Cargando…
Identification of Mitochondrial Ligands with Hepatoprotective Activity from Notopterygii Rhizoma et Radix Using Affinity Ultrafiltration/Liquid Chromatography/Mass Spectrometry
In recent years, the incidence of diseases associated with hepatic injury has increased in prevalence. Targeting the mitochondria to protect liver function has gained momentum due to their central role in energy production, apoptotic cell death, oxidative stress, calcium homeostasis, and lipid metab...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948297/ https://www.ncbi.nlm.nih.gov/pubmed/31950043 http://dx.doi.org/10.1155/2019/5729263 |
_version_ | 1783485717945516032 |
---|---|
author | Liang, Li Li, Feng-Jiao Liu, Xin Mu, Jian-Kang Wang, Xi Dong, Jin-Cai Zeng, Lin-Xi Gu, Wen Li, Jing-Ping Yang, Xing-Xin Yu, Jie |
author_facet | Liang, Li Li, Feng-Jiao Liu, Xin Mu, Jian-Kang Wang, Xi Dong, Jin-Cai Zeng, Lin-Xi Gu, Wen Li, Jing-Ping Yang, Xing-Xin Yu, Jie |
author_sort | Liang, Li |
collection | PubMed |
description | In recent years, the incidence of diseases associated with hepatic injury has increased in prevalence. Targeting the mitochondria to protect liver function has gained momentum due to their central role in energy production, apoptotic cell death, oxidative stress, calcium homeostasis, and lipid metabolism. In this study, we employed a hepatic mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method (CM-HMC) to identify hepatic mitochondria ligands from medicinal herbs (MHs) including Notopterygii Rhizoma et Radix (NRR) that possess hepatic-protective effects. A total of 4 newly identified mitochondrial ligands were successfully identified by CM-HMC. The mitochondria-regulating activities of 3 of the 4 hits were confirmed using isolated mitochondria. The hepatic-protective effects of one of these hits were validated in carbon tetrachloride-damaged human liver L02 cell models. We have thus identified new natural hepatic-protectants that enhance our understanding of the hepatic-protective mechanisms of MHs. CM-HMC was proven to efficiently screen for mitochondrial ligands from MHs. |
format | Online Article Text |
id | pubmed-6948297 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-69482972020-01-16 Identification of Mitochondrial Ligands with Hepatoprotective Activity from Notopterygii Rhizoma et Radix Using Affinity Ultrafiltration/Liquid Chromatography/Mass Spectrometry Liang, Li Li, Feng-Jiao Liu, Xin Mu, Jian-Kang Wang, Xi Dong, Jin-Cai Zeng, Lin-Xi Gu, Wen Li, Jing-Ping Yang, Xing-Xin Yu, Jie Biomed Res Int Research Article In recent years, the incidence of diseases associated with hepatic injury has increased in prevalence. Targeting the mitochondria to protect liver function has gained momentum due to their central role in energy production, apoptotic cell death, oxidative stress, calcium homeostasis, and lipid metabolism. In this study, we employed a hepatic mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method (CM-HMC) to identify hepatic mitochondria ligands from medicinal herbs (MHs) including Notopterygii Rhizoma et Radix (NRR) that possess hepatic-protective effects. A total of 4 newly identified mitochondrial ligands were successfully identified by CM-HMC. The mitochondria-regulating activities of 3 of the 4 hits were confirmed using isolated mitochondria. The hepatic-protective effects of one of these hits were validated in carbon tetrachloride-damaged human liver L02 cell models. We have thus identified new natural hepatic-protectants that enhance our understanding of the hepatic-protective mechanisms of MHs. CM-HMC was proven to efficiently screen for mitochondrial ligands from MHs. Hindawi 2019-12-16 /pmc/articles/PMC6948297/ /pubmed/31950043 http://dx.doi.org/10.1155/2019/5729263 Text en Copyright © 2019 Li Liang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liang, Li Li, Feng-Jiao Liu, Xin Mu, Jian-Kang Wang, Xi Dong, Jin-Cai Zeng, Lin-Xi Gu, Wen Li, Jing-Ping Yang, Xing-Xin Yu, Jie Identification of Mitochondrial Ligands with Hepatoprotective Activity from Notopterygii Rhizoma et Radix Using Affinity Ultrafiltration/Liquid Chromatography/Mass Spectrometry |
title | Identification of Mitochondrial Ligands with Hepatoprotective Activity from Notopterygii Rhizoma et Radix Using Affinity Ultrafiltration/Liquid Chromatography/Mass Spectrometry |
title_full | Identification of Mitochondrial Ligands with Hepatoprotective Activity from Notopterygii Rhizoma et Radix Using Affinity Ultrafiltration/Liquid Chromatography/Mass Spectrometry |
title_fullStr | Identification of Mitochondrial Ligands with Hepatoprotective Activity from Notopterygii Rhizoma et Radix Using Affinity Ultrafiltration/Liquid Chromatography/Mass Spectrometry |
title_full_unstemmed | Identification of Mitochondrial Ligands with Hepatoprotective Activity from Notopterygii Rhizoma et Radix Using Affinity Ultrafiltration/Liquid Chromatography/Mass Spectrometry |
title_short | Identification of Mitochondrial Ligands with Hepatoprotective Activity from Notopterygii Rhizoma et Radix Using Affinity Ultrafiltration/Liquid Chromatography/Mass Spectrometry |
title_sort | identification of mitochondrial ligands with hepatoprotective activity from notopterygii rhizoma et radix using affinity ultrafiltration/liquid chromatography/mass spectrometry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948297/ https://www.ncbi.nlm.nih.gov/pubmed/31950043 http://dx.doi.org/10.1155/2019/5729263 |
work_keys_str_mv | AT liangli identificationofmitochondrialligandswithhepatoprotectiveactivityfromnotopterygiirhizomaetradixusingaffinityultrafiltrationliquidchromatographymassspectrometry AT lifengjiao identificationofmitochondrialligandswithhepatoprotectiveactivityfromnotopterygiirhizomaetradixusingaffinityultrafiltrationliquidchromatographymassspectrometry AT liuxin identificationofmitochondrialligandswithhepatoprotectiveactivityfromnotopterygiirhizomaetradixusingaffinityultrafiltrationliquidchromatographymassspectrometry AT mujiankang identificationofmitochondrialligandswithhepatoprotectiveactivityfromnotopterygiirhizomaetradixusingaffinityultrafiltrationliquidchromatographymassspectrometry AT wangxi identificationofmitochondrialligandswithhepatoprotectiveactivityfromnotopterygiirhizomaetradixusingaffinityultrafiltrationliquidchromatographymassspectrometry AT dongjincai identificationofmitochondrialligandswithhepatoprotectiveactivityfromnotopterygiirhizomaetradixusingaffinityultrafiltrationliquidchromatographymassspectrometry AT zenglinxi identificationofmitochondrialligandswithhepatoprotectiveactivityfromnotopterygiirhizomaetradixusingaffinityultrafiltrationliquidchromatographymassspectrometry AT guwen identificationofmitochondrialligandswithhepatoprotectiveactivityfromnotopterygiirhizomaetradixusingaffinityultrafiltrationliquidchromatographymassspectrometry AT lijingping identificationofmitochondrialligandswithhepatoprotectiveactivityfromnotopterygiirhizomaetradixusingaffinityultrafiltrationliquidchromatographymassspectrometry AT yangxingxin identificationofmitochondrialligandswithhepatoprotectiveactivityfromnotopterygiirhizomaetradixusingaffinityultrafiltrationliquidchromatographymassspectrometry AT yujie identificationofmitochondrialligandswithhepatoprotectiveactivityfromnotopterygiirhizomaetradixusingaffinityultrafiltrationliquidchromatographymassspectrometry |