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RNA-Dependent RNA Polymerase Speed and Fidelity are not the Only Determinants of the Mechanism or Efficiency of Recombination

Using the RNA-dependent RNA polymerase (RdRp) from poliovirus (PV) as our model system, we have shown that Lys-359 in motif-D functions as a general acid in the mechanism of nucleotidyl transfer. A K359H (KH) RdRp derivative is slow and faithful relative to wild-type enzyme. In the context of the KH...

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Detalles Bibliográficos
Autores principales:	Kim, Hyejeong, Ellis, Victor D., Woodman, Andrew, Zhao, Yan, Arnold, Jamie J., Cameron, Craig E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947342/ https://www.ncbi.nlm.nih.gov/pubmed/31775299 http://dx.doi.org/10.3390/genes10120968

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