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Rhizobia Isolated from the Relict Legume Vavilovia formosa Represent a Genetically Specific Group within Rhizobium leguminosarum biovar viciae

Twenty-two rhizobia strains isolated from three distinct populations (North Ossetia, Dagestan, and Armenia) of a relict legume Vavilovia formosa were analysed to determine their position within Rhizobium leguminosarum biovar viciae (Rlv). These bacteria are described as symbionts of four plant gener...

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Autores principales: Kimeklis, Anastasiia K., Chirak, Elizaveta R., Kuznetsova, Irina G., Sazanova, Anna L., Safronova, Vera I., Belimov, Andrey A., Onishchuk, Olga P., Kurchak, Oksana N., Aksenova, Tatyana S., Pinaev, Alexander G., Andronov, Evgeny E., Provorov, Nikolay A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947851/
https://www.ncbi.nlm.nih.gov/pubmed/31805683
http://dx.doi.org/10.3390/genes10120991
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author Kimeklis, Anastasiia K.
Chirak, Elizaveta R.
Kuznetsova, Irina G.
Sazanova, Anna L.
Safronova, Vera I.
Belimov, Andrey A.
Onishchuk, Olga P.
Kurchak, Oksana N.
Aksenova, Tatyana S.
Pinaev, Alexander G.
Andronov, Evgeny E.
Provorov, Nikolay A.
author_facet Kimeklis, Anastasiia K.
Chirak, Elizaveta R.
Kuznetsova, Irina G.
Sazanova, Anna L.
Safronova, Vera I.
Belimov, Andrey A.
Onishchuk, Olga P.
Kurchak, Oksana N.
Aksenova, Tatyana S.
Pinaev, Alexander G.
Andronov, Evgeny E.
Provorov, Nikolay A.
author_sort Kimeklis, Anastasiia K.
collection PubMed
description Twenty-two rhizobia strains isolated from three distinct populations (North Ossetia, Dagestan, and Armenia) of a relict legume Vavilovia formosa were analysed to determine their position within Rhizobium leguminosarum biovar viciae (Rlv). These bacteria are described as symbionts of four plant genera Pisum, Vicia, Lathyrus, and Lens from the Fabeae tribe, of which Vavilovia is considered to be closest to its last common ancestor (LCA). In contrast to biovar viciae, bacteria from Rhizobium leguminosarum biovar trifolii (Rlt) inoculate plants from the Trifolieae tribe. Comparison of house-keeping (hkg: 16S rRNA, glnII, gltA, and dnaK) and symbiotic (sym: nodA, nodC, nodD, and nifH) genes of the symbionts of V. formosa with those of other Rlv and Rlt strains reveals a significant group separation, which was most pronounced for sym genes. A remarkable feature of the strains isolated from V. formosa was the presence of the nodX gene, which was commonly found in Rlv strains isolated from Afghanistan pea genotypes. Tube testing of different strains on nine plant species, including all genera from the Fabeae tribe, demonstrated that the strains from V. formosa nodulated the same cross inoculation group as the other Rlv strains. Comparison of nucleotide similarity in sym genes suggested that their diversification within sym-biotypes of Rlv was elicited by host plants. Contrariwise, that of hkg genes could be caused by either local adaptation to soil niches or by genetic drift. Long-term ecological isolation, genetic separation, and the ancestral position of V. formosa suggested that symbionts of V. formosa could be responsible for preserving ancestral genotypes of the Rlv biovar.
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spelling pubmed-69478512020-01-13 Rhizobia Isolated from the Relict Legume Vavilovia formosa Represent a Genetically Specific Group within Rhizobium leguminosarum biovar viciae Kimeklis, Anastasiia K. Chirak, Elizaveta R. Kuznetsova, Irina G. Sazanova, Anna L. Safronova, Vera I. Belimov, Andrey A. Onishchuk, Olga P. Kurchak, Oksana N. Aksenova, Tatyana S. Pinaev, Alexander G. Andronov, Evgeny E. Provorov, Nikolay A. Genes (Basel) Article Twenty-two rhizobia strains isolated from three distinct populations (North Ossetia, Dagestan, and Armenia) of a relict legume Vavilovia formosa were analysed to determine their position within Rhizobium leguminosarum biovar viciae (Rlv). These bacteria are described as symbionts of four plant genera Pisum, Vicia, Lathyrus, and Lens from the Fabeae tribe, of which Vavilovia is considered to be closest to its last common ancestor (LCA). In contrast to biovar viciae, bacteria from Rhizobium leguminosarum biovar trifolii (Rlt) inoculate plants from the Trifolieae tribe. Comparison of house-keeping (hkg: 16S rRNA, glnII, gltA, and dnaK) and symbiotic (sym: nodA, nodC, nodD, and nifH) genes of the symbionts of V. formosa with those of other Rlv and Rlt strains reveals a significant group separation, which was most pronounced for sym genes. A remarkable feature of the strains isolated from V. formosa was the presence of the nodX gene, which was commonly found in Rlv strains isolated from Afghanistan pea genotypes. Tube testing of different strains on nine plant species, including all genera from the Fabeae tribe, demonstrated that the strains from V. formosa nodulated the same cross inoculation group as the other Rlv strains. Comparison of nucleotide similarity in sym genes suggested that their diversification within sym-biotypes of Rlv was elicited by host plants. Contrariwise, that of hkg genes could be caused by either local adaptation to soil niches or by genetic drift. Long-term ecological isolation, genetic separation, and the ancestral position of V. formosa suggested that symbionts of V. formosa could be responsible for preserving ancestral genotypes of the Rlv biovar. MDPI 2019-12-01 /pmc/articles/PMC6947851/ /pubmed/31805683 http://dx.doi.org/10.3390/genes10120991 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kimeklis, Anastasiia K.
Chirak, Elizaveta R.
Kuznetsova, Irina G.
Sazanova, Anna L.
Safronova, Vera I.
Belimov, Andrey A.
Onishchuk, Olga P.
Kurchak, Oksana N.
Aksenova, Tatyana S.
Pinaev, Alexander G.
Andronov, Evgeny E.
Provorov, Nikolay A.
Rhizobia Isolated from the Relict Legume Vavilovia formosa Represent a Genetically Specific Group within Rhizobium leguminosarum biovar viciae
title Rhizobia Isolated from the Relict Legume Vavilovia formosa Represent a Genetically Specific Group within Rhizobium leguminosarum biovar viciae
title_full Rhizobia Isolated from the Relict Legume Vavilovia formosa Represent a Genetically Specific Group within Rhizobium leguminosarum biovar viciae
title_fullStr Rhizobia Isolated from the Relict Legume Vavilovia formosa Represent a Genetically Specific Group within Rhizobium leguminosarum biovar viciae
title_full_unstemmed Rhizobia Isolated from the Relict Legume Vavilovia formosa Represent a Genetically Specific Group within Rhizobium leguminosarum biovar viciae
title_short Rhizobia Isolated from the Relict Legume Vavilovia formosa Represent a Genetically Specific Group within Rhizobium leguminosarum biovar viciae
title_sort rhizobia isolated from the relict legume vavilovia formosa represent a genetically specific group within rhizobium leguminosarum biovar viciae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947851/
https://www.ncbi.nlm.nih.gov/pubmed/31805683
http://dx.doi.org/10.3390/genes10120991
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