isolated. The isolated metabolites included seven known altenusin derivatives (2–8), two isochromanones (9, 10), one perylenequinone (11), and one benzocycloalkanone (12). Their structures were determined via 1D and 2D nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), and computational electronic circular dichroism (ECD) analysis. Compounds 3 and 11 increased pyruvate dehydrogenase (PDH) activity in AD-293 human embryonic kidney cells and significantly inhibited PDH phosphorylation. The IC(50) values of 3 and 11 were 32.58 and 27.82 μM, respectively. MDPI 2019-12-04 /pmc/articles/PMC6943735/ /pubmed/31817301 http://dx.doi.org/10.3390/molecules24244450 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Lee, Changyeol
Li, Wei
Bang, Sunghee
Lee, Sun Joo
Kang, Nam-young
Kim, Soonok
Kim, Tae In
Go, Younghoon
Shim, Sang Hee
Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity
title Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity
title_full Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity
title_fullStr Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity
title_full_unstemmed Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity
title_short Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity
title_sort secondary metabolites of the endophytic fungus alternaria alternata js0515 isolated from vitex rotundifolia and their effects on pyruvate dehydrogenase activity
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943735/
https://www.ncbi.nlm.nih.gov/pubmed/31817301
http://dx.doi.org/10.3390/molecules24244450
work_keys_str_mv AT leechangyeol secondarymetabolitesoftheendophyticfungusalternariaalternatajs0515isolatedfromvitexrotundifoliaandtheireffectsonpyruvatedehydrogenaseactivity
AT liwei secondarymetabolitesoftheendophyticfungusalternariaalternatajs0515isolatedfromvitexrotundifoliaandtheireffectsonpyruvatedehydrogenaseactivity
AT bangsunghee secondarymetabolitesoftheendophyticfungusalternariaalternatajs0515isolatedfromvitexrotundifoliaandtheireffectsonpyruvatedehydrogenaseactivity
AT leesunjoo secondarymetabolitesoftheendophyticfungusalternariaalternatajs0515isolatedfromvitexrotundifoliaandtheireffectsonpyruvatedehydrogenaseactivity
AT kangnamyoung secondarymetabolitesoftheendophyticfungusalternariaalternatajs0515isolatedfromvitexrotundifoliaandtheireffectsonpyruvatedehydrogenaseactivity
AT kimsoonok secondarymetabolitesoftheendophyticfungusalternariaalternatajs0515isolatedfromvitexrotundifoliaandtheireffectsonpyruvatedehydrogenaseactivity
AT kimtaein secondarymetabolitesoftheendophyticfungusalternariaalternatajs0515isolatedfromvitexrotundifoliaandtheireffectsonpyruvatedehydrogenaseactivity
AT goyounghoon secondarymetabolitesoftheendophyticfungusalternariaalternatajs0515isolatedfromvitexrotundifoliaandtheireffectsonpyruvatedehydrogenaseactivity
AT shimsanghee secondarymetabolitesoftheendophyticfungusalternariaalternatajs0515isolatedfromvitexrotundifoliaandtheireffectsonpyruvatedehydrogenaseactivity

Ejemplares similares