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Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice

Ulcerative colitis is a relatively frequent, chronic disease that impacts significantly the patient’s quality of life. Although many therapeutic options are available, additional approaches are needed because many patients either do not respond to current therapies or show significant side effects....

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Autores principales: Sánchez-Garrido, Ana I., Prieto-Vicente, Vanessa, Blanco-Gozalo, Víctor, Arévalo, Miguel, Quiros, Yaremi, López-Montañés, Daniel, López-Hernández, Francisco J., Rodríguez-Pérez, Antonio, López-Novoa, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947259/
https://www.ncbi.nlm.nih.gov/pubmed/31805674
http://dx.doi.org/10.3390/jcm8122086
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author Sánchez-Garrido, Ana I.
Prieto-Vicente, Vanessa
Blanco-Gozalo, Víctor
Arévalo, Miguel
Quiros, Yaremi
López-Montañés, Daniel
López-Hernández, Francisco J.
Rodríguez-Pérez, Antonio
López-Novoa, José M.
author_facet Sánchez-Garrido, Ana I.
Prieto-Vicente, Vanessa
Blanco-Gozalo, Víctor
Arévalo, Miguel
Quiros, Yaremi
López-Montañés, Daniel
López-Hernández, Francisco J.
Rodríguez-Pérez, Antonio
López-Novoa, José M.
author_sort Sánchez-Garrido, Ana I.
collection PubMed
description Ulcerative colitis is a relatively frequent, chronic disease that impacts significantly the patient’s quality of life. Although many therapeutic options are available, additional approaches are needed because many patients either do not respond to current therapies or show significant side effects. Cardiotrophin-1 (CT-1) is a cytokine with potent cytoprotective, anti-inflammatory, and antiapoptotic properties. The purpose of this study was to assess if the administration of CT-1 could reduce colon damage in mice with experimental colitis was induced with 5% dextran sulfate sodium (DSS) in the drinking water. Half of the mice received an i.v. dose of CT-1 (200 µg/kg) 2 h before and 2 and 4 days after DSS administration. Animals were followed during 7 days after DSS administration. The severity of colitis was measured by standard scores. Colon damage was assessed by histology and immunohistochemistry. Inflammatory mediators were measured by Western blot and PCR. CT-1 administration to DSS-treated mice ameliorated both the clinical course (disease activity index), histological damage, inflammation (colon expression of TNF-α, IL-17, IL-10, INF IFN-γ, and iNOS), and apoptosis. Our results suggest that CT-1 administration before induction of colitis improves the clinical course, tissue damage, and inflammation in DSS-induced colitis in mice.
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spelling pubmed-69472592020-01-13 Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice Sánchez-Garrido, Ana I. Prieto-Vicente, Vanessa Blanco-Gozalo, Víctor Arévalo, Miguel Quiros, Yaremi López-Montañés, Daniel López-Hernández, Francisco J. Rodríguez-Pérez, Antonio López-Novoa, José M. J Clin Med Article Ulcerative colitis is a relatively frequent, chronic disease that impacts significantly the patient’s quality of life. Although many therapeutic options are available, additional approaches are needed because many patients either do not respond to current therapies or show significant side effects. Cardiotrophin-1 (CT-1) is a cytokine with potent cytoprotective, anti-inflammatory, and antiapoptotic properties. The purpose of this study was to assess if the administration of CT-1 could reduce colon damage in mice with experimental colitis was induced with 5% dextran sulfate sodium (DSS) in the drinking water. Half of the mice received an i.v. dose of CT-1 (200 µg/kg) 2 h before and 2 and 4 days after DSS administration. Animals were followed during 7 days after DSS administration. The severity of colitis was measured by standard scores. Colon damage was assessed by histology and immunohistochemistry. Inflammatory mediators were measured by Western blot and PCR. CT-1 administration to DSS-treated mice ameliorated both the clinical course (disease activity index), histological damage, inflammation (colon expression of TNF-α, IL-17, IL-10, INF IFN-γ, and iNOS), and apoptosis. Our results suggest that CT-1 administration before induction of colitis improves the clinical course, tissue damage, and inflammation in DSS-induced colitis in mice. MDPI 2019-12-01 /pmc/articles/PMC6947259/ /pubmed/31805674 http://dx.doi.org/10.3390/jcm8122086 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sánchez-Garrido, Ana I.
Prieto-Vicente, Vanessa
Blanco-Gozalo, Víctor
Arévalo, Miguel
Quiros, Yaremi
López-Montañés, Daniel
López-Hernández, Francisco J.
Rodríguez-Pérez, Antonio
López-Novoa, José M.
Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice
title Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice
title_full Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice
title_fullStr Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice
title_full_unstemmed Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice
title_short Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice
title_sort preventive effect of cardiotrophin-1 administration before dss-induced ulcerative colitis in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947259/
https://www.ncbi.nlm.nih.gov/pubmed/31805674
http://dx.doi.org/10.3390/jcm8122086
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