Detailed analysis at a single‐cell level of cells undergoing pancreatic differentiation

Pancreatic cells derived from pluripotent stem cells using the stem cell‐derived‐β (SC‐β) differentiation protocol are composed of four main cell types, namely β‐like cells (SC‐β‐cells), α‐like cells, enterochromaffin‐like cells and non‐endocrine cells. Single‐cell dissociation and reaggregation depleted non‐endocrine cells and improved β‐cell function. Veres et al. succeeded in efficiently purifying SC‐β by magnetic cell sorting using CD49a antibody and reaggregation, so that the final SC‐β‐cell ratio increased to 80%.[Image: see text]