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Variants in Miro1 Cause Alterations of ER-Mitochondria Contact Sites in Fibroblasts from Parkinson’s Disease Patients

Background: Although most cases of Parkinson´s disease (PD) are idiopathic with unknown cause, an increasing number of genes and genetic risk factors have been discovered that play a role in PD pathogenesis. Many of the PD-associated proteins are involved in mitochondrial quality control, e.g., PINK...

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Autores principales: Berenguer-Escuder, Clara, Grossmann, Dajana, Massart, Franҫois, Antony, Paul, Burbulla, Lena F., Glaab, Enrico, Imhoff, Sophie, Trinh, Joanne, Seibler, Philip, Grünewald, Anne, Krüger, Rejko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947516/
https://www.ncbi.nlm.nih.gov/pubmed/31888276
http://dx.doi.org/10.3390/jcm8122226
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author Berenguer-Escuder, Clara
Grossmann, Dajana
Massart, Franҫois
Antony, Paul
Burbulla, Lena F.
Glaab, Enrico
Imhoff, Sophie
Trinh, Joanne
Seibler, Philip
Grünewald, Anne
Krüger, Rejko
author_facet Berenguer-Escuder, Clara
Grossmann, Dajana
Massart, Franҫois
Antony, Paul
Burbulla, Lena F.
Glaab, Enrico
Imhoff, Sophie
Trinh, Joanne
Seibler, Philip
Grünewald, Anne
Krüger, Rejko
author_sort Berenguer-Escuder, Clara
collection PubMed
description Background: Although most cases of Parkinson´s disease (PD) are idiopathic with unknown cause, an increasing number of genes and genetic risk factors have been discovered that play a role in PD pathogenesis. Many of the PD-associated proteins are involved in mitochondrial quality control, e.g., PINK1, Parkin, and LRRK2, which were recently identified as regulators of mitochondrial-endoplasmic reticulum (ER) contact sites (MERCs) linking mitochondrial homeostasis to intracellular calcium handling. In this context, Miro1 is increasingly recognized to play a role in PD pathology. Recently, we identified the first PD patients carrying mutations in RHOT1, the gene coding for Miro1. Here, we describe two novel RHOT1 mutations identified in two PD patients and the characterization of the cellular phenotypes. Methods: Using whole exome sequencing we identified two PD patients carrying heterozygous mutations leading to the amino acid exchanges T351A and T610A in Miro1. We analyzed calcium homeostasis and MERCs in detail by live cell imaging and immunocytochemistry in patient-derived fibroblasts. Results: We show that fibroblasts expressing mutant T351A or T610A Miro1 display impaired calcium homeostasis and a reduced amount of MERCs. All fibroblast lines from patients with pathogenic variants in Miro1, revealed alterations of the structure of MERCs. Conclusion: Our data suggest that Miro1 is important for the regulation of the structure and function of MERCs. Moreover, our study supports the role of MERCs in the pathogenesis of PD and further establishes variants in RHOT1 as rare genetic risk factors for neurodegeneration.
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spelling pubmed-69475162020-01-13 Variants in Miro1 Cause Alterations of ER-Mitochondria Contact Sites in Fibroblasts from Parkinson’s Disease Patients Berenguer-Escuder, Clara Grossmann, Dajana Massart, Franҫois Antony, Paul Burbulla, Lena F. Glaab, Enrico Imhoff, Sophie Trinh, Joanne Seibler, Philip Grünewald, Anne Krüger, Rejko J Clin Med Article Background: Although most cases of Parkinson´s disease (PD) are idiopathic with unknown cause, an increasing number of genes and genetic risk factors have been discovered that play a role in PD pathogenesis. Many of the PD-associated proteins are involved in mitochondrial quality control, e.g., PINK1, Parkin, and LRRK2, which were recently identified as regulators of mitochondrial-endoplasmic reticulum (ER) contact sites (MERCs) linking mitochondrial homeostasis to intracellular calcium handling. In this context, Miro1 is increasingly recognized to play a role in PD pathology. Recently, we identified the first PD patients carrying mutations in RHOT1, the gene coding for Miro1. Here, we describe two novel RHOT1 mutations identified in two PD patients and the characterization of the cellular phenotypes. Methods: Using whole exome sequencing we identified two PD patients carrying heterozygous mutations leading to the amino acid exchanges T351A and T610A in Miro1. We analyzed calcium homeostasis and MERCs in detail by live cell imaging and immunocytochemistry in patient-derived fibroblasts. Results: We show that fibroblasts expressing mutant T351A or T610A Miro1 display impaired calcium homeostasis and a reduced amount of MERCs. All fibroblast lines from patients with pathogenic variants in Miro1, revealed alterations of the structure of MERCs. Conclusion: Our data suggest that Miro1 is important for the regulation of the structure and function of MERCs. Moreover, our study supports the role of MERCs in the pathogenesis of PD and further establishes variants in RHOT1 as rare genetic risk factors for neurodegeneration. MDPI 2019-12-16 /pmc/articles/PMC6947516/ /pubmed/31888276 http://dx.doi.org/10.3390/jcm8122226 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Berenguer-Escuder, Clara
Grossmann, Dajana
Massart, Franҫois
Antony, Paul
Burbulla, Lena F.
Glaab, Enrico
Imhoff, Sophie
Trinh, Joanne
Seibler, Philip
Grünewald, Anne
Krüger, Rejko
Variants in Miro1 Cause Alterations of ER-Mitochondria Contact Sites in Fibroblasts from Parkinson’s Disease Patients
title Variants in Miro1 Cause Alterations of ER-Mitochondria Contact Sites in Fibroblasts from Parkinson’s Disease Patients
title_full Variants in Miro1 Cause Alterations of ER-Mitochondria Contact Sites in Fibroblasts from Parkinson’s Disease Patients
title_fullStr Variants in Miro1 Cause Alterations of ER-Mitochondria Contact Sites in Fibroblasts from Parkinson’s Disease Patients
title_full_unstemmed Variants in Miro1 Cause Alterations of ER-Mitochondria Contact Sites in Fibroblasts from Parkinson’s Disease Patients
title_short Variants in Miro1 Cause Alterations of ER-Mitochondria Contact Sites in Fibroblasts from Parkinson’s Disease Patients
title_sort variants in miro1 cause alterations of er-mitochondria contact sites in fibroblasts from parkinson’s disease patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947516/
https://www.ncbi.nlm.nih.gov/pubmed/31888276
http://dx.doi.org/10.3390/jcm8122226
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