Cargando…
Study on JV Virus in Patients with Colon Cancer Type Adenocarcinoma
INTRODUCTION: Colorectal cancer is the most repetitious malignancies with high mortality worldwide. JC virus (JCV) is ubiquitous Polyomavirus, with seroprevalence rates ranging from 70% to 90% in adult population. Recently the role of JCV have been reported in many malignant tumors worldwide. The as...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
West Asia Organization for Cancer Prevention
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948910/ https://www.ncbi.nlm.nih.gov/pubmed/31030488 http://dx.doi.org/10.31557/APJCP.2019.20.4.1147 |
_version_ | 1783485823514050560 |
---|---|
author | Haghi Navand, Azadeh Teimoori, Ali Makvandi, Manoochehr Nisi, Nilofar Seyedian, Seyed Saeid Ranjbari, Nastarn Ahmadi Angali, Kambiz Keyani, Hadis Tabasi, Maryam Pourjabari, Keyvan |
author_facet | Haghi Navand, Azadeh Teimoori, Ali Makvandi, Manoochehr Nisi, Nilofar Seyedian, Seyed Saeid Ranjbari, Nastarn Ahmadi Angali, Kambiz Keyani, Hadis Tabasi, Maryam Pourjabari, Keyvan |
author_sort | Haghi Navand, Azadeh |
collection | PubMed |
description | INTRODUCTION: Colorectal cancer is the most repetitious malignancies with high mortality worldwide. JC virus (JCV) is ubiquitous Polyomavirus, with seroprevalence rates ranging from 70% to 90% in adult population. Recently the role of JCV have been reported in many malignant tumors worldwide. The association of JCV was reported in patients with colon and rectum cancers. Thus this study was conducted to evaluate the association of JCV DNA in patients with colon cancer type Adenocarcinoma. MATERIAL AND METHODS: A total of 120 formalin-fixed paraffin-embedded tissue blocks samples were collected including 20/40(50%) males, 20/40(50%) females patients with Colorectal Cancer(CRC), and 80 (50% males, 50% females) patients with benign tumor as a control. DNA was extracted for all the samples. Nested PCR was carried out for detection of Vp1/T-Ag junction genome in JCV genome by Nested-PCR assay. Randomly, PCR products of 6 samples were sequenced to analysis the partial JCV DNA. The phylogeny tree was constructed to determine homology identity with other JCV. RESULTS: 4/40(10%) samples of test group and 10/80 (12.5%) of control samples were positive for JCV DNA (P= 0.69). Out of 4 samples positive for JC DNA, 3(7.5%) were males and 1(2.4%) female (P=0.29). The frequency of JCV DNA in age group> 50 years was 4/32(10%), while in age group <50 years was 0/8 (0%) (p= 0.29). CONCLUSION: prevalence of JCV DNA was among 10% patients with CRC and 12.5% benign tumors (p=0.69). The distribution of JCV DNA was among 7.5% male and 2.5% female (p= 0.29). The frequency of JCV DNA was among 10% cases of age group >50 years and 0% of age group <50 years (P= 0.29). The subsequent T-Ag protein expression might explain the increased risk of colorectal cancer and requires further investigation. |
format | Online Article Text |
id | pubmed-6948910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | West Asia Organization for Cancer Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-69489102020-02-04 Study on JV Virus in Patients with Colon Cancer Type Adenocarcinoma Haghi Navand, Azadeh Teimoori, Ali Makvandi, Manoochehr Nisi, Nilofar Seyedian, Seyed Saeid Ranjbari, Nastarn Ahmadi Angali, Kambiz Keyani, Hadis Tabasi, Maryam Pourjabari, Keyvan Asian Pac J Cancer Prev Research Article INTRODUCTION: Colorectal cancer is the most repetitious malignancies with high mortality worldwide. JC virus (JCV) is ubiquitous Polyomavirus, with seroprevalence rates ranging from 70% to 90% in adult population. Recently the role of JCV have been reported in many malignant tumors worldwide. The association of JCV was reported in patients with colon and rectum cancers. Thus this study was conducted to evaluate the association of JCV DNA in patients with colon cancer type Adenocarcinoma. MATERIAL AND METHODS: A total of 120 formalin-fixed paraffin-embedded tissue blocks samples were collected including 20/40(50%) males, 20/40(50%) females patients with Colorectal Cancer(CRC), and 80 (50% males, 50% females) patients with benign tumor as a control. DNA was extracted for all the samples. Nested PCR was carried out for detection of Vp1/T-Ag junction genome in JCV genome by Nested-PCR assay. Randomly, PCR products of 6 samples were sequenced to analysis the partial JCV DNA. The phylogeny tree was constructed to determine homology identity with other JCV. RESULTS: 4/40(10%) samples of test group and 10/80 (12.5%) of control samples were positive for JCV DNA (P= 0.69). Out of 4 samples positive for JC DNA, 3(7.5%) were males and 1(2.4%) female (P=0.29). The frequency of JCV DNA in age group> 50 years was 4/32(10%), while in age group <50 years was 0/8 (0%) (p= 0.29). CONCLUSION: prevalence of JCV DNA was among 10% patients with CRC and 12.5% benign tumors (p=0.69). The distribution of JCV DNA was among 7.5% male and 2.5% female (p= 0.29). The frequency of JCV DNA was among 10% cases of age group >50 years and 0% of age group <50 years (P= 0.29). The subsequent T-Ag protein expression might explain the increased risk of colorectal cancer and requires further investigation. West Asia Organization for Cancer Prevention 2019 /pmc/articles/PMC6948910/ /pubmed/31030488 http://dx.doi.org/10.31557/APJCP.2019.20.4.1147 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Haghi Navand, Azadeh Teimoori, Ali Makvandi, Manoochehr Nisi, Nilofar Seyedian, Seyed Saeid Ranjbari, Nastarn Ahmadi Angali, Kambiz Keyani, Hadis Tabasi, Maryam Pourjabari, Keyvan Study on JV Virus in Patients with Colon Cancer Type Adenocarcinoma |
title | Study on JV Virus in Patients with Colon Cancer Type Adenocarcinoma |
title_full | Study on JV Virus in Patients with Colon Cancer Type Adenocarcinoma |
title_fullStr | Study on JV Virus in Patients with Colon Cancer Type Adenocarcinoma |
title_full_unstemmed | Study on JV Virus in Patients with Colon Cancer Type Adenocarcinoma |
title_short | Study on JV Virus in Patients with Colon Cancer Type Adenocarcinoma |
title_sort | study on jv virus in patients with colon cancer type adenocarcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948910/ https://www.ncbi.nlm.nih.gov/pubmed/31030488 http://dx.doi.org/10.31557/APJCP.2019.20.4.1147 |
work_keys_str_mv | AT haghinavandazadeh studyonjvvirusinpatientswithcoloncancertypeadenocarcinoma AT teimooriali studyonjvvirusinpatientswithcoloncancertypeadenocarcinoma AT makvandimanoochehr studyonjvvirusinpatientswithcoloncancertypeadenocarcinoma AT nisinilofar studyonjvvirusinpatientswithcoloncancertypeadenocarcinoma AT seyedianseyedsaeid studyonjvvirusinpatientswithcoloncancertypeadenocarcinoma AT ranjbarinastarn studyonjvvirusinpatientswithcoloncancertypeadenocarcinoma AT ahmadiangalikambiz studyonjvvirusinpatientswithcoloncancertypeadenocarcinoma AT keyanihadis studyonjvvirusinpatientswithcoloncancertypeadenocarcinoma AT tabasimaryam studyonjvvirusinpatientswithcoloncancertypeadenocarcinoma AT pourjabarikeyvan studyonjvvirusinpatientswithcoloncancertypeadenocarcinoma |