Cargando…

Photoactive Pore Matrix for In Situ Delivery of a Photosensitizer in Vascular Smooth Muscle Cells Selective PDT

In this study we present the porous silica-based material that can be used for in situ drug delivery, offering effective supply of active compounds regardless its water solubility. To demonstrate usability of this new material, three silica-based materials with different pore size distribution as a...

Descripción completa

Detalles Bibliográficos
Autores principales: Wawrzyńska, Magdalena, Duda, Maciej, Hołowacz, Iwona, Kaczorowska, Aleksandra, Ulatowska-Jarża, Agnieszka, Buzalewicz, Igor, Kałas, Wojciech, Wysokińska, Edyta, Biały, Dariusz, Podbielska, Halina, Kopaczyńska, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947284/
https://www.ncbi.nlm.nih.gov/pubmed/31818025
http://dx.doi.org/10.3390/ma12244110
_version_ 1783485514775527424
author Wawrzyńska, Magdalena
Duda, Maciej
Hołowacz, Iwona
Kaczorowska, Aleksandra
Ulatowska-Jarża, Agnieszka
Buzalewicz, Igor
Kałas, Wojciech
Wysokińska, Edyta
Biały, Dariusz
Podbielska, Halina
Kopaczyńska, Marta
author_facet Wawrzyńska, Magdalena
Duda, Maciej
Hołowacz, Iwona
Kaczorowska, Aleksandra
Ulatowska-Jarża, Agnieszka
Buzalewicz, Igor
Kałas, Wojciech
Wysokińska, Edyta
Biały, Dariusz
Podbielska, Halina
Kopaczyńska, Marta
author_sort Wawrzyńska, Magdalena
collection PubMed
description In this study we present the porous silica-based material that can be used for in situ drug delivery, offering effective supply of active compounds regardless its water solubility. To demonstrate usability of this new material, three silica-based materials with different pore size distribution as a matrix for doping with Photolon (Ph) and Protoporphyrin IX (PPIX) photosensitizers, were prepared. These matrices can be used for coating cardiovascular stents used for treatment of the coronary artery disease and enable intravascular photodynamic therapy (PDT), which can modulate the vascular response to injury caused by stent implantation—procedure that should be thought as an alternative for drug eluting stent. The FTIR spectroscopic analysis confirmed that all studied matrices have been successfully functionalized with the target photosensitizers. Atomic force microscopy revealed that resulting photoactive matrices were very smooth, which can limit the implantation damage and reduce the risk of restenosis. No viability loss of human peripheral blood lymphocytes and no erythrocyte hemolysis upon prolonged incubations on matrices indicated good biocompatibility of designed materials. The suitability of photoactive surfaces for PDT was tested in two cell lines relevant to stent implantation: vascular endothelial cells (HUVECs) and vascular smooth muscle cells (VSMC). It was demonstrated that 2 h incubation on the silica matrices was sufficient for uptake of the encapsulated photosensitizers. Moreover, the amount of the absorbed photosensitizer was sufficient for induction of a phototoxic reaction as shown by a rise of the reactive oxygen species in photosensitized VSMC. On the other hand, limited reactive oxygen species (ROS) induction in HUVECs in our experimental set up suggests that the proposed method of PDT may be less harmful for the endothelial cells and may decrease a risk of the restenosis. Presented data clearly demonstrate that porous silica-based matrices are capable of in situ delivery of photosensitizer for PDT of VSMC.
format Online
Article
Text
id pubmed-6947284
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69472842020-01-13 Photoactive Pore Matrix for In Situ Delivery of a Photosensitizer in Vascular Smooth Muscle Cells Selective PDT Wawrzyńska, Magdalena Duda, Maciej Hołowacz, Iwona Kaczorowska, Aleksandra Ulatowska-Jarża, Agnieszka Buzalewicz, Igor Kałas, Wojciech Wysokińska, Edyta Biały, Dariusz Podbielska, Halina Kopaczyńska, Marta Materials (Basel) Article In this study we present the porous silica-based material that can be used for in situ drug delivery, offering effective supply of active compounds regardless its water solubility. To demonstrate usability of this new material, three silica-based materials with different pore size distribution as a matrix for doping with Photolon (Ph) and Protoporphyrin IX (PPIX) photosensitizers, were prepared. These matrices can be used for coating cardiovascular stents used for treatment of the coronary artery disease and enable intravascular photodynamic therapy (PDT), which can modulate the vascular response to injury caused by stent implantation—procedure that should be thought as an alternative for drug eluting stent. The FTIR spectroscopic analysis confirmed that all studied matrices have been successfully functionalized with the target photosensitizers. Atomic force microscopy revealed that resulting photoactive matrices were very smooth, which can limit the implantation damage and reduce the risk of restenosis. No viability loss of human peripheral blood lymphocytes and no erythrocyte hemolysis upon prolonged incubations on matrices indicated good biocompatibility of designed materials. The suitability of photoactive surfaces for PDT was tested in two cell lines relevant to stent implantation: vascular endothelial cells (HUVECs) and vascular smooth muscle cells (VSMC). It was demonstrated that 2 h incubation on the silica matrices was sufficient for uptake of the encapsulated photosensitizers. Moreover, the amount of the absorbed photosensitizer was sufficient for induction of a phototoxic reaction as shown by a rise of the reactive oxygen species in photosensitized VSMC. On the other hand, limited reactive oxygen species (ROS) induction in HUVECs in our experimental set up suggests that the proposed method of PDT may be less harmful for the endothelial cells and may decrease a risk of the restenosis. Presented data clearly demonstrate that porous silica-based matrices are capable of in situ delivery of photosensitizer for PDT of VSMC. MDPI 2019-12-09 /pmc/articles/PMC6947284/ /pubmed/31818025 http://dx.doi.org/10.3390/ma12244110 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wawrzyńska, Magdalena
Duda, Maciej
Hołowacz, Iwona
Kaczorowska, Aleksandra
Ulatowska-Jarża, Agnieszka
Buzalewicz, Igor
Kałas, Wojciech
Wysokińska, Edyta
Biały, Dariusz
Podbielska, Halina
Kopaczyńska, Marta
Photoactive Pore Matrix for In Situ Delivery of a Photosensitizer in Vascular Smooth Muscle Cells Selective PDT
title Photoactive Pore Matrix for In Situ Delivery of a Photosensitizer in Vascular Smooth Muscle Cells Selective PDT
title_full Photoactive Pore Matrix for In Situ Delivery of a Photosensitizer in Vascular Smooth Muscle Cells Selective PDT
title_fullStr Photoactive Pore Matrix for In Situ Delivery of a Photosensitizer in Vascular Smooth Muscle Cells Selective PDT
title_full_unstemmed Photoactive Pore Matrix for In Situ Delivery of a Photosensitizer in Vascular Smooth Muscle Cells Selective PDT
title_short Photoactive Pore Matrix for In Situ Delivery of a Photosensitizer in Vascular Smooth Muscle Cells Selective PDT
title_sort photoactive pore matrix for in situ delivery of a photosensitizer in vascular smooth muscle cells selective pdt
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947284/
https://www.ncbi.nlm.nih.gov/pubmed/31818025
http://dx.doi.org/10.3390/ma12244110
work_keys_str_mv AT wawrzynskamagdalena photoactiveporematrixforinsitudeliveryofaphotosensitizerinvascularsmoothmusclecellsselectivepdt
AT dudamaciej photoactiveporematrixforinsitudeliveryofaphotosensitizerinvascularsmoothmusclecellsselectivepdt
AT hołowacziwona photoactiveporematrixforinsitudeliveryofaphotosensitizerinvascularsmoothmusclecellsselectivepdt
AT kaczorowskaaleksandra photoactiveporematrixforinsitudeliveryofaphotosensitizerinvascularsmoothmusclecellsselectivepdt
AT ulatowskajarzaagnieszka photoactiveporematrixforinsitudeliveryofaphotosensitizerinvascularsmoothmusclecellsselectivepdt
AT buzalewiczigor photoactiveporematrixforinsitudeliveryofaphotosensitizerinvascularsmoothmusclecellsselectivepdt
AT kałaswojciech photoactiveporematrixforinsitudeliveryofaphotosensitizerinvascularsmoothmusclecellsselectivepdt
AT wysokinskaedyta photoactiveporematrixforinsitudeliveryofaphotosensitizerinvascularsmoothmusclecellsselectivepdt
AT białydariusz photoactiveporematrixforinsitudeliveryofaphotosensitizerinvascularsmoothmusclecellsselectivepdt
AT podbielskahalina photoactiveporematrixforinsitudeliveryofaphotosensitizerinvascularsmoothmusclecellsselectivepdt
AT kopaczynskamarta photoactiveporematrixforinsitudeliveryofaphotosensitizerinvascularsmoothmusclecellsselectivepdt