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microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer

Background: Gastric cancer (GC) is the one of most common malignancies and its mechanism of metastasis remains unclear. The study was designed to investigate the effects of microRNA-217 on epithelial-to-mesenchymal transition. Methods: The expression levels of miR-217 in GC were assayed by real-time...

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Detalles Bibliográficos
Autores principales: Chen, Gen, Yang, Zhangshuo, Feng, Maohui, Wang, Zhiliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946620/
https://www.ncbi.nlm.nih.gov/pubmed/31793993
http://dx.doi.org/10.1042/BSR20193176
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author Chen, Gen
Yang, Zhangshuo
Feng, Maohui
Wang, Zhiliang
author_facet Chen, Gen
Yang, Zhangshuo
Feng, Maohui
Wang, Zhiliang
author_sort Chen, Gen
collection PubMed
description Background: Gastric cancer (GC) is the one of most common malignancies and its mechanism of metastasis remains unclear. The study was designed to investigate the effects of microRNA-217 on epithelial-to-mesenchymal transition. Methods: The expression levels of miR-217 in GC were assayed by real-time qPCR. Metastasis and invasion of cancer cell were assayed by transwell chamber. Double luciferase reporter gene was used to verify the target regulatory relationship between microRNA-217 and tyrosine–protein phosphatase non-receptor type 14 (PTPN14) on gastric cell lines. Epithelial-to-mesenchymal transition (EMT) markers were assayed by Western blot. Results: We found that miR-217 had a low level expression in gastric tumor tissues of 40 patients with GC, and a lower expression in the gastric tumor tissues of the patients with GC metastasis. Moreover, miR-217 markedly suppressed the metastasis and invasion of gastric cancer cell line in vitro. Furthermore, miR-217 inhibited the expression of PTPN14 by directly targeting its 3′UTR. Moreover, the down-regulation of PTPN14 reduced the metastasis and invasion, whereas up-regulation of PTPN14 led to the enhanced metastases and invasion of gastric cells. miR-217 induced the down-regulation of PTPN14 and inhibited the EMT in gastric cancer cells. Conclusion: miR-217 inhibited the EMT through directly targeting to the 3′UTR of PTPN14.
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spelling pubmed-69466202020-01-15 microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer Chen, Gen Yang, Zhangshuo Feng, Maohui Wang, Zhiliang Biosci Rep Cancer Background: Gastric cancer (GC) is the one of most common malignancies and its mechanism of metastasis remains unclear. The study was designed to investigate the effects of microRNA-217 on epithelial-to-mesenchymal transition. Methods: The expression levels of miR-217 in GC were assayed by real-time qPCR. Metastasis and invasion of cancer cell were assayed by transwell chamber. Double luciferase reporter gene was used to verify the target regulatory relationship between microRNA-217 and tyrosine–protein phosphatase non-receptor type 14 (PTPN14) on gastric cell lines. Epithelial-to-mesenchymal transition (EMT) markers were assayed by Western blot. Results: We found that miR-217 had a low level expression in gastric tumor tissues of 40 patients with GC, and a lower expression in the gastric tumor tissues of the patients with GC metastasis. Moreover, miR-217 markedly suppressed the metastasis and invasion of gastric cancer cell line in vitro. Furthermore, miR-217 inhibited the expression of PTPN14 by directly targeting its 3′UTR. Moreover, the down-regulation of PTPN14 reduced the metastasis and invasion, whereas up-regulation of PTPN14 led to the enhanced metastases and invasion of gastric cells. miR-217 induced the down-regulation of PTPN14 and inhibited the EMT in gastric cancer cells. Conclusion: miR-217 inhibited the EMT through directly targeting to the 3′UTR of PTPN14. Portland Press Ltd. 2020-01-07 /pmc/articles/PMC6946620/ /pubmed/31793993 http://dx.doi.org/10.1042/BSR20193176 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
spellingShingle Cancer
Chen, Gen
Yang, Zhangshuo
Feng, Maohui
Wang, Zhiliang
microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer
title microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer
title_full microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer
title_fullStr microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer
title_full_unstemmed microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer
title_short microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer
title_sort microrna-217 suppressed epithelial-to-mesenchymal transition through targeting ptpn14 in gastric cancer
topic Cancer
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946620/
https://www.ncbi.nlm.nih.gov/pubmed/31793993
http://dx.doi.org/10.1042/BSR20193176
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