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Visualization of mucosal field in HPV positive and negative oropharyngeal squamous cell carcinomas: combined genomic and radiology based 3D model
The aim of this study was to visualize the tumor propagation and surrounding mucosal field in radiography-based 3D model for advanced stage HNSCC and combine it with HPV genotyping and miRNA expression characterization of the visualized area. 25 patients with T1-3 clinical stage HNSCC were enrolled...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949264/ https://www.ncbi.nlm.nih.gov/pubmed/31913295 http://dx.doi.org/10.1038/s41598-019-56429-4 |
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author | Orosz, Eva Gombos, Katalin Petrevszky, Nerina Csonka, David Haber, Istvan Kaszas, Balint Toth, Arnold Molnar, Krisztian Kalacs, Krisztina Piski, Zalan Gerlinger, Imre Burian, Andras Bellyei, Szabolcs Szanyi, Istvan |
author_facet | Orosz, Eva Gombos, Katalin Petrevszky, Nerina Csonka, David Haber, Istvan Kaszas, Balint Toth, Arnold Molnar, Krisztian Kalacs, Krisztina Piski, Zalan Gerlinger, Imre Burian, Andras Bellyei, Szabolcs Szanyi, Istvan |
author_sort | Orosz, Eva |
collection | PubMed |
description | The aim of this study was to visualize the tumor propagation and surrounding mucosal field in radiography-based 3D model for advanced stage HNSCC and combine it with HPV genotyping and miRNA expression characterization of the visualized area. 25 patients with T1-3 clinical stage HNSCC were enrolled in mapping biopsy sampling. Biopsy samples were evaluated for HPV positivity and miR-21-5p, miR-143, miR-155, miR-221-5p expression in Digital Droplet PCR system. Significant miRNA expression differences of HPV positive tumor tissue biopsies were found for miR-21-5p, miR-143 and miR-221-5p compared to the HPV negative tumor biopsy series. Peritumoral mucosa showed patchy pattern alterations of miR-21-5p and miR-155 in HPV positive cases, while gradual change of miR-21-5p and miR-221-5p was seen in HPV negative tumors. In our study we found differences of the miRNA expression patterns among the HPV positive and negative tumorous tissues as well as the surrounding mucosal fields. The CT based 3D models of the cancer field and surrounding mucosal surface can be utilized to improve proper preoperative planning. Complex evaluation of HNSCC tissue organization field can elucidate the clinical and molecular differentiation of HPV positive and negative cases, and enhance effective organ saving therapeutic strategies. |
format | Online Article Text |
id | pubmed-6949264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69492642020-01-13 Visualization of mucosal field in HPV positive and negative oropharyngeal squamous cell carcinomas: combined genomic and radiology based 3D model Orosz, Eva Gombos, Katalin Petrevszky, Nerina Csonka, David Haber, Istvan Kaszas, Balint Toth, Arnold Molnar, Krisztian Kalacs, Krisztina Piski, Zalan Gerlinger, Imre Burian, Andras Bellyei, Szabolcs Szanyi, Istvan Sci Rep Article The aim of this study was to visualize the tumor propagation and surrounding mucosal field in radiography-based 3D model for advanced stage HNSCC and combine it with HPV genotyping and miRNA expression characterization of the visualized area. 25 patients with T1-3 clinical stage HNSCC were enrolled in mapping biopsy sampling. Biopsy samples were evaluated for HPV positivity and miR-21-5p, miR-143, miR-155, miR-221-5p expression in Digital Droplet PCR system. Significant miRNA expression differences of HPV positive tumor tissue biopsies were found for miR-21-5p, miR-143 and miR-221-5p compared to the HPV negative tumor biopsy series. Peritumoral mucosa showed patchy pattern alterations of miR-21-5p and miR-155 in HPV positive cases, while gradual change of miR-21-5p and miR-221-5p was seen in HPV negative tumors. In our study we found differences of the miRNA expression patterns among the HPV positive and negative tumorous tissues as well as the surrounding mucosal fields. The CT based 3D models of the cancer field and surrounding mucosal surface can be utilized to improve proper preoperative planning. Complex evaluation of HNSCC tissue organization field can elucidate the clinical and molecular differentiation of HPV positive and negative cases, and enhance effective organ saving therapeutic strategies. Nature Publishing Group UK 2020-01-08 /pmc/articles/PMC6949264/ /pubmed/31913295 http://dx.doi.org/10.1038/s41598-019-56429-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Orosz, Eva Gombos, Katalin Petrevszky, Nerina Csonka, David Haber, Istvan Kaszas, Balint Toth, Arnold Molnar, Krisztian Kalacs, Krisztina Piski, Zalan Gerlinger, Imre Burian, Andras Bellyei, Szabolcs Szanyi, Istvan Visualization of mucosal field in HPV positive and negative oropharyngeal squamous cell carcinomas: combined genomic and radiology based 3D model |
title | Visualization of mucosal field in HPV positive and negative oropharyngeal squamous cell carcinomas: combined genomic and radiology based 3D model |
title_full | Visualization of mucosal field in HPV positive and negative oropharyngeal squamous cell carcinomas: combined genomic and radiology based 3D model |
title_fullStr | Visualization of mucosal field in HPV positive and negative oropharyngeal squamous cell carcinomas: combined genomic and radiology based 3D model |
title_full_unstemmed | Visualization of mucosal field in HPV positive and negative oropharyngeal squamous cell carcinomas: combined genomic and radiology based 3D model |
title_short | Visualization of mucosal field in HPV positive and negative oropharyngeal squamous cell carcinomas: combined genomic and radiology based 3D model |
title_sort | visualization of mucosal field in hpv positive and negative oropharyngeal squamous cell carcinomas: combined genomic and radiology based 3d model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949264/ https://www.ncbi.nlm.nih.gov/pubmed/31913295 http://dx.doi.org/10.1038/s41598-019-56429-4 |
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