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The micronucleus assay in the oral exfoliated cells of tannery workers
CONTEXT: Micronuclei, tannery workers, chromium, papanicolaou (PAP) stain. AIMS: The presence of micronuclei are biomarkers broadly used; the detection of micronuclei offers a great opportunity to monitor individuals or populations exposed to mutagenic, genotoxic or teratogenic events, mainly the ev...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948052/ https://www.ncbi.nlm.nih.gov/pubmed/31942137 http://dx.doi.org/10.4103/jomfp.JOMFP_321_18 |
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author | Kalim, Mohd Shadab Khare, Abhisheik Shreedhar, Balasundari Hadari, Safiya Gupta, Vivek Mahendra, Anupama |
author_facet | Kalim, Mohd Shadab Khare, Abhisheik Shreedhar, Balasundari Hadari, Safiya Gupta, Vivek Mahendra, Anupama |
author_sort | Kalim, Mohd Shadab |
collection | PubMed |
description | CONTEXT: Micronuclei, tannery workers, chromium, papanicolaou (PAP) stain. AIMS: The presence of micronuclei are biomarkers broadly used; the detection of micronuclei offers a great opportunity to monitor individuals or populations exposed to mutagenic, genotoxic or teratogenic events, mainly the evaluation of micronucleogenic cells presence in epithelial tissues. SETTINGS AND DESIGN: Tannery workers with and without tobacco usage was considered for microneuceli assessment using PAP stain, 50 patients without usage of tobacco was taken and 50 patients with usage of tobacco was considered. SUBJECTS AND METHODS: Cytological smears were produced and stained with PAP stain for the evaluation of micronuclei. STATISTICAL ANALYSIS USED: Students t-test, analysis of variance. RESULTS: Our findings concluded that chromium exposure causes instability of the genetic material in the tannery workers and can be taken as an indication that these individuals have increased cancer risks. The present study also concluded that PAP could be used as a specific marker for micronuclei assessment. CONCLUSIONS: In our case study, we have seen increasing number of micronuclei frequencies in tobacco user's tannery workers than nonuser's tannery workers, which we can use as a predictor marker of premalignant and malignant lesion. |
format | Online Article Text |
id | pubmed-6948052 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-69480522020-01-15 The micronucleus assay in the oral exfoliated cells of tannery workers Kalim, Mohd Shadab Khare, Abhisheik Shreedhar, Balasundari Hadari, Safiya Gupta, Vivek Mahendra, Anupama J Oral Maxillofac Pathol Original Article CONTEXT: Micronuclei, tannery workers, chromium, papanicolaou (PAP) stain. AIMS: The presence of micronuclei are biomarkers broadly used; the detection of micronuclei offers a great opportunity to monitor individuals or populations exposed to mutagenic, genotoxic or teratogenic events, mainly the evaluation of micronucleogenic cells presence in epithelial tissues. SETTINGS AND DESIGN: Tannery workers with and without tobacco usage was considered for microneuceli assessment using PAP stain, 50 patients without usage of tobacco was taken and 50 patients with usage of tobacco was considered. SUBJECTS AND METHODS: Cytological smears were produced and stained with PAP stain for the evaluation of micronuclei. STATISTICAL ANALYSIS USED: Students t-test, analysis of variance. RESULTS: Our findings concluded that chromium exposure causes instability of the genetic material in the tannery workers and can be taken as an indication that these individuals have increased cancer risks. The present study also concluded that PAP could be used as a specific marker for micronuclei assessment. CONCLUSIONS: In our case study, we have seen increasing number of micronuclei frequencies in tobacco user's tannery workers than nonuser's tannery workers, which we can use as a predictor marker of premalignant and malignant lesion. Wolters Kluwer - Medknow 2019 /pmc/articles/PMC6948052/ /pubmed/31942137 http://dx.doi.org/10.4103/jomfp.JOMFP_321_18 Text en Copyright: © 2019 Journal of Oral and Maxillofacial Pathology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Kalim, Mohd Shadab Khare, Abhisheik Shreedhar, Balasundari Hadari, Safiya Gupta, Vivek Mahendra, Anupama The micronucleus assay in the oral exfoliated cells of tannery workers |
title | The micronucleus assay in the oral exfoliated cells of tannery workers |
title_full | The micronucleus assay in the oral exfoliated cells of tannery workers |
title_fullStr | The micronucleus assay in the oral exfoliated cells of tannery workers |
title_full_unstemmed | The micronucleus assay in the oral exfoliated cells of tannery workers |
title_short | The micronucleus assay in the oral exfoliated cells of tannery workers |
title_sort | micronucleus assay in the oral exfoliated cells of tannery workers |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948052/ https://www.ncbi.nlm.nih.gov/pubmed/31942137 http://dx.doi.org/10.4103/jomfp.JOMFP_321_18 |
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