Cargando…

A Novel Arsenate-Resistant Determinant Associated with ICEpMERPH, a Member of the SXT/R391 Group of Mobile Genetic Elements

ICEpMERPH, the first integrative conjugative element (ICE) of the SXT/R391 family isolated in the United Kingdom and Europe, was analyzed to determine the nature of its adaptive functions, its genetic structure, and its homology to related elements normally found in pathogenic Vibrio or Proteus spec...

Descripción completa

Detalles Bibliográficos
Autores principales: Ryan, Michael P., Slattery, Shannon, Pembroke, J. Tony
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947025/
https://www.ncbi.nlm.nih.gov/pubmed/31888308
http://dx.doi.org/10.3390/genes10121048
_version_ 1783485474060369920
author Ryan, Michael P.
Slattery, Shannon
Pembroke, J. Tony
author_facet Ryan, Michael P.
Slattery, Shannon
Pembroke, J. Tony
author_sort Ryan, Michael P.
collection PubMed
description ICEpMERPH, the first integrative conjugative element (ICE) of the SXT/R391 family isolated in the United Kingdom and Europe, was analyzed to determine the nature of its adaptive functions, its genetic structure, and its homology to related elements normally found in pathogenic Vibrio or Proteus species. Whole genome sequencing of Escherichia coli (E. coli) isolate K802 (which contains the ICEpMERPH) was carried out using Illumina sequencing technology. ICEpMERPH has a size of 110 Kb and 112 putative open reading frames (ORFs). The “hotspot regions” of the element were found to contain putative restriction digestion systems, insertion sequences, and heavy metal resistance genes that encoded resistance to mercury, as previously reported, but also surprisingly to arsenate. A novel arsenate resistance system was identified in hotspot 4 of the element, unrelated to other SXT/R391 elements. This arsenate resistance system was potentially linked to two genes: orf69, encoding an organoarsenical efflux major facilitator superfamily (MFS) transporter-like protein related to ArsJ, and orf70, encoding nicotinamide adenine dinucleotide (NAD)-dependent glyceraldehyde-3-phosphate dehydrogenase. Phenotypic analysis using isogenic strains of Escherichia coli strain AB1157 with and without the ICEpMERPH revealed resistance to low levels of arsenate in the range of 1–5 mM. This novel, low-level resistance may have an important adaptive function in polluted environments, which often contain low levels of arsenate contamination. A bioinformatic analysis on the novel determinant and the phylogeny of ICEpMERPH was presented.
format Online
Article
Text
id pubmed-6947025
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69470252020-01-13 A Novel Arsenate-Resistant Determinant Associated with ICEpMERPH, a Member of the SXT/R391 Group of Mobile Genetic Elements Ryan, Michael P. Slattery, Shannon Pembroke, J. Tony Genes (Basel) Article ICEpMERPH, the first integrative conjugative element (ICE) of the SXT/R391 family isolated in the United Kingdom and Europe, was analyzed to determine the nature of its adaptive functions, its genetic structure, and its homology to related elements normally found in pathogenic Vibrio or Proteus species. Whole genome sequencing of Escherichia coli (E. coli) isolate K802 (which contains the ICEpMERPH) was carried out using Illumina sequencing technology. ICEpMERPH has a size of 110 Kb and 112 putative open reading frames (ORFs). The “hotspot regions” of the element were found to contain putative restriction digestion systems, insertion sequences, and heavy metal resistance genes that encoded resistance to mercury, as previously reported, but also surprisingly to arsenate. A novel arsenate resistance system was identified in hotspot 4 of the element, unrelated to other SXT/R391 elements. This arsenate resistance system was potentially linked to two genes: orf69, encoding an organoarsenical efflux major facilitator superfamily (MFS) transporter-like protein related to ArsJ, and orf70, encoding nicotinamide adenine dinucleotide (NAD)-dependent glyceraldehyde-3-phosphate dehydrogenase. Phenotypic analysis using isogenic strains of Escherichia coli strain AB1157 with and without the ICEpMERPH revealed resistance to low levels of arsenate in the range of 1–5 mM. This novel, low-level resistance may have an important adaptive function in polluted environments, which often contain low levels of arsenate contamination. A bioinformatic analysis on the novel determinant and the phylogeny of ICEpMERPH was presented. MDPI 2019-12-16 /pmc/articles/PMC6947025/ /pubmed/31888308 http://dx.doi.org/10.3390/genes10121048 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ryan, Michael P.
Slattery, Shannon
Pembroke, J. Tony
A Novel Arsenate-Resistant Determinant Associated with ICEpMERPH, a Member of the SXT/R391 Group of Mobile Genetic Elements
title A Novel Arsenate-Resistant Determinant Associated with ICEpMERPH, a Member of the SXT/R391 Group of Mobile Genetic Elements
title_full A Novel Arsenate-Resistant Determinant Associated with ICEpMERPH, a Member of the SXT/R391 Group of Mobile Genetic Elements
title_fullStr A Novel Arsenate-Resistant Determinant Associated with ICEpMERPH, a Member of the SXT/R391 Group of Mobile Genetic Elements
title_full_unstemmed A Novel Arsenate-Resistant Determinant Associated with ICEpMERPH, a Member of the SXT/R391 Group of Mobile Genetic Elements
title_short A Novel Arsenate-Resistant Determinant Associated with ICEpMERPH, a Member of the SXT/R391 Group of Mobile Genetic Elements
title_sort novel arsenate-resistant determinant associated with icepmerph, a member of the sxt/r391 group of mobile genetic elements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947025/
https://www.ncbi.nlm.nih.gov/pubmed/31888308
http://dx.doi.org/10.3390/genes10121048
work_keys_str_mv AT ryanmichaelp anovelarsenateresistantdeterminantassociatedwithicepmerphamemberofthesxtr391groupofmobilegeneticelements
AT slatteryshannon anovelarsenateresistantdeterminantassociatedwithicepmerphamemberofthesxtr391groupofmobilegeneticelements
AT pembrokejtony anovelarsenateresistantdeterminantassociatedwithicepmerphamemberofthesxtr391groupofmobilegeneticelements
AT ryanmichaelp novelarsenateresistantdeterminantassociatedwithicepmerphamemberofthesxtr391groupofmobilegeneticelements
AT slatteryshannon novelarsenateresistantdeterminantassociatedwithicepmerphamemberofthesxtr391groupofmobilegeneticelements
AT pembrokejtony novelarsenateresistantdeterminantassociatedwithicepmerphamemberofthesxtr391groupofmobilegeneticelements