Cargando…
The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region
The gene family with sequence similarity 13 member A (FAM13A) has recently been identified as a marker gene in insulin sensitivity and lipolysis. In this study, we first analyzed the expression patterns of this gene in different tissues of adult cattle and then constructed a phylogenetic tree based...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947481/ https://www.ncbi.nlm.nih.gov/pubmed/31795267 http://dx.doi.org/10.3390/genes10120981 |
_version_ | 1783485561101615104 |
---|---|
author | Liang, Chengcheng Li, Anning Raza, Sayed Haidar Abbas Khan, Rajwali Wang, Xiaoyu Wang, Sihu Wang, Guohua Zhang, Yu Zan, Linsen |
author_facet | Liang, Chengcheng Li, Anning Raza, Sayed Haidar Abbas Khan, Rajwali Wang, Xiaoyu Wang, Sihu Wang, Guohua Zhang, Yu Zan, Linsen |
author_sort | Liang, Chengcheng |
collection | PubMed |
description | The gene family with sequence similarity 13 member A (FAM13A) has recently been identified as a marker gene in insulin sensitivity and lipolysis. In this study, we first analyzed the expression patterns of this gene in different tissues of adult cattle and then constructed a phylogenetic tree based on the FAM13A amino acid sequence. This showed that subcutaneous adipose tissue had the highest expression in all tissues except lung tissue. Then we summarized the gene structure. The promoter region sequence of the gene was successfully amplified, and the −241/+54 region has been identified as the core promoter region. The core promoter region was determined by the unidirectional deletion of the 5’ flanking promoter region of the FAM13A gene. Based on the bioinformatics analysis, we examined the dual luciferase activity of the vector constructed by the mutation site, and the transcription factors ACSL1 and ASCL2 were found as transcriptional regulators of FAM13A. Moreover, electrophoretic mobility shift assay (EMSA) further validated the regulatory role of ACSL1 and ASCL2 in the regulation of FAM13A. ACSL1 and ASCL2 were finally identified as activating transcription factors. Our results provide a basis for the function of the FAM13A gene in bovine adipocytes in order to improve the deposition of fat deposition in beef cattle muscle. |
format | Online Article Text |
id | pubmed-6947481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69474812020-01-13 The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region Liang, Chengcheng Li, Anning Raza, Sayed Haidar Abbas Khan, Rajwali Wang, Xiaoyu Wang, Sihu Wang, Guohua Zhang, Yu Zan, Linsen Genes (Basel) Article The gene family with sequence similarity 13 member A (FAM13A) has recently been identified as a marker gene in insulin sensitivity and lipolysis. In this study, we first analyzed the expression patterns of this gene in different tissues of adult cattle and then constructed a phylogenetic tree based on the FAM13A amino acid sequence. This showed that subcutaneous adipose tissue had the highest expression in all tissues except lung tissue. Then we summarized the gene structure. The promoter region sequence of the gene was successfully amplified, and the −241/+54 region has been identified as the core promoter region. The core promoter region was determined by the unidirectional deletion of the 5’ flanking promoter region of the FAM13A gene. Based on the bioinformatics analysis, we examined the dual luciferase activity of the vector constructed by the mutation site, and the transcription factors ACSL1 and ASCL2 were found as transcriptional regulators of FAM13A. Moreover, electrophoretic mobility shift assay (EMSA) further validated the regulatory role of ACSL1 and ASCL2 in the regulation of FAM13A. ACSL1 and ASCL2 were finally identified as activating transcription factors. Our results provide a basis for the function of the FAM13A gene in bovine adipocytes in order to improve the deposition of fat deposition in beef cattle muscle. MDPI 2019-11-28 /pmc/articles/PMC6947481/ /pubmed/31795267 http://dx.doi.org/10.3390/genes10120981 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liang, Chengcheng Li, Anning Raza, Sayed Haidar Abbas Khan, Rajwali Wang, Xiaoyu Wang, Sihu Wang, Guohua Zhang, Yu Zan, Linsen The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region |
title | The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region |
title_full | The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region |
title_fullStr | The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region |
title_full_unstemmed | The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region |
title_short | The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region |
title_sort | molecular characteristics of the fam13a gene and the role of transcription factors acsl1 and ascl2 in its core promoter region |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947481/ https://www.ncbi.nlm.nih.gov/pubmed/31795267 http://dx.doi.org/10.3390/genes10120981 |
work_keys_str_mv | AT liangchengcheng themolecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT lianning themolecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT razasayedhaidarabbas themolecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT khanrajwali themolecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT wangxiaoyu themolecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT wangsihu themolecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT wangguohua themolecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT zhangyu themolecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT zanlinsen themolecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT liangchengcheng molecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT lianning molecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT razasayedhaidarabbas molecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT khanrajwali molecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT wangxiaoyu molecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT wangsihu molecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT wangguohua molecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT zhangyu molecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion AT zanlinsen molecularcharacteristicsofthefam13ageneandtheroleoftranscriptionfactorsacsl1andascl2initscorepromoterregion |