Cargando…
Comparison of three different serum-free light-chain assays—implications on diagnostic and therapeutic monitoring of multiple myeloma
The measurement of serum-free light chains (FLC) is standard of care in the diagnosis and management of multiple myeloma (MM). The revised international myeloma working group (IMWG) implemented the involved FLC/noninvolved FLC (iFLC/niFLC) ratio as a biomarker for MM requiring treatment. Recently, a...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949235/ https://www.ncbi.nlm.nih.gov/pubmed/31915365 http://dx.doi.org/10.1038/s41408-019-0267-8 |
_version_ | 1783485878841114624 |
---|---|
author | Schieferdecker, Aneta Hörber, Sebastian Ums, Monika Besemer, Britta Bokemeyer, Carsten Peter, Andreas Weisel, Katja |
author_facet | Schieferdecker, Aneta Hörber, Sebastian Ums, Monika Besemer, Britta Bokemeyer, Carsten Peter, Andreas Weisel, Katja |
author_sort | Schieferdecker, Aneta |
collection | PubMed |
description | The measurement of serum-free light chains (FLC) is standard of care in the diagnosis and management of multiple myeloma (MM). The revised international myeloma working group (IMWG) implemented the involved FLC/noninvolved FLC (iFLC/niFLC) ratio as a biomarker for MM requiring treatment. Recently, a new definition of high-risk smoldering MM (SMM) including iFLC/niFLC ratio was published. These recommendations were solely based on a single assay method (Freelite assay). Today, two additional assays, N Latex FLC and ELISA-based Sebia FLC, are available. Here, we report on a single-center-study comparing results of all three different assays for FLC correlation and its potential implications for diagnostic and clinical use. In total, 187 samples from 47 MM patients were examined, and determination of FLC was performed. Comparison analyses showed similar FLC results for Sebia FLC and N Latex FLC assay with markedly lower absolute values for κ/λ ratio compared with Freelite. Values of λ FLC exhibited high variability. The ratio of iFLC/niFLC showed significant discrepancies among these assays. Our data demonstrate that the three available assays may result in markedly discrepant results, and should not be used interchangeably to monitor patients. Furthermore, modifications of the assay-specific diagnostic (iFLC/niFLC) thresholds for SMM and MM are recommended. |
format | Online Article Text |
id | pubmed-6949235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69492352020-01-13 Comparison of three different serum-free light-chain assays—implications on diagnostic and therapeutic monitoring of multiple myeloma Schieferdecker, Aneta Hörber, Sebastian Ums, Monika Besemer, Britta Bokemeyer, Carsten Peter, Andreas Weisel, Katja Blood Cancer J Article The measurement of serum-free light chains (FLC) is standard of care in the diagnosis and management of multiple myeloma (MM). The revised international myeloma working group (IMWG) implemented the involved FLC/noninvolved FLC (iFLC/niFLC) ratio as a biomarker for MM requiring treatment. Recently, a new definition of high-risk smoldering MM (SMM) including iFLC/niFLC ratio was published. These recommendations were solely based on a single assay method (Freelite assay). Today, two additional assays, N Latex FLC and ELISA-based Sebia FLC, are available. Here, we report on a single-center-study comparing results of all three different assays for FLC correlation and its potential implications for diagnostic and clinical use. In total, 187 samples from 47 MM patients were examined, and determination of FLC was performed. Comparison analyses showed similar FLC results for Sebia FLC and N Latex FLC assay with markedly lower absolute values for κ/λ ratio compared with Freelite. Values of λ FLC exhibited high variability. The ratio of iFLC/niFLC showed significant discrepancies among these assays. Our data demonstrate that the three available assays may result in markedly discrepant results, and should not be used interchangeably to monitor patients. Furthermore, modifications of the assay-specific diagnostic (iFLC/niFLC) thresholds for SMM and MM are recommended. Nature Publishing Group UK 2020-01-09 /pmc/articles/PMC6949235/ /pubmed/31915365 http://dx.doi.org/10.1038/s41408-019-0267-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Schieferdecker, Aneta Hörber, Sebastian Ums, Monika Besemer, Britta Bokemeyer, Carsten Peter, Andreas Weisel, Katja Comparison of three different serum-free light-chain assays—implications on diagnostic and therapeutic monitoring of multiple myeloma |
title | Comparison of three different serum-free light-chain assays—implications on diagnostic and therapeutic monitoring of multiple myeloma |
title_full | Comparison of three different serum-free light-chain assays—implications on diagnostic and therapeutic monitoring of multiple myeloma |
title_fullStr | Comparison of three different serum-free light-chain assays—implications on diagnostic and therapeutic monitoring of multiple myeloma |
title_full_unstemmed | Comparison of three different serum-free light-chain assays—implications on diagnostic and therapeutic monitoring of multiple myeloma |
title_short | Comparison of three different serum-free light-chain assays—implications on diagnostic and therapeutic monitoring of multiple myeloma |
title_sort | comparison of three different serum-free light-chain assays—implications on diagnostic and therapeutic monitoring of multiple myeloma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949235/ https://www.ncbi.nlm.nih.gov/pubmed/31915365 http://dx.doi.org/10.1038/s41408-019-0267-8 |
work_keys_str_mv | AT schieferdeckeraneta comparisonofthreedifferentserumfreelightchainassaysimplicationsondiagnosticandtherapeuticmonitoringofmultiplemyeloma AT horbersebastian comparisonofthreedifferentserumfreelightchainassaysimplicationsondiagnosticandtherapeuticmonitoringofmultiplemyeloma AT umsmonika comparisonofthreedifferentserumfreelightchainassaysimplicationsondiagnosticandtherapeuticmonitoringofmultiplemyeloma AT besemerbritta comparisonofthreedifferentserumfreelightchainassaysimplicationsondiagnosticandtherapeuticmonitoringofmultiplemyeloma AT bokemeyercarsten comparisonofthreedifferentserumfreelightchainassaysimplicationsondiagnosticandtherapeuticmonitoringofmultiplemyeloma AT peterandreas comparisonofthreedifferentserumfreelightchainassaysimplicationsondiagnosticandtherapeuticmonitoringofmultiplemyeloma AT weiselkatja comparisonofthreedifferentserumfreelightchainassaysimplicationsondiagnosticandtherapeuticmonitoringofmultiplemyeloma |