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Pyramiding QTLs controlling tolerance against drought, salinity, and submergence in rice through marker assisted breeding
Increases in rice productivity are significantly hampered because of the increase in the occurrence of abiotic stresses, including drought, salinity, and submergence. Developing a rice variety with inherent tolerance against these major abiotic stresses will help achieve a sustained increase in rice...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946594/ https://www.ncbi.nlm.nih.gov/pubmed/31910435 http://dx.doi.org/10.1371/journal.pone.0227421 |
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author | Muthu, Valarmathi Abbai, Ragavendran Nallathambi, Jagadeeshselvam Rahman, Hifzur Ramasamy, Sasikala Kambale, Rohit Thulasinathan, Thiyagarajan Ayyenar, Bharathi Muthurajan, Raveendran |
author_facet | Muthu, Valarmathi Abbai, Ragavendran Nallathambi, Jagadeeshselvam Rahman, Hifzur Ramasamy, Sasikala Kambale, Rohit Thulasinathan, Thiyagarajan Ayyenar, Bharathi Muthurajan, Raveendran |
author_sort | Muthu, Valarmathi |
collection | PubMed |
description | Increases in rice productivity are significantly hampered because of the increase in the occurrence of abiotic stresses, including drought, salinity, and submergence. Developing a rice variety with inherent tolerance against these major abiotic stresses will help achieve a sustained increase in rice production under unfavorable conditions. The present study was conducted to develop abiotic stress-tolerant rice genotypes in the genetic background of the popular rice variety Improved White Ponni (IWP) by introgressing major effect quantitative trait loci (QTLs) conferring tolerance against drought (qDTY(1.1), qDTY(2.1)), salinity (Saltol), and submergence (Sub1) through a marker assisted backcross breeding approach. Genotyping of early generation backcrossed inbred lines (BILs) resulted in the identification of three progenies, 3-11-9-2, 3-11-11-1, and 3-11-11-2, possessing all four target QTLs and maximum recovery of the recurrent parent genome (88.46%). BILs exhibited consistent agronomic and grain quality characters compared to those of IWP and enhanced performance against dehydration, salinity, and submergence stress compared with the recurrent parent IWP. BILs exhibited enhanced tolerance against salinity during germination and increased shoot length, root length, and vigor index compared to those of IWP. All three BILs exhibited reduced symptoms of injury because of salinity (NaCl) and dehydration (PEG) than did IWP. At 12 days of submergence stress, BILs exhibited enhanced survival and greater recovery, whereas IWP failed completely. BILs were found to exhibit on par grain and cooking quality characteristics with their parents. Results of this study clearly demonstrated the effects of the target QTLs in reducing damage caused by drought, salinity, and submergence and lead to the development of a triple stress tolerant version of IWP. |
format | Online Article Text |
id | pubmed-6946594 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-69465942020-01-17 Pyramiding QTLs controlling tolerance against drought, salinity, and submergence in rice through marker assisted breeding Muthu, Valarmathi Abbai, Ragavendran Nallathambi, Jagadeeshselvam Rahman, Hifzur Ramasamy, Sasikala Kambale, Rohit Thulasinathan, Thiyagarajan Ayyenar, Bharathi Muthurajan, Raveendran PLoS One Research Article Increases in rice productivity are significantly hampered because of the increase in the occurrence of abiotic stresses, including drought, salinity, and submergence. Developing a rice variety with inherent tolerance against these major abiotic stresses will help achieve a sustained increase in rice production under unfavorable conditions. The present study was conducted to develop abiotic stress-tolerant rice genotypes in the genetic background of the popular rice variety Improved White Ponni (IWP) by introgressing major effect quantitative trait loci (QTLs) conferring tolerance against drought (qDTY(1.1), qDTY(2.1)), salinity (Saltol), and submergence (Sub1) through a marker assisted backcross breeding approach. Genotyping of early generation backcrossed inbred lines (BILs) resulted in the identification of three progenies, 3-11-9-2, 3-11-11-1, and 3-11-11-2, possessing all four target QTLs and maximum recovery of the recurrent parent genome (88.46%). BILs exhibited consistent agronomic and grain quality characters compared to those of IWP and enhanced performance against dehydration, salinity, and submergence stress compared with the recurrent parent IWP. BILs exhibited enhanced tolerance against salinity during germination and increased shoot length, root length, and vigor index compared to those of IWP. All three BILs exhibited reduced symptoms of injury because of salinity (NaCl) and dehydration (PEG) than did IWP. At 12 days of submergence stress, BILs exhibited enhanced survival and greater recovery, whereas IWP failed completely. BILs were found to exhibit on par grain and cooking quality characteristics with their parents. Results of this study clearly demonstrated the effects of the target QTLs in reducing damage caused by drought, salinity, and submergence and lead to the development of a triple stress tolerant version of IWP. Public Library of Science 2020-01-07 /pmc/articles/PMC6946594/ /pubmed/31910435 http://dx.doi.org/10.1371/journal.pone.0227421 Text en © 2020 Muthu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Muthu, Valarmathi Abbai, Ragavendran Nallathambi, Jagadeeshselvam Rahman, Hifzur Ramasamy, Sasikala Kambale, Rohit Thulasinathan, Thiyagarajan Ayyenar, Bharathi Muthurajan, Raveendran Pyramiding QTLs controlling tolerance against drought, salinity, and submergence in rice through marker assisted breeding |
title | Pyramiding QTLs controlling tolerance against drought, salinity, and submergence in rice through marker assisted breeding |
title_full | Pyramiding QTLs controlling tolerance against drought, salinity, and submergence in rice through marker assisted breeding |
title_fullStr | Pyramiding QTLs controlling tolerance against drought, salinity, and submergence in rice through marker assisted breeding |
title_full_unstemmed | Pyramiding QTLs controlling tolerance against drought, salinity, and submergence in rice through marker assisted breeding |
title_short | Pyramiding QTLs controlling tolerance against drought, salinity, and submergence in rice through marker assisted breeding |
title_sort | pyramiding qtls controlling tolerance against drought, salinity, and submergence in rice through marker assisted breeding |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946594/ https://www.ncbi.nlm.nih.gov/pubmed/31910435 http://dx.doi.org/10.1371/journal.pone.0227421 |
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