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microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer
Background: Gastric cancer (GC) is the one of most common malignancies and its mechanism of metastasis remains unclear. The study was designed to investigate the effects of microRNA-217 on epithelial-to-mesenchymal transition. Methods: The expression levels of miR-217 in GC were assayed by real-time...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946620/ https://www.ncbi.nlm.nih.gov/pubmed/31793993 http://dx.doi.org/10.1042/BSR20193176 |
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author | Chen, Gen Yang, Zhangshuo Feng, Maohui Wang, Zhiliang |
author_facet | Chen, Gen Yang, Zhangshuo Feng, Maohui Wang, Zhiliang |
author_sort | Chen, Gen |
collection | PubMed |
description | Background: Gastric cancer (GC) is the one of most common malignancies and its mechanism of metastasis remains unclear. The study was designed to investigate the effects of microRNA-217 on epithelial-to-mesenchymal transition. Methods: The expression levels of miR-217 in GC were assayed by real-time qPCR. Metastasis and invasion of cancer cell were assayed by transwell chamber. Double luciferase reporter gene was used to verify the target regulatory relationship between microRNA-217 and tyrosine–protein phosphatase non-receptor type 14 (PTPN14) on gastric cell lines. Epithelial-to-mesenchymal transition (EMT) markers were assayed by Western blot. Results: We found that miR-217 had a low level expression in gastric tumor tissues of 40 patients with GC, and a lower expression in the gastric tumor tissues of the patients with GC metastasis. Moreover, miR-217 markedly suppressed the metastasis and invasion of gastric cancer cell line in vitro. Furthermore, miR-217 inhibited the expression of PTPN14 by directly targeting its 3′UTR. Moreover, the down-regulation of PTPN14 reduced the metastasis and invasion, whereas up-regulation of PTPN14 led to the enhanced metastases and invasion of gastric cells. miR-217 induced the down-regulation of PTPN14 and inhibited the EMT in gastric cancer cells. Conclusion: miR-217 inhibited the EMT through directly targeting to the 3′UTR of PTPN14. |
format | Online Article Text |
id | pubmed-6946620 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69466202020-01-15 microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer Chen, Gen Yang, Zhangshuo Feng, Maohui Wang, Zhiliang Biosci Rep Cancer Background: Gastric cancer (GC) is the one of most common malignancies and its mechanism of metastasis remains unclear. The study was designed to investigate the effects of microRNA-217 on epithelial-to-mesenchymal transition. Methods: The expression levels of miR-217 in GC were assayed by real-time qPCR. Metastasis and invasion of cancer cell were assayed by transwell chamber. Double luciferase reporter gene was used to verify the target regulatory relationship between microRNA-217 and tyrosine–protein phosphatase non-receptor type 14 (PTPN14) on gastric cell lines. Epithelial-to-mesenchymal transition (EMT) markers were assayed by Western blot. Results: We found that miR-217 had a low level expression in gastric tumor tissues of 40 patients with GC, and a lower expression in the gastric tumor tissues of the patients with GC metastasis. Moreover, miR-217 markedly suppressed the metastasis and invasion of gastric cancer cell line in vitro. Furthermore, miR-217 inhibited the expression of PTPN14 by directly targeting its 3′UTR. Moreover, the down-regulation of PTPN14 reduced the metastasis and invasion, whereas up-regulation of PTPN14 led to the enhanced metastases and invasion of gastric cells. miR-217 induced the down-regulation of PTPN14 and inhibited the EMT in gastric cancer cells. Conclusion: miR-217 inhibited the EMT through directly targeting to the 3′UTR of PTPN14. Portland Press Ltd. 2020-01-07 /pmc/articles/PMC6946620/ /pubmed/31793993 http://dx.doi.org/10.1042/BSR20193176 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
spellingShingle | Cancer Chen, Gen Yang, Zhangshuo Feng, Maohui Wang, Zhiliang microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer |
title | microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer |
title_full | microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer |
title_fullStr | microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer |
title_full_unstemmed | microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer |
title_short | microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer |
title_sort | microrna-217 suppressed epithelial-to-mesenchymal transition through targeting ptpn14 in gastric cancer |
topic | Cancer |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946620/ https://www.ncbi.nlm.nih.gov/pubmed/31793993 http://dx.doi.org/10.1042/BSR20193176 |
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