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Chemogenetic interactions in human cancer cells
Chemogenetic profiling enables the identification of genes that enhance or suppress the phenotypic effect of chemical compounds. Using this approach in cancer therapies could improve our ability to predict the response of specific tumor genotypes to chemotherapeutic agents, thus accelerating the dev...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research Network of Computational and Structural Biotechnology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945272/ https://www.ncbi.nlm.nih.gov/pubmed/31921397 http://dx.doi.org/10.1016/j.csbj.2019.09.006 |
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author | Colic, Medina Hart, Traver |
author_facet | Colic, Medina Hart, Traver |
author_sort | Colic, Medina |
collection | PubMed |
description | Chemogenetic profiling enables the identification of genes that enhance or suppress the phenotypic effect of chemical compounds. Using this approach in cancer therapies could improve our ability to predict the response of specific tumor genotypes to chemotherapeutic agents, thus accelerating the development of personalized drug therapy. In the not so distant past, this strategy was only applied in model organisms because there was no feasible technology to thoroughly exploit desired genetic mutations and their impact on drug efficacy in human cells. Today, with the advent of CRISPR gene-editing technology and its application to pooled library screens in mammalian cells, chemogenetic screens are performed directly in human cell lines with high sensitivity and specificity. Chemogenetic profiling provides insights into drug mechanism-of-action, genetic vulnerabilities, and resistance mechanisms, all of which will help to accurately deliver the right drug to the right target in the right patient while minimizing side effects. |
format | Online Article Text |
id | pubmed-6945272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Research Network of Computational and Structural Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-69452722020-01-09 Chemogenetic interactions in human cancer cells Colic, Medina Hart, Traver Comput Struct Biotechnol J Review Article Chemogenetic profiling enables the identification of genes that enhance or suppress the phenotypic effect of chemical compounds. Using this approach in cancer therapies could improve our ability to predict the response of specific tumor genotypes to chemotherapeutic agents, thus accelerating the development of personalized drug therapy. In the not so distant past, this strategy was only applied in model organisms because there was no feasible technology to thoroughly exploit desired genetic mutations and their impact on drug efficacy in human cells. Today, with the advent of CRISPR gene-editing technology and its application to pooled library screens in mammalian cells, chemogenetic screens are performed directly in human cell lines with high sensitivity and specificity. Chemogenetic profiling provides insights into drug mechanism-of-action, genetic vulnerabilities, and resistance mechanisms, all of which will help to accurately deliver the right drug to the right target in the right patient while minimizing side effects. Research Network of Computational and Structural Biotechnology 2019-10-26 /pmc/articles/PMC6945272/ /pubmed/31921397 http://dx.doi.org/10.1016/j.csbj.2019.09.006 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Article Colic, Medina Hart, Traver Chemogenetic interactions in human cancer cells |
title | Chemogenetic interactions in human cancer cells |
title_full | Chemogenetic interactions in human cancer cells |
title_fullStr | Chemogenetic interactions in human cancer cells |
title_full_unstemmed | Chemogenetic interactions in human cancer cells |
title_short | Chemogenetic interactions in human cancer cells |
title_sort | chemogenetic interactions in human cancer cells |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945272/ https://www.ncbi.nlm.nih.gov/pubmed/31921397 http://dx.doi.org/10.1016/j.csbj.2019.09.006 |
work_keys_str_mv | AT colicmedina chemogeneticinteractionsinhumancancercells AT harttraver chemogeneticinteractionsinhumancancercells |