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Ceramide Content in Liver Increases Along with Insulin Resistance in Obese Patients

The liver plays a central role in the glucose and lipid metabolism. Studies performed on animal models have shown an important role of lipid accumulation in the induction of insulin resistance. We sought to explain whether in obese humans, the insulin resistance is associated with hepatic ceramide a...

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Autores principales: Razak Hady, Hady, Błachnio-Zabielska, Agnieszka U., Szczerbiński, Łukasz, Zabielski, Piotr, Imierska, Monika, Dadan, Jacek, Krętowski, Adam J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947381/
https://www.ncbi.nlm.nih.gov/pubmed/31842461
http://dx.doi.org/10.3390/jcm8122197
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author Razak Hady, Hady
Błachnio-Zabielska, Agnieszka U.
Szczerbiński, Łukasz
Zabielski, Piotr
Imierska, Monika
Dadan, Jacek
Krętowski, Adam J.
author_facet Razak Hady, Hady
Błachnio-Zabielska, Agnieszka U.
Szczerbiński, Łukasz
Zabielski, Piotr
Imierska, Monika
Dadan, Jacek
Krętowski, Adam J.
author_sort Razak Hady, Hady
collection PubMed
description The liver plays a central role in the glucose and lipid metabolism. Studies performed on animal models have shown an important role of lipid accumulation in the induction of insulin resistance. We sought to explain whether in obese humans, the insulin resistance is associated with hepatic ceramide accumulation. The experiments were conducted on obese men and women. Each gender was divided into three groups: Normal glucose tolerance group (NGT), Impaired glucose tolerance group (IGT), and Type 2 diabetic subjects (T2D). Ceramide (Cer) content was analyzed with the use of LC/MS/MS. An oral glucose tolerance test (OGTT), glycosylated hemoglobin (HbA1c), percentage body fat (FAT%), and body mass index (BMI) was also measured. Total hepatic ceramide was significantly higher in T2D females as compared to NGT females (p < 0.05), whereas in males, total ceramide was significantly higher in IGT and T2D as compared to NGT (p < 0.05). In both, men and women, the highest increase in T2D subjects, was observed in C16:0-Cer, C18:0:-Cer, C22:0-Cer, and C24:0-Cer (p < 0.05) as compared to NGT group. Interestingly, glucose (at 0′ and at 120′ in OGTT) and HbA1c positively correlated with the ceramide species that most increased in T2D patients (C16:0-Cer, C18:0-Cer, C22:0-Cer, and C24:0-Cer). In men glucose and HbA1c significantly correlated with only C22:0-Cer. This is one of the few studies comparing hepatic ceramide content in severely obese patients. We found that, ceramide content increased in diabetic patients, both in men and women, and the content of ceramide correlated with glycemic parameters. These data indicate ceramide contribution to the induction of hepatic insulin resistance.
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spelling pubmed-69473812020-01-13 Ceramide Content in Liver Increases Along with Insulin Resistance in Obese Patients Razak Hady, Hady Błachnio-Zabielska, Agnieszka U. Szczerbiński, Łukasz Zabielski, Piotr Imierska, Monika Dadan, Jacek Krętowski, Adam J. J Clin Med Article The liver plays a central role in the glucose and lipid metabolism. Studies performed on animal models have shown an important role of lipid accumulation in the induction of insulin resistance. We sought to explain whether in obese humans, the insulin resistance is associated with hepatic ceramide accumulation. The experiments were conducted on obese men and women. Each gender was divided into three groups: Normal glucose tolerance group (NGT), Impaired glucose tolerance group (IGT), and Type 2 diabetic subjects (T2D). Ceramide (Cer) content was analyzed with the use of LC/MS/MS. An oral glucose tolerance test (OGTT), glycosylated hemoglobin (HbA1c), percentage body fat (FAT%), and body mass index (BMI) was also measured. Total hepatic ceramide was significantly higher in T2D females as compared to NGT females (p < 0.05), whereas in males, total ceramide was significantly higher in IGT and T2D as compared to NGT (p < 0.05). In both, men and women, the highest increase in T2D subjects, was observed in C16:0-Cer, C18:0:-Cer, C22:0-Cer, and C24:0-Cer (p < 0.05) as compared to NGT group. Interestingly, glucose (at 0′ and at 120′ in OGTT) and HbA1c positively correlated with the ceramide species that most increased in T2D patients (C16:0-Cer, C18:0-Cer, C22:0-Cer, and C24:0-Cer). In men glucose and HbA1c significantly correlated with only C22:0-Cer. This is one of the few studies comparing hepatic ceramide content in severely obese patients. We found that, ceramide content increased in diabetic patients, both in men and women, and the content of ceramide correlated with glycemic parameters. These data indicate ceramide contribution to the induction of hepatic insulin resistance. MDPI 2019-12-12 /pmc/articles/PMC6947381/ /pubmed/31842461 http://dx.doi.org/10.3390/jcm8122197 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Razak Hady, Hady
Błachnio-Zabielska, Agnieszka U.
Szczerbiński, Łukasz
Zabielski, Piotr
Imierska, Monika
Dadan, Jacek
Krętowski, Adam J.
Ceramide Content in Liver Increases Along with Insulin Resistance in Obese Patients
title Ceramide Content in Liver Increases Along with Insulin Resistance in Obese Patients
title_full Ceramide Content in Liver Increases Along with Insulin Resistance in Obese Patients
title_fullStr Ceramide Content in Liver Increases Along with Insulin Resistance in Obese Patients
title_full_unstemmed Ceramide Content in Liver Increases Along with Insulin Resistance in Obese Patients
title_short Ceramide Content in Liver Increases Along with Insulin Resistance in Obese Patients
title_sort ceramide content in liver increases along with insulin resistance in obese patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947381/
https://www.ncbi.nlm.nih.gov/pubmed/31842461
http://dx.doi.org/10.3390/jcm8122197
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