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The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region

The gene family with sequence similarity 13 member A (FAM13A) has recently been identified as a marker gene in insulin sensitivity and lipolysis. In this study, we first analyzed the expression patterns of this gene in different tissues of adult cattle and then constructed a phylogenetic tree based...

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Autores principales: Liang, Chengcheng, Li, Anning, Raza, Sayed Haidar Abbas, Khan, Rajwali, Wang, Xiaoyu, Wang, Sihu, Wang, Guohua, Zhang, Yu, Zan, Linsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947481/
https://www.ncbi.nlm.nih.gov/pubmed/31795267
http://dx.doi.org/10.3390/genes10120981
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author Liang, Chengcheng
Li, Anning
Raza, Sayed Haidar Abbas
Khan, Rajwali
Wang, Xiaoyu
Wang, Sihu
Wang, Guohua
Zhang, Yu
Zan, Linsen
author_facet Liang, Chengcheng
Li, Anning
Raza, Sayed Haidar Abbas
Khan, Rajwali
Wang, Xiaoyu
Wang, Sihu
Wang, Guohua
Zhang, Yu
Zan, Linsen
author_sort Liang, Chengcheng
collection PubMed
description The gene family with sequence similarity 13 member A (FAM13A) has recently been identified as a marker gene in insulin sensitivity and lipolysis. In this study, we first analyzed the expression patterns of this gene in different tissues of adult cattle and then constructed a phylogenetic tree based on the FAM13A amino acid sequence. This showed that subcutaneous adipose tissue had the highest expression in all tissues except lung tissue. Then we summarized the gene structure. The promoter region sequence of the gene was successfully amplified, and the −241/+54 region has been identified as the core promoter region. The core promoter region was determined by the unidirectional deletion of the 5’ flanking promoter region of the FAM13A gene. Based on the bioinformatics analysis, we examined the dual luciferase activity of the vector constructed by the mutation site, and the transcription factors ACSL1 and ASCL2 were found as transcriptional regulators of FAM13A. Moreover, electrophoretic mobility shift assay (EMSA) further validated the regulatory role of ACSL1 and ASCL2 in the regulation of FAM13A. ACSL1 and ASCL2 were finally identified as activating transcription factors. Our results provide a basis for the function of the FAM13A gene in bovine adipocytes in order to improve the deposition of fat deposition in beef cattle muscle.
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spelling pubmed-69474812020-01-13 The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region Liang, Chengcheng Li, Anning Raza, Sayed Haidar Abbas Khan, Rajwali Wang, Xiaoyu Wang, Sihu Wang, Guohua Zhang, Yu Zan, Linsen Genes (Basel) Article The gene family with sequence similarity 13 member A (FAM13A) has recently been identified as a marker gene in insulin sensitivity and lipolysis. In this study, we first analyzed the expression patterns of this gene in different tissues of adult cattle and then constructed a phylogenetic tree based on the FAM13A amino acid sequence. This showed that subcutaneous adipose tissue had the highest expression in all tissues except lung tissue. Then we summarized the gene structure. The promoter region sequence of the gene was successfully amplified, and the −241/+54 region has been identified as the core promoter region. The core promoter region was determined by the unidirectional deletion of the 5’ flanking promoter region of the FAM13A gene. Based on the bioinformatics analysis, we examined the dual luciferase activity of the vector constructed by the mutation site, and the transcription factors ACSL1 and ASCL2 were found as transcriptional regulators of FAM13A. Moreover, electrophoretic mobility shift assay (EMSA) further validated the regulatory role of ACSL1 and ASCL2 in the regulation of FAM13A. ACSL1 and ASCL2 were finally identified as activating transcription factors. Our results provide a basis for the function of the FAM13A gene in bovine adipocytes in order to improve the deposition of fat deposition in beef cattle muscle. MDPI 2019-11-28 /pmc/articles/PMC6947481/ /pubmed/31795267 http://dx.doi.org/10.3390/genes10120981 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liang, Chengcheng
Li, Anning
Raza, Sayed Haidar Abbas
Khan, Rajwali
Wang, Xiaoyu
Wang, Sihu
Wang, Guohua
Zhang, Yu
Zan, Linsen
The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region
title The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region
title_full The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region
title_fullStr The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region
title_full_unstemmed The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region
title_short The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region
title_sort molecular characteristics of the fam13a gene and the role of transcription factors acsl1 and ascl2 in its core promoter region
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947481/
https://www.ncbi.nlm.nih.gov/pubmed/31795267
http://dx.doi.org/10.3390/genes10120981
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