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First report and genetic diversity of porcine bufavirus in China
BACKGROUND: Bufavirus is a newly discovered zoonotic virus reported in numerous mammals and humans. However, the epidemiological and genetic characteristics of porcine bufaviruses (PBuVs) in China remain unclear. METHODS: To detect PBuVs in China, 384 samples (92 fecal and 292 serum specimens) were...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945576/ https://www.ncbi.nlm.nih.gov/pubmed/31906997 http://dx.doi.org/10.1186/s12985-019-1278-6 |
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author | Sun, Yan-Kuo Chen, Yong-Jie Cai, Yu Zhu, Di-Hua Pan, Hao-Ming Wei, Ying-Fang Han, Xiao-Liang Ji, Chi-Hai Lu, Gang Wang, Heng Ma, Chun-Quan Zhang, Gui-Hong |
author_facet | Sun, Yan-Kuo Chen, Yong-Jie Cai, Yu Zhu, Di-Hua Pan, Hao-Ming Wei, Ying-Fang Han, Xiao-Liang Ji, Chi-Hai Lu, Gang Wang, Heng Ma, Chun-Quan Zhang, Gui-Hong |
author_sort | Sun, Yan-Kuo |
collection | PubMed |
description | BACKGROUND: Bufavirus is a newly discovered zoonotic virus reported in numerous mammals and humans. However, the epidemiological and genetic characteristics of porcine bufaviruses (PBuVs) in China remain unclear. METHODS: To detect PBuVs in China, 384 samples (92 fecal and 292 serum specimens) were collected from 2017 to 2018, covering six provinces in China, and were evaluated by nested PCR. Further, the positive samples from different provinces were selected to obtain the complete genome of Chinese PBuVs. RESULTS: The prevalence rate of PBuV was 16.7% in Chinese domestic pigs in the Guangdong, Guangxi, Fujian, Jiangxi, Anhui, and Henan provinces. Additionally, the positive rate of fecal specimens was higher than that of the serum samples. Next, we sequenced nine near-complete genomes of Chinese field PBuV strains from different provinces. Homology and phylogenetic analyses indicated that Chinese PBuVs have high genetic variation (93.3–99.2%), showed higher nucleotide identity with an Austrian PBuV strain (KU867071.1), and developed into different branches within the same cluster. CONCLUSION: To our knowledge, this is the first report on PBuV in China, expanding the geographic boundaries of PBuV circulation. Our data demonstrate that PBuVs are widely distributed in the six Chinese provinces. Moreover, these Chinese PBuVs exhibit genetic variation and continuous evolution characteristics. Taken together, our findings provide a foundation for future studies on bufaviruses. |
format | Online Article Text |
id | pubmed-6945576 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-69455762020-01-07 First report and genetic diversity of porcine bufavirus in China Sun, Yan-Kuo Chen, Yong-Jie Cai, Yu Zhu, Di-Hua Pan, Hao-Ming Wei, Ying-Fang Han, Xiao-Liang Ji, Chi-Hai Lu, Gang Wang, Heng Ma, Chun-Quan Zhang, Gui-Hong Virol J Research BACKGROUND: Bufavirus is a newly discovered zoonotic virus reported in numerous mammals and humans. However, the epidemiological and genetic characteristics of porcine bufaviruses (PBuVs) in China remain unclear. METHODS: To detect PBuVs in China, 384 samples (92 fecal and 292 serum specimens) were collected from 2017 to 2018, covering six provinces in China, and were evaluated by nested PCR. Further, the positive samples from different provinces were selected to obtain the complete genome of Chinese PBuVs. RESULTS: The prevalence rate of PBuV was 16.7% in Chinese domestic pigs in the Guangdong, Guangxi, Fujian, Jiangxi, Anhui, and Henan provinces. Additionally, the positive rate of fecal specimens was higher than that of the serum samples. Next, we sequenced nine near-complete genomes of Chinese field PBuV strains from different provinces. Homology and phylogenetic analyses indicated that Chinese PBuVs have high genetic variation (93.3–99.2%), showed higher nucleotide identity with an Austrian PBuV strain (KU867071.1), and developed into different branches within the same cluster. CONCLUSION: To our knowledge, this is the first report on PBuV in China, expanding the geographic boundaries of PBuV circulation. Our data demonstrate that PBuVs are widely distributed in the six Chinese provinces. Moreover, these Chinese PBuVs exhibit genetic variation and continuous evolution characteristics. Taken together, our findings provide a foundation for future studies on bufaviruses. BioMed Central 2020-01-06 /pmc/articles/PMC6945576/ /pubmed/31906997 http://dx.doi.org/10.1186/s12985-019-1278-6 Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Sun, Yan-Kuo Chen, Yong-Jie Cai, Yu Zhu, Di-Hua Pan, Hao-Ming Wei, Ying-Fang Han, Xiao-Liang Ji, Chi-Hai Lu, Gang Wang, Heng Ma, Chun-Quan Zhang, Gui-Hong First report and genetic diversity of porcine bufavirus in China |
title | First report and genetic diversity of porcine bufavirus in China |
title_full | First report and genetic diversity of porcine bufavirus in China |
title_fullStr | First report and genetic diversity of porcine bufavirus in China |
title_full_unstemmed | First report and genetic diversity of porcine bufavirus in China |
title_short | First report and genetic diversity of porcine bufavirus in China |
title_sort | first report and genetic diversity of porcine bufavirus in china |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945576/ https://www.ncbi.nlm.nih.gov/pubmed/31906997 http://dx.doi.org/10.1186/s12985-019-1278-6 |
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