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Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil

The chikungunya East/Central/South/Africa virus lineage (CHIKV-ECSA) was first detected in Brazil in the municipality of Feira de Santana (FS) by mid 2014. Following that, a large number of CHIKV cases have been notified in FS, which is the second-most populous city in Bahia state, northeastern Braz...

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Autores principales: Goes de Jesus, Jaqueline, da Luz Wallau, Gabriel, Lima Maia, Maricelia, Xavier, Joilson, Oliveira Lima, Maria Aparecida, Fonseca, Vagner, Salgado de Abreu, Alvaro, Fraga de Oliveira Tosta, Stephane, Ramos do Amaral, Helineide, Andrade Barbosa Lima, Italo, Viana Silva, Paloma, Carlos dos Santos, Daiana, Sousa de Oliveira, Aline, Campos de Souza, Siane, Barreto Falcão, Melissa, Cerqueira, Erenilde, Ceschini Machado, Laís, Sobral, Mariana Carolina, Teodoro Rezende, Tatiana Maria, Ribeiro Pereira, Mylena, Mota Pereira, Felicidade, Pereira Gusmão Maia, Zuinara, Freitas de Oliveira França, Rafael, Luiz de Abreu, André, Campelo de Albuquerque e Melo, Carlos Frederico, Rodrigues Faria, Nuno, Venâncio da Cunha, Rivaldo, Giovanetti, Marta, Alcantara, Luiz Carlos Junior
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948741/
https://www.ncbi.nlm.nih.gov/pubmed/31914137
http://dx.doi.org/10.1371/journal.pone.0226098
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author Goes de Jesus, Jaqueline
da Luz Wallau, Gabriel
Lima Maia, Maricelia
Xavier, Joilson
Oliveira Lima, Maria Aparecida
Fonseca, Vagner
Salgado de Abreu, Alvaro
Fraga de Oliveira Tosta, Stephane
Ramos do Amaral, Helineide
Andrade Barbosa Lima, Italo
Viana Silva, Paloma
Carlos dos Santos, Daiana
Sousa de Oliveira, Aline
Campos de Souza, Siane
Barreto Falcão, Melissa
Cerqueira, Erenilde
Ceschini Machado, Laís
Sobral, Mariana Carolina
Teodoro Rezende, Tatiana Maria
Ribeiro Pereira, Mylena
Mota Pereira, Felicidade
Pereira Gusmão Maia, Zuinara
Freitas de Oliveira França, Rafael
Luiz de Abreu, André
Campelo de Albuquerque e Melo, Carlos Frederico
Rodrigues Faria, Nuno
Venâncio da Cunha, Rivaldo
Giovanetti, Marta
Alcantara, Luiz Carlos Junior
author_facet Goes de Jesus, Jaqueline
da Luz Wallau, Gabriel
Lima Maia, Maricelia
Xavier, Joilson
Oliveira Lima, Maria Aparecida
Fonseca, Vagner
Salgado de Abreu, Alvaro
Fraga de Oliveira Tosta, Stephane
Ramos do Amaral, Helineide
Andrade Barbosa Lima, Italo
Viana Silva, Paloma
Carlos dos Santos, Daiana
Sousa de Oliveira, Aline
Campos de Souza, Siane
Barreto Falcão, Melissa
Cerqueira, Erenilde
Ceschini Machado, Laís
Sobral, Mariana Carolina
Teodoro Rezende, Tatiana Maria
Ribeiro Pereira, Mylena
Mota Pereira, Felicidade
Pereira Gusmão Maia, Zuinara
Freitas de Oliveira França, Rafael
Luiz de Abreu, André
Campelo de Albuquerque e Melo, Carlos Frederico
Rodrigues Faria, Nuno
Venâncio da Cunha, Rivaldo
Giovanetti, Marta
Alcantara, Luiz Carlos Junior
author_sort Goes de Jesus, Jaqueline
collection PubMed
description The chikungunya East/Central/South/Africa virus lineage (CHIKV-ECSA) was first detected in Brazil in the municipality of Feira de Santana (FS) by mid 2014. Following that, a large number of CHIKV cases have been notified in FS, which is the second-most populous city in Bahia state, northeastern Brazil, and plays an important role on the spread to other Brazilian states due to climate conditions and the abundance of competent vectors. To better understand CHIKV dynamics in Bahia state, we generated 5 complete genome sequences from a local outbreak raised in Serraria Brasil, a neighbourhood in FS, by next-generation sequencing using Illumina approach. Phylogenetic reconstructions revealed that the new FS genomes belongs to the ECSA genotype and falls within a single strongly supported monophyletic clade that includes other older CHIKV sequences from the same location, suggesting the persistence of the virus during distinct epidemic seasons. We also performed minor variants analysis and found a small number of SNPs per sample (b_29L and e_45SR = 16 SNPs, c_29SR = 29 and d_45PL and f_45FL = 21 SNPs). Out of the 93 SNPs found, 71 are synonymous, 21 are non-synonymous and one generated a stop codon. Although those mutations are not related to the increase of virus replication and/or infectivity, some SNPs were found in non-structural proteins which may have an effect on viral evasion from the mammal immunological system. These findings reinforce the needing of further studies on those variants and of continued genomic surveillance strategies to track viral adaptations and to monitor CHIKV epidemics for improved public health control.
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spelling pubmed-69487412020-01-17 Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil Goes de Jesus, Jaqueline da Luz Wallau, Gabriel Lima Maia, Maricelia Xavier, Joilson Oliveira Lima, Maria Aparecida Fonseca, Vagner Salgado de Abreu, Alvaro Fraga de Oliveira Tosta, Stephane Ramos do Amaral, Helineide Andrade Barbosa Lima, Italo Viana Silva, Paloma Carlos dos Santos, Daiana Sousa de Oliveira, Aline Campos de Souza, Siane Barreto Falcão, Melissa Cerqueira, Erenilde Ceschini Machado, Laís Sobral, Mariana Carolina Teodoro Rezende, Tatiana Maria Ribeiro Pereira, Mylena Mota Pereira, Felicidade Pereira Gusmão Maia, Zuinara Freitas de Oliveira França, Rafael Luiz de Abreu, André Campelo de Albuquerque e Melo, Carlos Frederico Rodrigues Faria, Nuno Venâncio da Cunha, Rivaldo Giovanetti, Marta Alcantara, Luiz Carlos Junior PLoS One Research Article The chikungunya East/Central/South/Africa virus lineage (CHIKV-ECSA) was first detected in Brazil in the municipality of Feira de Santana (FS) by mid 2014. Following that, a large number of CHIKV cases have been notified in FS, which is the second-most populous city in Bahia state, northeastern Brazil, and plays an important role on the spread to other Brazilian states due to climate conditions and the abundance of competent vectors. To better understand CHIKV dynamics in Bahia state, we generated 5 complete genome sequences from a local outbreak raised in Serraria Brasil, a neighbourhood in FS, by next-generation sequencing using Illumina approach. Phylogenetic reconstructions revealed that the new FS genomes belongs to the ECSA genotype and falls within a single strongly supported monophyletic clade that includes other older CHIKV sequences from the same location, suggesting the persistence of the virus during distinct epidemic seasons. We also performed minor variants analysis and found a small number of SNPs per sample (b_29L and e_45SR = 16 SNPs, c_29SR = 29 and d_45PL and f_45FL = 21 SNPs). Out of the 93 SNPs found, 71 are synonymous, 21 are non-synonymous and one generated a stop codon. Although those mutations are not related to the increase of virus replication and/or infectivity, some SNPs were found in non-structural proteins which may have an effect on viral evasion from the mammal immunological system. These findings reinforce the needing of further studies on those variants and of continued genomic surveillance strategies to track viral adaptations and to monitor CHIKV epidemics for improved public health control. Public Library of Science 2020-01-08 /pmc/articles/PMC6948741/ /pubmed/31914137 http://dx.doi.org/10.1371/journal.pone.0226098 Text en © 2020 Goes de Jesus et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Goes de Jesus, Jaqueline
da Luz Wallau, Gabriel
Lima Maia, Maricelia
Xavier, Joilson
Oliveira Lima, Maria Aparecida
Fonseca, Vagner
Salgado de Abreu, Alvaro
Fraga de Oliveira Tosta, Stephane
Ramos do Amaral, Helineide
Andrade Barbosa Lima, Italo
Viana Silva, Paloma
Carlos dos Santos, Daiana
Sousa de Oliveira, Aline
Campos de Souza, Siane
Barreto Falcão, Melissa
Cerqueira, Erenilde
Ceschini Machado, Laís
Sobral, Mariana Carolina
Teodoro Rezende, Tatiana Maria
Ribeiro Pereira, Mylena
Mota Pereira, Felicidade
Pereira Gusmão Maia, Zuinara
Freitas de Oliveira França, Rafael
Luiz de Abreu, André
Campelo de Albuquerque e Melo, Carlos Frederico
Rodrigues Faria, Nuno
Venâncio da Cunha, Rivaldo
Giovanetti, Marta
Alcantara, Luiz Carlos Junior
Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil
title Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil
title_full Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil
title_fullStr Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil
title_full_unstemmed Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil
title_short Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil
title_sort persistence of chikungunya ecsa genotype and local outbreak in an upper medium class neighborhood in northeast brazil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948741/
https://www.ncbi.nlm.nih.gov/pubmed/31914137
http://dx.doi.org/10.1371/journal.pone.0226098
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