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Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice
Ulcerative colitis is a relatively frequent, chronic disease that impacts significantly the patient’s quality of life. Although many therapeutic options are available, additional approaches are needed because many patients either do not respond to current therapies or show significant side effects....
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947259/ https://www.ncbi.nlm.nih.gov/pubmed/31805674 http://dx.doi.org/10.3390/jcm8122086 |
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author | Sánchez-Garrido, Ana I. Prieto-Vicente, Vanessa Blanco-Gozalo, Víctor Arévalo, Miguel Quiros, Yaremi López-Montañés, Daniel López-Hernández, Francisco J. Rodríguez-Pérez, Antonio López-Novoa, José M. |
author_facet | Sánchez-Garrido, Ana I. Prieto-Vicente, Vanessa Blanco-Gozalo, Víctor Arévalo, Miguel Quiros, Yaremi López-Montañés, Daniel López-Hernández, Francisco J. Rodríguez-Pérez, Antonio López-Novoa, José M. |
author_sort | Sánchez-Garrido, Ana I. |
collection | PubMed |
description | Ulcerative colitis is a relatively frequent, chronic disease that impacts significantly the patient’s quality of life. Although many therapeutic options are available, additional approaches are needed because many patients either do not respond to current therapies or show significant side effects. Cardiotrophin-1 (CT-1) is a cytokine with potent cytoprotective, anti-inflammatory, and antiapoptotic properties. The purpose of this study was to assess if the administration of CT-1 could reduce colon damage in mice with experimental colitis was induced with 5% dextran sulfate sodium (DSS) in the drinking water. Half of the mice received an i.v. dose of CT-1 (200 µg/kg) 2 h before and 2 and 4 days after DSS administration. Animals were followed during 7 days after DSS administration. The severity of colitis was measured by standard scores. Colon damage was assessed by histology and immunohistochemistry. Inflammatory mediators were measured by Western blot and PCR. CT-1 administration to DSS-treated mice ameliorated both the clinical course (disease activity index), histological damage, inflammation (colon expression of TNF-α, IL-17, IL-10, INF IFN-γ, and iNOS), and apoptosis. Our results suggest that CT-1 administration before induction of colitis improves the clinical course, tissue damage, and inflammation in DSS-induced colitis in mice. |
format | Online Article Text |
id | pubmed-6947259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69472592020-01-13 Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice Sánchez-Garrido, Ana I. Prieto-Vicente, Vanessa Blanco-Gozalo, Víctor Arévalo, Miguel Quiros, Yaremi López-Montañés, Daniel López-Hernández, Francisco J. Rodríguez-Pérez, Antonio López-Novoa, José M. J Clin Med Article Ulcerative colitis is a relatively frequent, chronic disease that impacts significantly the patient’s quality of life. Although many therapeutic options are available, additional approaches are needed because many patients either do not respond to current therapies or show significant side effects. Cardiotrophin-1 (CT-1) is a cytokine with potent cytoprotective, anti-inflammatory, and antiapoptotic properties. The purpose of this study was to assess if the administration of CT-1 could reduce colon damage in mice with experimental colitis was induced with 5% dextran sulfate sodium (DSS) in the drinking water. Half of the mice received an i.v. dose of CT-1 (200 µg/kg) 2 h before and 2 and 4 days after DSS administration. Animals were followed during 7 days after DSS administration. The severity of colitis was measured by standard scores. Colon damage was assessed by histology and immunohistochemistry. Inflammatory mediators were measured by Western blot and PCR. CT-1 administration to DSS-treated mice ameliorated both the clinical course (disease activity index), histological damage, inflammation (colon expression of TNF-α, IL-17, IL-10, INF IFN-γ, and iNOS), and apoptosis. Our results suggest that CT-1 administration before induction of colitis improves the clinical course, tissue damage, and inflammation in DSS-induced colitis in mice. MDPI 2019-12-01 /pmc/articles/PMC6947259/ /pubmed/31805674 http://dx.doi.org/10.3390/jcm8122086 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sánchez-Garrido, Ana I. Prieto-Vicente, Vanessa Blanco-Gozalo, Víctor Arévalo, Miguel Quiros, Yaremi López-Montañés, Daniel López-Hernández, Francisco J. Rodríguez-Pérez, Antonio López-Novoa, José M. Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice |
title | Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice |
title_full | Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice |
title_fullStr | Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice |
title_full_unstemmed | Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice |
title_short | Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice |
title_sort | preventive effect of cardiotrophin-1 administration before dss-induced ulcerative colitis in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947259/ https://www.ncbi.nlm.nih.gov/pubmed/31805674 http://dx.doi.org/10.3390/jcm8122086 |
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