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Buffer Additives and Packaging Differentially Influence the Stability of Dyes Commonly Used in Chromovitrectomy
PURPOSE: This study was performed to investigate the chemical stability of different dyes used in chromovitrectomy and the influence of various product parameters on it. METHODS: Buffered dye solutions were prepared containing 1.5 g/L acid violet 17, 0.25 g/L brilliant blue G, 1.3 g/L bromophenol bl...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
S. Karger AG
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945961/ https://www.ncbi.nlm.nih.gov/pubmed/31988892 http://dx.doi.org/10.1159/000452241 |
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author | Parrado Aliod, Begoña Kugler, Wilfried Häring, Tim |
author_facet | Parrado Aliod, Begoña Kugler, Wilfried Häring, Tim |
author_sort | Parrado Aliod, Begoña |
collection | PubMed |
description | PURPOSE: This study was performed to investigate the chemical stability of different dyes used in chromovitrectomy and the influence of various product parameters on it. METHODS: Buffered dye solutions were prepared containing 1.5 g/L acid violet 17, 0.25 g/L brilliant blue G, 1.3 g/L bromophenol blue, and 1.5 g/L trypan blue, combined with deuterium oxide, polyethylene glycol 3350, and D-mannitol as additives. For accelerated storage testing, samples were incubated for 400 h at 80°C corresponding to 2 years according to the Van ‘t Hoff equation. After different incubation times samples were taken for UV/Vis spectroscopy, pH measurement, and osmometry. RESULTS: Depending on dye, additive, and packaging, different solutions exhibit differences in chemical stability and hence shelf life. Packaging in syringes instead of vials increases dye stability. Additives may negatively influence important parameters, e.g. polyethylene glycol 3350 increases osmolality beyond the physiological range. Notably, acid violet 17 is chemically unstable except in D-mannitol-containing buffer, packed in syringes. However, simultaneously, D-mannitol leads to a pH shift below 7.0. CONCLUSION: In summary, dye solutions filled in syringes should be preferred to vials to slow down oxidative degradation. Especially acid violet 17 solutions should be used with caution because the addition of D-mannitol may contribute to pH values beyond the physiological range. |
format | Online Article Text |
id | pubmed-6945961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | S. Karger AG |
record_format | MEDLINE/PubMed |
spelling | pubmed-69459612020-01-27 Buffer Additives and Packaging Differentially Influence the Stability of Dyes Commonly Used in Chromovitrectomy Parrado Aliod, Begoña Kugler, Wilfried Häring, Tim Biomed Hub Research Article PURPOSE: This study was performed to investigate the chemical stability of different dyes used in chromovitrectomy and the influence of various product parameters on it. METHODS: Buffered dye solutions were prepared containing 1.5 g/L acid violet 17, 0.25 g/L brilliant blue G, 1.3 g/L bromophenol blue, and 1.5 g/L trypan blue, combined with deuterium oxide, polyethylene glycol 3350, and D-mannitol as additives. For accelerated storage testing, samples were incubated for 400 h at 80°C corresponding to 2 years according to the Van ‘t Hoff equation. After different incubation times samples were taken for UV/Vis spectroscopy, pH measurement, and osmometry. RESULTS: Depending on dye, additive, and packaging, different solutions exhibit differences in chemical stability and hence shelf life. Packaging in syringes instead of vials increases dye stability. Additives may negatively influence important parameters, e.g. polyethylene glycol 3350 increases osmolality beyond the physiological range. Notably, acid violet 17 is chemically unstable except in D-mannitol-containing buffer, packed in syringes. However, simultaneously, D-mannitol leads to a pH shift below 7.0. CONCLUSION: In summary, dye solutions filled in syringes should be preferred to vials to slow down oxidative degradation. Especially acid violet 17 solutions should be used with caution because the addition of D-mannitol may contribute to pH values beyond the physiological range. S. Karger AG 2016-11-16 /pmc/articles/PMC6945961/ /pubmed/31988892 http://dx.doi.org/10.1159/000452241 Text en Copyright © 2016 by S. Karger AG, Basel http://creativecommons.org/licenses/by-nc/4.0/ This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND) (http://www.karger.com/Services/OpenAccessLicense). Usage and distribution for commercial purposes requires written permission. |
spellingShingle | Research Article Parrado Aliod, Begoña Kugler, Wilfried Häring, Tim Buffer Additives and Packaging Differentially Influence the Stability of Dyes Commonly Used in Chromovitrectomy |
title | Buffer Additives and Packaging Differentially Influence the Stability of Dyes Commonly Used in Chromovitrectomy |
title_full | Buffer Additives and Packaging Differentially Influence the Stability of Dyes Commonly Used in Chromovitrectomy |
title_fullStr | Buffer Additives and Packaging Differentially Influence the Stability of Dyes Commonly Used in Chromovitrectomy |
title_full_unstemmed | Buffer Additives and Packaging Differentially Influence the Stability of Dyes Commonly Used in Chromovitrectomy |
title_short | Buffer Additives and Packaging Differentially Influence the Stability of Dyes Commonly Used in Chromovitrectomy |
title_sort | buffer additives and packaging differentially influence the stability of dyes commonly used in chromovitrectomy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945961/ https://www.ncbi.nlm.nih.gov/pubmed/31988892 http://dx.doi.org/10.1159/000452241 |
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