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Rad51 paralogs and the risk of unselected breast cancer: A case-control study

A case-control study was conducted in which we evaluated the association between genetic variability of DNA repair proteins belonging to the Rad51 family and breast cancer (BrC) risk. In the study, 132 female BrC cases and 189 healthy control females were genotyped for a total of 14 common single nu...

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Autores principales: Grešner, Peter, Jabłońska, Ewa, Gromadzińska, Jolanta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944361/
https://www.ncbi.nlm.nih.gov/pubmed/31905201
http://dx.doi.org/10.1371/journal.pone.0226976
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author Grešner, Peter
Jabłońska, Ewa
Gromadzińska, Jolanta
author_facet Grešner, Peter
Jabłońska, Ewa
Gromadzińska, Jolanta
author_sort Grešner, Peter
collection PubMed
description A case-control study was conducted in which we evaluated the association between genetic variability of DNA repair proteins belonging to the Rad51 family and breast cancer (BrC) risk. In the study, 132 female BrC cases and 189 healthy control females were genotyped for a total of 14 common single nucleotide polymorphisms (SNPs) within Rad51 and Xrcc3. Moreover, our previously reported Rad51C genetic data were involved to explore the nonlinear interactions among SNPs within the three genes and effect of such interactions on BrC risk. The rare rs5030789 genotype ((-4601)AA) in Rad51 was found to significantly decrease the BrC risk (OR = 0.5, 95% CI: 0.3–1.0, p<0.05). An interaction between this SNP, rs2619679 and rs2928140 (both in Rad51), was found to result in a two three-locus genotypes (-4719)AA/(-4601)AA/(2972)CG and (-4719)AT/(-4601)GA/(2972)CC, both of which were found to increase the risk of BrC (OR = 8.4, 95% CI: 1.8–38.6, p<0.0001), instead. Furthermore, rare Rad51 rs1801320 ((135)CC) and heterozygous Xrcc3 rs3212057 ((10343)GA) genotypes were found to respectively increase (OR = 10.6, 95% CI: 1.9–198, p<0.02) and decrease (OR = 0.0, 95% CI: 0.0-NA, p<0.05) the risk of BrC. Associations between these SNPs and BrC risk were further supported by outcomes of employed machine learning analyses. In Xrcc3, the (4541)A/(9685)A haplotype was found to be significantly associated with reduced BrC risk (OR = 0.5; 95% CI: 0.3–0.9; p<0.05). Concluding, our study indicates a complex role of SNPs within Rad51 (especially rs5030789) and Xrcc3 in BrC, although their significance with respect to the disease needs to be further clarified.
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spelling pubmed-69443612020-01-17 Rad51 paralogs and the risk of unselected breast cancer: A case-control study Grešner, Peter Jabłońska, Ewa Gromadzińska, Jolanta PLoS One Research Article A case-control study was conducted in which we evaluated the association between genetic variability of DNA repair proteins belonging to the Rad51 family and breast cancer (BrC) risk. In the study, 132 female BrC cases and 189 healthy control females were genotyped for a total of 14 common single nucleotide polymorphisms (SNPs) within Rad51 and Xrcc3. Moreover, our previously reported Rad51C genetic data were involved to explore the nonlinear interactions among SNPs within the three genes and effect of such interactions on BrC risk. The rare rs5030789 genotype ((-4601)AA) in Rad51 was found to significantly decrease the BrC risk (OR = 0.5, 95% CI: 0.3–1.0, p<0.05). An interaction between this SNP, rs2619679 and rs2928140 (both in Rad51), was found to result in a two three-locus genotypes (-4719)AA/(-4601)AA/(2972)CG and (-4719)AT/(-4601)GA/(2972)CC, both of which were found to increase the risk of BrC (OR = 8.4, 95% CI: 1.8–38.6, p<0.0001), instead. Furthermore, rare Rad51 rs1801320 ((135)CC) and heterozygous Xrcc3 rs3212057 ((10343)GA) genotypes were found to respectively increase (OR = 10.6, 95% CI: 1.9–198, p<0.02) and decrease (OR = 0.0, 95% CI: 0.0-NA, p<0.05) the risk of BrC. Associations between these SNPs and BrC risk were further supported by outcomes of employed machine learning analyses. In Xrcc3, the (4541)A/(9685)A haplotype was found to be significantly associated with reduced BrC risk (OR = 0.5; 95% CI: 0.3–0.9; p<0.05). Concluding, our study indicates a complex role of SNPs within Rad51 (especially rs5030789) and Xrcc3 in BrC, although their significance with respect to the disease needs to be further clarified. Public Library of Science 2020-01-06 /pmc/articles/PMC6944361/ /pubmed/31905201 http://dx.doi.org/10.1371/journal.pone.0226976 Text en © 2020 Grešner et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Grešner, Peter
Jabłońska, Ewa
Gromadzińska, Jolanta
Rad51 paralogs and the risk of unselected breast cancer: A case-control study
title Rad51 paralogs and the risk of unselected breast cancer: A case-control study
title_full Rad51 paralogs and the risk of unselected breast cancer: A case-control study
title_fullStr Rad51 paralogs and the risk of unselected breast cancer: A case-control study
title_full_unstemmed Rad51 paralogs and the risk of unselected breast cancer: A case-control study
title_short Rad51 paralogs and the risk of unselected breast cancer: A case-control study
title_sort rad51 paralogs and the risk of unselected breast cancer: a case-control study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944361/
https://www.ncbi.nlm.nih.gov/pubmed/31905201
http://dx.doi.org/10.1371/journal.pone.0226976
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