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Effects of centrifugation and whole-body vibrations on blood–brain barrier permeability in mice

Modifications of gravity levels induce generalized adaptation of mammalian physiology, including vascular, brain, muscle, bone and immunity functions. As a crucial interface between the vascular system and the brain, the blood–brain barrier (BBB) acts as a filter to protect neurons from pathogens an...

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Autores principales: Dubayle, David, Vanden-Bossche, Arnaud, Beraneck, Mathieu, Vico, Laurence, Morel, Jean-Luc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946672/
https://www.ncbi.nlm.nih.gov/pubmed/31934612
http://dx.doi.org/10.1038/s41526-019-0094-z
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author Dubayle, David
Vanden-Bossche, Arnaud
Beraneck, Mathieu
Vico, Laurence
Morel, Jean-Luc
author_facet Dubayle, David
Vanden-Bossche, Arnaud
Beraneck, Mathieu
Vico, Laurence
Morel, Jean-Luc
author_sort Dubayle, David
collection PubMed
description Modifications of gravity levels induce generalized adaptation of mammalian physiology, including vascular, brain, muscle, bone and immunity functions. As a crucial interface between the vascular system and the brain, the blood–brain barrier (BBB) acts as a filter to protect neurons from pathogens and inflammation. Here we compare the effects of several protocols of hypergravity induced by centrifugation and whole-body vibrations (WBV) on BBB integrity. The immunohistochemistry revealed immunoglobulin G (IgG) extravasation from blood to hippocampal parenchyma of mice centrifuged at 2 × g during 1 or 50 days, whereas short exposures to higher hypergravity mimicking the profiles of spaceflight landing and take-off (short exposures to 5 × g) had no effects. These results suggest prolonged centrifugation (>1 days) at 2 × g induced a BBB leakage. Moreover, WBV were similarly tested. The short exposure to +2 × g vibrations (900 s/day at 90 Hz) repeated for 63 days induced IgG extravasation in hippocampal parenchyma, whereas the progressive increase of vibrations from +0.5 to +2 × g for 63 days was not able to affect the IgG crossing through the BBB. Overall, these results suggest that the BBB permeability is sensitive to prolonged external accelerations. In conclusion, we advise that the protocols of WBV and centrifugation, proposed as countermeasure to spaceflight, should be designed with progressively increasing exposure to reduce potential side effects on the BBB.
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spelling pubmed-69466722020-01-13 Effects of centrifugation and whole-body vibrations on blood–brain barrier permeability in mice Dubayle, David Vanden-Bossche, Arnaud Beraneck, Mathieu Vico, Laurence Morel, Jean-Luc NPJ Microgravity Article Modifications of gravity levels induce generalized adaptation of mammalian physiology, including vascular, brain, muscle, bone and immunity functions. As a crucial interface between the vascular system and the brain, the blood–brain barrier (BBB) acts as a filter to protect neurons from pathogens and inflammation. Here we compare the effects of several protocols of hypergravity induced by centrifugation and whole-body vibrations (WBV) on BBB integrity. The immunohistochemistry revealed immunoglobulin G (IgG) extravasation from blood to hippocampal parenchyma of mice centrifuged at 2 × g during 1 or 50 days, whereas short exposures to higher hypergravity mimicking the profiles of spaceflight landing and take-off (short exposures to 5 × g) had no effects. These results suggest prolonged centrifugation (>1 days) at 2 × g induced a BBB leakage. Moreover, WBV were similarly tested. The short exposure to +2 × g vibrations (900 s/day at 90 Hz) repeated for 63 days induced IgG extravasation in hippocampal parenchyma, whereas the progressive increase of vibrations from +0.5 to +2 × g for 63 days was not able to affect the IgG crossing through the BBB. Overall, these results suggest that the BBB permeability is sensitive to prolonged external accelerations. In conclusion, we advise that the protocols of WBV and centrifugation, proposed as countermeasure to spaceflight, should be designed with progressively increasing exposure to reduce potential side effects on the BBB. Nature Publishing Group UK 2020-01-07 /pmc/articles/PMC6946672/ /pubmed/31934612 http://dx.doi.org/10.1038/s41526-019-0094-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dubayle, David
Vanden-Bossche, Arnaud
Beraneck, Mathieu
Vico, Laurence
Morel, Jean-Luc
Effects of centrifugation and whole-body vibrations on blood–brain barrier permeability in mice
title Effects of centrifugation and whole-body vibrations on blood–brain barrier permeability in mice
title_full Effects of centrifugation and whole-body vibrations on blood–brain barrier permeability in mice
title_fullStr Effects of centrifugation and whole-body vibrations on blood–brain barrier permeability in mice
title_full_unstemmed Effects of centrifugation and whole-body vibrations on blood–brain barrier permeability in mice
title_short Effects of centrifugation and whole-body vibrations on blood–brain barrier permeability in mice
title_sort effects of centrifugation and whole-body vibrations on blood–brain barrier permeability in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946672/
https://www.ncbi.nlm.nih.gov/pubmed/31934612
http://dx.doi.org/10.1038/s41526-019-0094-z
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