Cargando…
Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil
The chikungunya East/Central/South/Africa virus lineage (CHIKV-ECSA) was first detected in Brazil in the municipality of Feira de Santana (FS) by mid 2014. Following that, a large number of CHIKV cases have been notified in FS, which is the second-most populous city in Bahia state, northeastern Braz...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948741/ https://www.ncbi.nlm.nih.gov/pubmed/31914137 http://dx.doi.org/10.1371/journal.pone.0226098 |
_version_ | 1783485804562087936 |
---|---|
author | Goes de Jesus, Jaqueline da Luz Wallau, Gabriel Lima Maia, Maricelia Xavier, Joilson Oliveira Lima, Maria Aparecida Fonseca, Vagner Salgado de Abreu, Alvaro Fraga de Oliveira Tosta, Stephane Ramos do Amaral, Helineide Andrade Barbosa Lima, Italo Viana Silva, Paloma Carlos dos Santos, Daiana Sousa de Oliveira, Aline Campos de Souza, Siane Barreto Falcão, Melissa Cerqueira, Erenilde Ceschini Machado, Laís Sobral, Mariana Carolina Teodoro Rezende, Tatiana Maria Ribeiro Pereira, Mylena Mota Pereira, Felicidade Pereira Gusmão Maia, Zuinara Freitas de Oliveira França, Rafael Luiz de Abreu, André Campelo de Albuquerque e Melo, Carlos Frederico Rodrigues Faria, Nuno Venâncio da Cunha, Rivaldo Giovanetti, Marta Alcantara, Luiz Carlos Junior |
author_facet | Goes de Jesus, Jaqueline da Luz Wallau, Gabriel Lima Maia, Maricelia Xavier, Joilson Oliveira Lima, Maria Aparecida Fonseca, Vagner Salgado de Abreu, Alvaro Fraga de Oliveira Tosta, Stephane Ramos do Amaral, Helineide Andrade Barbosa Lima, Italo Viana Silva, Paloma Carlos dos Santos, Daiana Sousa de Oliveira, Aline Campos de Souza, Siane Barreto Falcão, Melissa Cerqueira, Erenilde Ceschini Machado, Laís Sobral, Mariana Carolina Teodoro Rezende, Tatiana Maria Ribeiro Pereira, Mylena Mota Pereira, Felicidade Pereira Gusmão Maia, Zuinara Freitas de Oliveira França, Rafael Luiz de Abreu, André Campelo de Albuquerque e Melo, Carlos Frederico Rodrigues Faria, Nuno Venâncio da Cunha, Rivaldo Giovanetti, Marta Alcantara, Luiz Carlos Junior |
author_sort | Goes de Jesus, Jaqueline |
collection | PubMed |
description | The chikungunya East/Central/South/Africa virus lineage (CHIKV-ECSA) was first detected in Brazil in the municipality of Feira de Santana (FS) by mid 2014. Following that, a large number of CHIKV cases have been notified in FS, which is the second-most populous city in Bahia state, northeastern Brazil, and plays an important role on the spread to other Brazilian states due to climate conditions and the abundance of competent vectors. To better understand CHIKV dynamics in Bahia state, we generated 5 complete genome sequences from a local outbreak raised in Serraria Brasil, a neighbourhood in FS, by next-generation sequencing using Illumina approach. Phylogenetic reconstructions revealed that the new FS genomes belongs to the ECSA genotype and falls within a single strongly supported monophyletic clade that includes other older CHIKV sequences from the same location, suggesting the persistence of the virus during distinct epidemic seasons. We also performed minor variants analysis and found a small number of SNPs per sample (b_29L and e_45SR = 16 SNPs, c_29SR = 29 and d_45PL and f_45FL = 21 SNPs). Out of the 93 SNPs found, 71 are synonymous, 21 are non-synonymous and one generated a stop codon. Although those mutations are not related to the increase of virus replication and/or infectivity, some SNPs were found in non-structural proteins which may have an effect on viral evasion from the mammal immunological system. These findings reinforce the needing of further studies on those variants and of continued genomic surveillance strategies to track viral adaptations and to monitor CHIKV epidemics for improved public health control. |
format | Online Article Text |
id | pubmed-6948741 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-69487412020-01-17 Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil Goes de Jesus, Jaqueline da Luz Wallau, Gabriel Lima Maia, Maricelia Xavier, Joilson Oliveira Lima, Maria Aparecida Fonseca, Vagner Salgado de Abreu, Alvaro Fraga de Oliveira Tosta, Stephane Ramos do Amaral, Helineide Andrade Barbosa Lima, Italo Viana Silva, Paloma Carlos dos Santos, Daiana Sousa de Oliveira, Aline Campos de Souza, Siane Barreto Falcão, Melissa Cerqueira, Erenilde Ceschini Machado, Laís Sobral, Mariana Carolina Teodoro Rezende, Tatiana Maria Ribeiro Pereira, Mylena Mota Pereira, Felicidade Pereira Gusmão Maia, Zuinara Freitas de Oliveira França, Rafael Luiz de Abreu, André Campelo de Albuquerque e Melo, Carlos Frederico Rodrigues Faria, Nuno Venâncio da Cunha, Rivaldo Giovanetti, Marta Alcantara, Luiz Carlos Junior PLoS One Research Article The chikungunya East/Central/South/Africa virus lineage (CHIKV-ECSA) was first detected in Brazil in the municipality of Feira de Santana (FS) by mid 2014. Following that, a large number of CHIKV cases have been notified in FS, which is the second-most populous city in Bahia state, northeastern Brazil, and plays an important role on the spread to other Brazilian states due to climate conditions and the abundance of competent vectors. To better understand CHIKV dynamics in Bahia state, we generated 5 complete genome sequences from a local outbreak raised in Serraria Brasil, a neighbourhood in FS, by next-generation sequencing using Illumina approach. Phylogenetic reconstructions revealed that the new FS genomes belongs to the ECSA genotype and falls within a single strongly supported monophyletic clade that includes other older CHIKV sequences from the same location, suggesting the persistence of the virus during distinct epidemic seasons. We also performed minor variants analysis and found a small number of SNPs per sample (b_29L and e_45SR = 16 SNPs, c_29SR = 29 and d_45PL and f_45FL = 21 SNPs). Out of the 93 SNPs found, 71 are synonymous, 21 are non-synonymous and one generated a stop codon. Although those mutations are not related to the increase of virus replication and/or infectivity, some SNPs were found in non-structural proteins which may have an effect on viral evasion from the mammal immunological system. These findings reinforce the needing of further studies on those variants and of continued genomic surveillance strategies to track viral adaptations and to monitor CHIKV epidemics for improved public health control. Public Library of Science 2020-01-08 /pmc/articles/PMC6948741/ /pubmed/31914137 http://dx.doi.org/10.1371/journal.pone.0226098 Text en © 2020 Goes de Jesus et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Goes de Jesus, Jaqueline da Luz Wallau, Gabriel Lima Maia, Maricelia Xavier, Joilson Oliveira Lima, Maria Aparecida Fonseca, Vagner Salgado de Abreu, Alvaro Fraga de Oliveira Tosta, Stephane Ramos do Amaral, Helineide Andrade Barbosa Lima, Italo Viana Silva, Paloma Carlos dos Santos, Daiana Sousa de Oliveira, Aline Campos de Souza, Siane Barreto Falcão, Melissa Cerqueira, Erenilde Ceschini Machado, Laís Sobral, Mariana Carolina Teodoro Rezende, Tatiana Maria Ribeiro Pereira, Mylena Mota Pereira, Felicidade Pereira Gusmão Maia, Zuinara Freitas de Oliveira França, Rafael Luiz de Abreu, André Campelo de Albuquerque e Melo, Carlos Frederico Rodrigues Faria, Nuno Venâncio da Cunha, Rivaldo Giovanetti, Marta Alcantara, Luiz Carlos Junior Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil |
title | Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil |
title_full | Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil |
title_fullStr | Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil |
title_full_unstemmed | Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil |
title_short | Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil |
title_sort | persistence of chikungunya ecsa genotype and local outbreak in an upper medium class neighborhood in northeast brazil |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948741/ https://www.ncbi.nlm.nih.gov/pubmed/31914137 http://dx.doi.org/10.1371/journal.pone.0226098 |
work_keys_str_mv | AT goesdejesusjaqueline persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT daluzwallaugabriel persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT limamaiamaricelia persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT xavierjoilson persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT oliveiralimamariaaparecida persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT fonsecavagner persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT salgadodeabreualvaro persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT fragadeoliveiratostastephane persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT ramosdoamaralhelineide persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT andradebarbosalimaitalo persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT vianasilvapaloma persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT carlosdossantosdaiana persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT sousadeoliveiraaline persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT camposdesouzasiane persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT barretofalcaomelissa persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT cerqueiraerenilde persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT ceschinimachadolais persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT sobralmarianacarolina persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT teodororezendetatianamaria persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT ribeiropereiramylena persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT motapereirafelicidade persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT pereiragusmaomaiazuinara persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT freitasdeoliveirafrancarafael persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT luizdeabreuandre persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT campelodealbuquerqueemelocarlosfrederico persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT rodriguesfarianuno persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT venanciodacunharivaldo persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT giovanettimarta persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil AT alcantaraluizcarlosjunior persistenceofchikungunyaecsagenotypeandlocaloutbreakinanuppermediumclassneighborhoodinnortheastbrazil |