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Functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in Aspergillus niger
Galactofuranose (Galf)-containing glycostructures are important to secure the integrity of the fungal cell wall. Golgi-localized Galf-transferases (Gfs) have been identified in Aspergillus nidulans and Aspergillus fumigatus. BLASTp searches identified three putative Galf-transferases in Aspergillus...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949202/ https://www.ncbi.nlm.nih.gov/pubmed/31372664 http://dx.doi.org/10.1007/s00203-019-01709-w |
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author | Arentshorst, Mark de Lange, Davina Park, Joohae Lagendijk, Ellen L. Alazi, Ebru van den Hondel, Cees A. M. J. J. Ram, Arthur F. J. |
author_facet | Arentshorst, Mark de Lange, Davina Park, Joohae Lagendijk, Ellen L. Alazi, Ebru van den Hondel, Cees A. M. J. J. Ram, Arthur F. J. |
author_sort | Arentshorst, Mark |
collection | PubMed |
description | Galactofuranose (Galf)-containing glycostructures are important to secure the integrity of the fungal cell wall. Golgi-localized Galf-transferases (Gfs) have been identified in Aspergillus nidulans and Aspergillus fumigatus. BLASTp searches identified three putative Galf-transferases in Aspergillus niger. Phylogenetic analysis showed that they group in three distinct groups. Characterization of the three Galf-transferases in A. niger by constructing single, double, and triple mutants revealed that gfsA is most important for Galf biosynthesis. The growth phenotypes of the ΔgfsA mutant are less severe than that of the ΔgfsAC mutant, indicating that GfsA and GfsC have redundant functions. Deletion of gfsB did not result in any growth defect and combining ΔgfsB with other deletion mutants did not exacerbate the growth phenotype. RT-qPCR experiments showed that induction of the agsA gene was higher in the ΔgfsAC and ΔgfsABC compared to the single mutants, indicating a severe cell wall stress response after multiple gfs gene deletions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00203-019-01709-w) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6949202 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-69492022020-01-23 Functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in Aspergillus niger Arentshorst, Mark de Lange, Davina Park, Joohae Lagendijk, Ellen L. Alazi, Ebru van den Hondel, Cees A. M. J. J. Ram, Arthur F. J. Arch Microbiol Short Communication Galactofuranose (Galf)-containing glycostructures are important to secure the integrity of the fungal cell wall. Golgi-localized Galf-transferases (Gfs) have been identified in Aspergillus nidulans and Aspergillus fumigatus. BLASTp searches identified three putative Galf-transferases in Aspergillus niger. Phylogenetic analysis showed that they group in three distinct groups. Characterization of the three Galf-transferases in A. niger by constructing single, double, and triple mutants revealed that gfsA is most important for Galf biosynthesis. The growth phenotypes of the ΔgfsA mutant are less severe than that of the ΔgfsAC mutant, indicating that GfsA and GfsC have redundant functions. Deletion of gfsB did not result in any growth defect and combining ΔgfsB with other deletion mutants did not exacerbate the growth phenotype. RT-qPCR experiments showed that induction of the agsA gene was higher in the ΔgfsAC and ΔgfsABC compared to the single mutants, indicating a severe cell wall stress response after multiple gfs gene deletions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00203-019-01709-w) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-08-01 2020 /pmc/articles/PMC6949202/ /pubmed/31372664 http://dx.doi.org/10.1007/s00203-019-01709-w Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Short Communication Arentshorst, Mark de Lange, Davina Park, Joohae Lagendijk, Ellen L. Alazi, Ebru van den Hondel, Cees A. M. J. J. Ram, Arthur F. J. Functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in Aspergillus niger |
title | Functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in Aspergillus niger |
title_full | Functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in Aspergillus niger |
title_fullStr | Functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in Aspergillus niger |
title_full_unstemmed | Functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in Aspergillus niger |
title_short | Functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in Aspergillus niger |
title_sort | functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in aspergillus niger |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949202/ https://www.ncbi.nlm.nih.gov/pubmed/31372664 http://dx.doi.org/10.1007/s00203-019-01709-w |
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