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Chemogenetic interactions in human cancer cells

Chemogenetic profiling enables the identification of genes that enhance or suppress the phenotypic effect of chemical compounds. Using this approach in cancer therapies could improve our ability to predict the response of specific tumor genotypes to chemotherapeutic agents, thus accelerating the dev...

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Detalles Bibliográficos
Autores principales: Colic, Medina, Hart, Traver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945272/
https://www.ncbi.nlm.nih.gov/pubmed/31921397
http://dx.doi.org/10.1016/j.csbj.2019.09.006
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author Colic, Medina
Hart, Traver
author_facet Colic, Medina
Hart, Traver
author_sort Colic, Medina
collection PubMed
description Chemogenetic profiling enables the identification of genes that enhance or suppress the phenotypic effect of chemical compounds. Using this approach in cancer therapies could improve our ability to predict the response of specific tumor genotypes to chemotherapeutic agents, thus accelerating the development of personalized drug therapy. In the not so distant past, this strategy was only applied in model organisms because there was no feasible technology to thoroughly exploit desired genetic mutations and their impact on drug efficacy in human cells. Today, with the advent of CRISPR gene-editing technology and its application to pooled library screens in mammalian cells, chemogenetic screens are performed directly in human cell lines with high sensitivity and specificity. Chemogenetic profiling provides insights into drug mechanism-of-action, genetic vulnerabilities, and resistance mechanisms, all of which will help to accurately deliver the right drug to the right target in the right patient while minimizing side effects.
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spelling pubmed-69452722020-01-09 Chemogenetic interactions in human cancer cells Colic, Medina Hart, Traver Comput Struct Biotechnol J Review Article Chemogenetic profiling enables the identification of genes that enhance or suppress the phenotypic effect of chemical compounds. Using this approach in cancer therapies could improve our ability to predict the response of specific tumor genotypes to chemotherapeutic agents, thus accelerating the development of personalized drug therapy. In the not so distant past, this strategy was only applied in model organisms because there was no feasible technology to thoroughly exploit desired genetic mutations and their impact on drug efficacy in human cells. Today, with the advent of CRISPR gene-editing technology and its application to pooled library screens in mammalian cells, chemogenetic screens are performed directly in human cell lines with high sensitivity and specificity. Chemogenetic profiling provides insights into drug mechanism-of-action, genetic vulnerabilities, and resistance mechanisms, all of which will help to accurately deliver the right drug to the right target in the right patient while minimizing side effects. Research Network of Computational and Structural Biotechnology 2019-10-26 /pmc/articles/PMC6945272/ /pubmed/31921397 http://dx.doi.org/10.1016/j.csbj.2019.09.006 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review Article
Colic, Medina
Hart, Traver
Chemogenetic interactions in human cancer cells
title Chemogenetic interactions in human cancer cells
title_full Chemogenetic interactions in human cancer cells
title_fullStr Chemogenetic interactions in human cancer cells
title_full_unstemmed Chemogenetic interactions in human cancer cells
title_short Chemogenetic interactions in human cancer cells
title_sort chemogenetic interactions in human cancer cells
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945272/
https://www.ncbi.nlm.nih.gov/pubmed/31921397
http://dx.doi.org/10.1016/j.csbj.2019.09.006
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