Cargando…

Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis

Methyltransferase-like 1 (METTL1) mediated 7-methylguanosine (m7G) is crucial for the regulation of chemoresistance in cancer treatment. However, the role of METTL1 in regulating chemoresistance of colon cancer (CC) cells to cisplatin is still unclear. This study established the cisplatin-resistant...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yang, Yang, Chunyan, Zhao, Yong, Chi, Qiang, Wang, Zhen, Sun, Boshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949057/
https://www.ncbi.nlm.nih.gov/pubmed/31866582
http://dx.doi.org/10.18632/aging.102575
_version_ 1783485841531731968
author Liu, Yang
Yang, Chunyan
Zhao, Yong
Chi, Qiang
Wang, Zhen
Sun, Boshi
author_facet Liu, Yang
Yang, Chunyan
Zhao, Yong
Chi, Qiang
Wang, Zhen
Sun, Boshi
author_sort Liu, Yang
collection PubMed
description Methyltransferase-like 1 (METTL1) mediated 7-methylguanosine (m7G) is crucial for the regulation of chemoresistance in cancer treatment. However, the role of METTL1 in regulating chemoresistance of colon cancer (CC) cells to cisplatin is still unclear. This study established the cisplatin-resistant CC (CR-CC) cells and found that METTL1 was low-expressed in CR-CC cells compared to their paired cisplatin-sensitive CC (CS-CC) cells. Besides, overexpressed METTL1 enhanced the cytotoxic effects of cisplatin on CR-CC cells. In addition, miR-149-3p was the downstream target of METTL1, which could be positively regulated by METTL1. Further results validated that miR-149-3p was low-expressed in CR-CC cells comparing to the CS-CC cells. In addition, the promoting effects of overexpressed METTL1 on cisplatin induced CR-CC cell death were abrogated by synergistically knocking down miR-149-3p. Furthermore, S100A4/p53 axis was the downstream target of METTL1 and miR-149-3p, and either overexpressed METTL1 or miR-149-3p increased p53 protein levels in CR-CC cells, which were reversed by upregulating S100A4. Similarly, the promoting effects of overexpressed METTL1 on cisplatin-induced CR-CC cell death were abrogated by overexpressing S100A4. Taken together, overexpression of METTL1 sensitized CR-CC cells to cisplatin by modulating miR-149-3p/S100A4/p53 axis.
format Online
Article
Text
id pubmed-6949057
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Impact Journals
record_format MEDLINE/PubMed
spelling pubmed-69490572020-01-13 Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis Liu, Yang Yang, Chunyan Zhao, Yong Chi, Qiang Wang, Zhen Sun, Boshi Aging (Albany NY) Research Paper Methyltransferase-like 1 (METTL1) mediated 7-methylguanosine (m7G) is crucial for the regulation of chemoresistance in cancer treatment. However, the role of METTL1 in regulating chemoresistance of colon cancer (CC) cells to cisplatin is still unclear. This study established the cisplatin-resistant CC (CR-CC) cells and found that METTL1 was low-expressed in CR-CC cells compared to their paired cisplatin-sensitive CC (CS-CC) cells. Besides, overexpressed METTL1 enhanced the cytotoxic effects of cisplatin on CR-CC cells. In addition, miR-149-3p was the downstream target of METTL1, which could be positively regulated by METTL1. Further results validated that miR-149-3p was low-expressed in CR-CC cells comparing to the CS-CC cells. In addition, the promoting effects of overexpressed METTL1 on cisplatin induced CR-CC cell death were abrogated by synergistically knocking down miR-149-3p. Furthermore, S100A4/p53 axis was the downstream target of METTL1 and miR-149-3p, and either overexpressed METTL1 or miR-149-3p increased p53 protein levels in CR-CC cells, which were reversed by upregulating S100A4. Similarly, the promoting effects of overexpressed METTL1 on cisplatin-induced CR-CC cell death were abrogated by overexpressing S100A4. Taken together, overexpression of METTL1 sensitized CR-CC cells to cisplatin by modulating miR-149-3p/S100A4/p53 axis. Impact Journals 2019-12-20 /pmc/articles/PMC6949057/ /pubmed/31866582 http://dx.doi.org/10.18632/aging.102575 Text en Copyright © 2019 Liu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Liu, Yang
Yang, Chunyan
Zhao, Yong
Chi, Qiang
Wang, Zhen
Sun, Boshi
Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis
title Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis
title_full Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis
title_fullStr Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis
title_full_unstemmed Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis
title_short Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis
title_sort overexpressed methyltransferase-like 1 (mettl1) increased chemosensitivity of colon cancer cells to cisplatin by regulating mir-149-3p/s100a4/p53 axis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949057/
https://www.ncbi.nlm.nih.gov/pubmed/31866582
http://dx.doi.org/10.18632/aging.102575
work_keys_str_mv AT liuyang overexpressedmethyltransferaselike1mettl1increasedchemosensitivityofcoloncancercellstocisplatinbyregulatingmir1493ps100a4p53axis
AT yangchunyan overexpressedmethyltransferaselike1mettl1increasedchemosensitivityofcoloncancercellstocisplatinbyregulatingmir1493ps100a4p53axis
AT zhaoyong overexpressedmethyltransferaselike1mettl1increasedchemosensitivityofcoloncancercellstocisplatinbyregulatingmir1493ps100a4p53axis
AT chiqiang overexpressedmethyltransferaselike1mettl1increasedchemosensitivityofcoloncancercellstocisplatinbyregulatingmir1493ps100a4p53axis
AT wangzhen overexpressedmethyltransferaselike1mettl1increasedchemosensitivityofcoloncancercellstocisplatinbyregulatingmir1493ps100a4p53axis
AT sunboshi overexpressedmethyltransferaselike1mettl1increasedchemosensitivityofcoloncancercellstocisplatinbyregulatingmir1493ps100a4p53axis