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Topology and expressed repertoire of the Felis catus T cell receptor loci
BACKGROUND: The domestic cat (Felis catus) is an important companion animal and is used as a large animal model for human disease. However, the comprehensive study of adaptive immunity in this species is hampered by the lack of data on lymphocyte antigen receptor genes and usage. The objectives of t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945721/ https://www.ncbi.nlm.nih.gov/pubmed/31906850 http://dx.doi.org/10.1186/s12864-019-6431-5 |
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author | Radtanakatikanon, Araya Keller, Stefan M. Darzentas, Nikos Moore, Peter F. Folch, Géraldine Nguefack Ngoune, Viviane Lefranc, Marie-Paule Vernau, William |
author_facet | Radtanakatikanon, Araya Keller, Stefan M. Darzentas, Nikos Moore, Peter F. Folch, Géraldine Nguefack Ngoune, Viviane Lefranc, Marie-Paule Vernau, William |
author_sort | Radtanakatikanon, Araya |
collection | PubMed |
description | BACKGROUND: The domestic cat (Felis catus) is an important companion animal and is used as a large animal model for human disease. However, the comprehensive study of adaptive immunity in this species is hampered by the lack of data on lymphocyte antigen receptor genes and usage. The objectives of this study were to annotate the feline T cell receptor (TR) loci and to characterize the expressed repertoire in lymphoid organs of normal cats using high-throughput sequencing. RESULTS: The Felis catus TRG locus contains 30 genes: 12 TRGV, 12 TRGJ and 6 TRGC, the TRB locus contains 48 genes: 33 TRBV, 2 TRBD, 11 TRBJ, 2 TRBC, the TRD locus contains 19 genes: 11 TRDV, 2 TRDD, 5 TRDJ, 1 TRDC, and the TRA locus contains 127 genes: 62 TRAV, 64 TRAJ, 1 TRAC. Functional feline V genes form monophyletic clades with their orthologs, and clustering of multimember subgroups frequently occurs in V genes located at the 5′ end of TR loci. Recombination signal (RS) sequences of the heptamer and nonamer of functional V and J genes are highly conserved. Analysis of the TRG expressed repertoire showed preferential intra-cassette over inter-cassette rearrangements and dominant usage of the TRGV2–1 and TRGJ1–2 genes. The usage of TRBV genes showed minor bias but TRBJ genes of the second J-C-cluster were more commonly rearranged than TRBJ genes of the first cluster. The TRA/TRD V genes almost exclusively rearranged to J genes within their locus. The TRAV/TRAJ gene usage was relatively balanced while the TRD repertoire was dominated by TRDJ3. CONCLUSIONS: This is the first description of all TR loci in the cat. The genomic organization of feline TR loci was similar to that of previously described jawed vertebrates (gnathostomata) and is compatible with the birth-and-death model of evolution. The large-scale characterization of feline TR genes provides comprehensive baseline data on immune repertoires in healthy cats and will facilitate the development of improved reagents for the diagnosis of lymphoproliferative diseases in cats. In addition, these data might benefit studies using cats as a large animal model for human disease. |
format | Online Article Text |
id | pubmed-6945721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-69457212020-01-09 Topology and expressed repertoire of the Felis catus T cell receptor loci Radtanakatikanon, Araya Keller, Stefan M. Darzentas, Nikos Moore, Peter F. Folch, Géraldine Nguefack Ngoune, Viviane Lefranc, Marie-Paule Vernau, William BMC Genomics Research Article BACKGROUND: The domestic cat (Felis catus) is an important companion animal and is used as a large animal model for human disease. However, the comprehensive study of adaptive immunity in this species is hampered by the lack of data on lymphocyte antigen receptor genes and usage. The objectives of this study were to annotate the feline T cell receptor (TR) loci and to characterize the expressed repertoire in lymphoid organs of normal cats using high-throughput sequencing. RESULTS: The Felis catus TRG locus contains 30 genes: 12 TRGV, 12 TRGJ and 6 TRGC, the TRB locus contains 48 genes: 33 TRBV, 2 TRBD, 11 TRBJ, 2 TRBC, the TRD locus contains 19 genes: 11 TRDV, 2 TRDD, 5 TRDJ, 1 TRDC, and the TRA locus contains 127 genes: 62 TRAV, 64 TRAJ, 1 TRAC. Functional feline V genes form monophyletic clades with their orthologs, and clustering of multimember subgroups frequently occurs in V genes located at the 5′ end of TR loci. Recombination signal (RS) sequences of the heptamer and nonamer of functional V and J genes are highly conserved. Analysis of the TRG expressed repertoire showed preferential intra-cassette over inter-cassette rearrangements and dominant usage of the TRGV2–1 and TRGJ1–2 genes. The usage of TRBV genes showed minor bias but TRBJ genes of the second J-C-cluster were more commonly rearranged than TRBJ genes of the first cluster. The TRA/TRD V genes almost exclusively rearranged to J genes within their locus. The TRAV/TRAJ gene usage was relatively balanced while the TRD repertoire was dominated by TRDJ3. CONCLUSIONS: This is the first description of all TR loci in the cat. The genomic organization of feline TR loci was similar to that of previously described jawed vertebrates (gnathostomata) and is compatible with the birth-and-death model of evolution. The large-scale characterization of feline TR genes provides comprehensive baseline data on immune repertoires in healthy cats and will facilitate the development of improved reagents for the diagnosis of lymphoproliferative diseases in cats. In addition, these data might benefit studies using cats as a large animal model for human disease. BioMed Central 2020-01-06 /pmc/articles/PMC6945721/ /pubmed/31906850 http://dx.doi.org/10.1186/s12864-019-6431-5 Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Radtanakatikanon, Araya Keller, Stefan M. Darzentas, Nikos Moore, Peter F. Folch, Géraldine Nguefack Ngoune, Viviane Lefranc, Marie-Paule Vernau, William Topology and expressed repertoire of the Felis catus T cell receptor loci |
title | Topology and expressed repertoire of the Felis catus T cell receptor loci |
title_full | Topology and expressed repertoire of the Felis catus T cell receptor loci |
title_fullStr | Topology and expressed repertoire of the Felis catus T cell receptor loci |
title_full_unstemmed | Topology and expressed repertoire of the Felis catus T cell receptor loci |
title_short | Topology and expressed repertoire of the Felis catus T cell receptor loci |
title_sort | topology and expressed repertoire of the felis catus t cell receptor loci |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945721/ https://www.ncbi.nlm.nih.gov/pubmed/31906850 http://dx.doi.org/10.1186/s12864-019-6431-5 |
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