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Characterization of goat prions demonstrates geographical variation of scrapie strains in Europe and reveals the composite nature of prion strains
Bovine Spongiform Encephalopathy (BSE) is the only animal prion which has been recognized as a zoonotic agent so far. The identification of BSE in two goats raised the need to reliably identify BSE in small ruminants. However, our understanding of scrapie strain diversity in small ruminants remains...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949283/ https://www.ncbi.nlm.nih.gov/pubmed/31913327 http://dx.doi.org/10.1038/s41598-019-57005-6 |
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author | Nonno, Romolo Marin-Moreno, Alba Carlos Espinosa, Juan Fast, Christine Van Keulen, Lucien Spiropoulos, John Lantier, Isabelle Andreoletti, Olivier Pirisinu, Laura Di Bari, Michele A. Aguilar-Calvo, Patricia Sklaviadis, Theodoros Papasavva-Stylianou, Penelope Acutis, Pier Luigi Acin, Cristina Bossers, Alex Jacobs, Jorge G. Vaccari, Gabriele D’Agostino, Claudia Chiappini, Barbara Lantier, Frederic Groschup, Martin H. Agrimi, Umberto Maria Torres, Juan Langeveld, Jan P. M. |
author_facet | Nonno, Romolo Marin-Moreno, Alba Carlos Espinosa, Juan Fast, Christine Van Keulen, Lucien Spiropoulos, John Lantier, Isabelle Andreoletti, Olivier Pirisinu, Laura Di Bari, Michele A. Aguilar-Calvo, Patricia Sklaviadis, Theodoros Papasavva-Stylianou, Penelope Acutis, Pier Luigi Acin, Cristina Bossers, Alex Jacobs, Jorge G. Vaccari, Gabriele D’Agostino, Claudia Chiappini, Barbara Lantier, Frederic Groschup, Martin H. Agrimi, Umberto Maria Torres, Juan Langeveld, Jan P. M. |
author_sort | Nonno, Romolo |
collection | PubMed |
description | Bovine Spongiform Encephalopathy (BSE) is the only animal prion which has been recognized as a zoonotic agent so far. The identification of BSE in two goats raised the need to reliably identify BSE in small ruminants. However, our understanding of scrapie strain diversity in small ruminants remains ill-defined, thus limiting the accuracy of BSE surveillance and spreading fear that BSE might lurk unrecognized in goats. We investigated prion strain diversity in a large panel of European goats by a novel experimental approach that, instead of assessing the neuropathological profile after serial transmissions in a single animal model, was based on the direct interaction of prion isolates with several recipient rodent models expressing small ruminants or heterologous prion proteins. The findings show that the biological properties of scrapie isolates display different patterns of geographical distribution in Europe and suggest that goat BSE could be reliably discriminated from a wide range of biologically and geographically diverse goat prion isolates. Finally, most field prion isolates showed composite strain features, with discrete strain components or sub-strains being present in different proportions in individual goats or tissues. This has important implications for understanding the nature and evolution of scrapie strains and their transmissibility to other species, including humans. |
format | Online Article Text |
id | pubmed-6949283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69492832020-01-13 Characterization of goat prions demonstrates geographical variation of scrapie strains in Europe and reveals the composite nature of prion strains Nonno, Romolo Marin-Moreno, Alba Carlos Espinosa, Juan Fast, Christine Van Keulen, Lucien Spiropoulos, John Lantier, Isabelle Andreoletti, Olivier Pirisinu, Laura Di Bari, Michele A. Aguilar-Calvo, Patricia Sklaviadis, Theodoros Papasavva-Stylianou, Penelope Acutis, Pier Luigi Acin, Cristina Bossers, Alex Jacobs, Jorge G. Vaccari, Gabriele D’Agostino, Claudia Chiappini, Barbara Lantier, Frederic Groschup, Martin H. Agrimi, Umberto Maria Torres, Juan Langeveld, Jan P. M. Sci Rep Article Bovine Spongiform Encephalopathy (BSE) is the only animal prion which has been recognized as a zoonotic agent so far. The identification of BSE in two goats raised the need to reliably identify BSE in small ruminants. However, our understanding of scrapie strain diversity in small ruminants remains ill-defined, thus limiting the accuracy of BSE surveillance and spreading fear that BSE might lurk unrecognized in goats. We investigated prion strain diversity in a large panel of European goats by a novel experimental approach that, instead of assessing the neuropathological profile after serial transmissions in a single animal model, was based on the direct interaction of prion isolates with several recipient rodent models expressing small ruminants or heterologous prion proteins. The findings show that the biological properties of scrapie isolates display different patterns of geographical distribution in Europe and suggest that goat BSE could be reliably discriminated from a wide range of biologically and geographically diverse goat prion isolates. Finally, most field prion isolates showed composite strain features, with discrete strain components or sub-strains being present in different proportions in individual goats or tissues. This has important implications for understanding the nature and evolution of scrapie strains and their transmissibility to other species, including humans. Nature Publishing Group UK 2020-01-08 /pmc/articles/PMC6949283/ /pubmed/31913327 http://dx.doi.org/10.1038/s41598-019-57005-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Nonno, Romolo Marin-Moreno, Alba Carlos Espinosa, Juan Fast, Christine Van Keulen, Lucien Spiropoulos, John Lantier, Isabelle Andreoletti, Olivier Pirisinu, Laura Di Bari, Michele A. Aguilar-Calvo, Patricia Sklaviadis, Theodoros Papasavva-Stylianou, Penelope Acutis, Pier Luigi Acin, Cristina Bossers, Alex Jacobs, Jorge G. Vaccari, Gabriele D’Agostino, Claudia Chiappini, Barbara Lantier, Frederic Groschup, Martin H. Agrimi, Umberto Maria Torres, Juan Langeveld, Jan P. M. Characterization of goat prions demonstrates geographical variation of scrapie strains in Europe and reveals the composite nature of prion strains |
title | Characterization of goat prions demonstrates geographical variation of scrapie strains in Europe and reveals the composite nature of prion strains |
title_full | Characterization of goat prions demonstrates geographical variation of scrapie strains in Europe and reveals the composite nature of prion strains |
title_fullStr | Characterization of goat prions demonstrates geographical variation of scrapie strains in Europe and reveals the composite nature of prion strains |
title_full_unstemmed | Characterization of goat prions demonstrates geographical variation of scrapie strains in Europe and reveals the composite nature of prion strains |
title_short | Characterization of goat prions demonstrates geographical variation of scrapie strains in Europe and reveals the composite nature of prion strains |
title_sort | characterization of goat prions demonstrates geographical variation of scrapie strains in europe and reveals the composite nature of prion strains |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949283/ https://www.ncbi.nlm.nih.gov/pubmed/31913327 http://dx.doi.org/10.1038/s41598-019-57005-6 |
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