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CircRNA AFF4 promotes osteoblast cells proliferation and inhibits apoptosis via the Mir-7223-5p/PIK3R1 axis

Fracture healing is a complex process involving various cell types, cytokines, and mRNAs. Here, we report the roles of the circRNA AFF4/miR-7223-5p/PIK3R1 axis during fracture healing. We found that increased expression of PIK3R1 during fracture healing is directly associated with augmented prolifer...

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Autores principales: Mi, Bobin, Xiong, Yuan, Chen, Lang, Yan, Chenchen, Endo, Yori, Liu, Yi, Liu, Jing, Hu, Liangcong, Hu, Yiqiang, Sun, Yun, Cao, Faqi, Zhou, Wu, Liu, Guohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949079/
https://www.ncbi.nlm.nih.gov/pubmed/31848327
http://dx.doi.org/10.18632/aging.102524
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author Mi, Bobin
Xiong, Yuan
Chen, Lang
Yan, Chenchen
Endo, Yori
Liu, Yi
Liu, Jing
Hu, Liangcong
Hu, Yiqiang
Sun, Yun
Cao, Faqi
Zhou, Wu
Liu, Guohui
author_facet Mi, Bobin
Xiong, Yuan
Chen, Lang
Yan, Chenchen
Endo, Yori
Liu, Yi
Liu, Jing
Hu, Liangcong
Hu, Yiqiang
Sun, Yun
Cao, Faqi
Zhou, Wu
Liu, Guohui
author_sort Mi, Bobin
collection PubMed
description Fracture healing is a complex process involving various cell types, cytokines, and mRNAs. Here, we report the roles of the circRNA AFF4/miR-7223-5p/PIK3R1 axis during fracture healing. We found that increased expression of PIK3R1 during fracture healing is directly associated with augmented proliferation and decreased apoptosis of MC3T3-E1 cells. Furthermore, miR-7223-5p targeted PI3KR1 and inhibited MC3T3-E1 proliferation while promoting apoptosis. CircRNA AFF4 acted as a sponge of miR-7223-5p, thereby promoting MC3T3-E1 cell proliferation and inhibiting apoptosis. Local injection of circRNA AFF4 into femoral fracture sites promoted fracture healing in vivo while the injection of miR-7223-5p delayed healing. These findings suggest that CircRNA AFF4 promotes fracture healing by targeting the miR-7223-5p/PIK3R1 axis, and suggests miR-7223-5p, CircRNA AFF4, and the miR-7223-5p/PIK3R1 axis are potential therapeutic targets for improving fracture healing.
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spelling pubmed-69490792020-01-13 CircRNA AFF4 promotes osteoblast cells proliferation and inhibits apoptosis via the Mir-7223-5p/PIK3R1 axis Mi, Bobin Xiong, Yuan Chen, Lang Yan, Chenchen Endo, Yori Liu, Yi Liu, Jing Hu, Liangcong Hu, Yiqiang Sun, Yun Cao, Faqi Zhou, Wu Liu, Guohui Aging (Albany NY) Research Paper Fracture healing is a complex process involving various cell types, cytokines, and mRNAs. Here, we report the roles of the circRNA AFF4/miR-7223-5p/PIK3R1 axis during fracture healing. We found that increased expression of PIK3R1 during fracture healing is directly associated with augmented proliferation and decreased apoptosis of MC3T3-E1 cells. Furthermore, miR-7223-5p targeted PI3KR1 and inhibited MC3T3-E1 proliferation while promoting apoptosis. CircRNA AFF4 acted as a sponge of miR-7223-5p, thereby promoting MC3T3-E1 cell proliferation and inhibiting apoptosis. Local injection of circRNA AFF4 into femoral fracture sites promoted fracture healing in vivo while the injection of miR-7223-5p delayed healing. These findings suggest that CircRNA AFF4 promotes fracture healing by targeting the miR-7223-5p/PIK3R1 axis, and suggests miR-7223-5p, CircRNA AFF4, and the miR-7223-5p/PIK3R1 axis are potential therapeutic targets for improving fracture healing. Impact Journals 2019-12-17 /pmc/articles/PMC6949079/ /pubmed/31848327 http://dx.doi.org/10.18632/aging.102524 Text en Copyright © 2019 Mi et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Mi, Bobin
Xiong, Yuan
Chen, Lang
Yan, Chenchen
Endo, Yori
Liu, Yi
Liu, Jing
Hu, Liangcong
Hu, Yiqiang
Sun, Yun
Cao, Faqi
Zhou, Wu
Liu, Guohui
CircRNA AFF4 promotes osteoblast cells proliferation and inhibits apoptosis via the Mir-7223-5p/PIK3R1 axis
title CircRNA AFF4 promotes osteoblast cells proliferation and inhibits apoptosis via the Mir-7223-5p/PIK3R1 axis
title_full CircRNA AFF4 promotes osteoblast cells proliferation and inhibits apoptosis via the Mir-7223-5p/PIK3R1 axis
title_fullStr CircRNA AFF4 promotes osteoblast cells proliferation and inhibits apoptosis via the Mir-7223-5p/PIK3R1 axis
title_full_unstemmed CircRNA AFF4 promotes osteoblast cells proliferation and inhibits apoptosis via the Mir-7223-5p/PIK3R1 axis
title_short CircRNA AFF4 promotes osteoblast cells proliferation and inhibits apoptosis via the Mir-7223-5p/PIK3R1 axis
title_sort circrna aff4 promotes osteoblast cells proliferation and inhibits apoptosis via the mir-7223-5p/pik3r1 axis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949079/
https://www.ncbi.nlm.nih.gov/pubmed/31848327
http://dx.doi.org/10.18632/aging.102524
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