Cargando…

Synergistic Inhibition by Bacteriocin and Bacteriophage against Staphylococcus aureus

Staphylococcus aureus is a representative pathogenic bacterium carefully controlled in the dairy industry because it causes bovine mastitis and thus, can enter the dairy chain. Furthermore, the emergence of multi-drug resistant S. aureus is a big problem. We previously isolated a Lactococcus lactis...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Seon-Gyu, Lee, Young-Duck, Park, Jong-Hyun, Moon, Gi-Seong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Food Science of Animal Resources 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949521/
https://www.ncbi.nlm.nih.gov/pubmed/31950117
http://dx.doi.org/10.5851/kosfa.2019.e95
_version_ 1783485923289202688
author Kim, Seon-Gyu
Lee, Young-Duck
Park, Jong-Hyun
Moon, Gi-Seong
author_facet Kim, Seon-Gyu
Lee, Young-Duck
Park, Jong-Hyun
Moon, Gi-Seong
author_sort Kim, Seon-Gyu
collection PubMed
description Staphylococcus aureus is a representative pathogenic bacterium carefully controlled in the dairy industry because it causes bovine mastitis and thus, can enter the dairy chain. Furthermore, the emergence of multi-drug resistant S. aureus is a big problem. We previously isolated a Lactococcus lactis strain producing a bacteriocin that exhibited strong antimicrobial activity against S. aureus. In this study, we investigated the synergistic inhibition of S. aureus by the bacteriocin and a bacteriophage (SAP84) which is specific to the organism. The bacteriocin (12.5–100 AU/mL) inhibited the growth of S. aureus KCTC 3881 in a dose-dependent manner, as did the bacteriophage SAP84 (0.001–1 MOI; multiplicity of infection). Co-treatment with the bacteriocin (100 AU/mL) and the bacteriophage (0.1 MOI) significantly inhibited the growth of S. aureus compared to each treatment alone (bacteriocin or bacteriophage), indicating the two components showed synergistic inhibition of S. aureus. Therefore, the bacteriocin and bacteriophage combination can be used as a good strategy for controlling pathogenic bacteria.
format Online
Article
Text
id pubmed-6949521
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Korean Society for Food Science of Animal Resources
record_format MEDLINE/PubMed
spelling pubmed-69495212020-01-16 Synergistic Inhibition by Bacteriocin and Bacteriophage against Staphylococcus aureus Kim, Seon-Gyu Lee, Young-Duck Park, Jong-Hyun Moon, Gi-Seong Food Sci Anim Resour Short Communication Staphylococcus aureus is a representative pathogenic bacterium carefully controlled in the dairy industry because it causes bovine mastitis and thus, can enter the dairy chain. Furthermore, the emergence of multi-drug resistant S. aureus is a big problem. We previously isolated a Lactococcus lactis strain producing a bacteriocin that exhibited strong antimicrobial activity against S. aureus. In this study, we investigated the synergistic inhibition of S. aureus by the bacteriocin and a bacteriophage (SAP84) which is specific to the organism. The bacteriocin (12.5–100 AU/mL) inhibited the growth of S. aureus KCTC 3881 in a dose-dependent manner, as did the bacteriophage SAP84 (0.001–1 MOI; multiplicity of infection). Co-treatment with the bacteriocin (100 AU/mL) and the bacteriophage (0.1 MOI) significantly inhibited the growth of S. aureus compared to each treatment alone (bacteriocin or bacteriophage), indicating the two components showed synergistic inhibition of S. aureus. Therefore, the bacteriocin and bacteriophage combination can be used as a good strategy for controlling pathogenic bacteria. Korean Society for Food Science of Animal Resources 2019-12 2019-12-31 /pmc/articles/PMC6949521/ /pubmed/31950117 http://dx.doi.org/10.5851/kosfa.2019.e95 Text en © Korean Society for Food Science of Animal Resources http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Kim, Seon-Gyu
Lee, Young-Duck
Park, Jong-Hyun
Moon, Gi-Seong
Synergistic Inhibition by Bacteriocin and Bacteriophage against Staphylococcus aureus
title Synergistic Inhibition by Bacteriocin and Bacteriophage against Staphylococcus aureus
title_full Synergistic Inhibition by Bacteriocin and Bacteriophage against Staphylococcus aureus
title_fullStr Synergistic Inhibition by Bacteriocin and Bacteriophage against Staphylococcus aureus
title_full_unstemmed Synergistic Inhibition by Bacteriocin and Bacteriophage against Staphylococcus aureus
title_short Synergistic Inhibition by Bacteriocin and Bacteriophage against Staphylococcus aureus
title_sort synergistic inhibition by bacteriocin and bacteriophage against staphylococcus aureus
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949521/
https://www.ncbi.nlm.nih.gov/pubmed/31950117
http://dx.doi.org/10.5851/kosfa.2019.e95
work_keys_str_mv AT kimseongyu synergisticinhibitionbybacteriocinandbacteriophageagainststaphylococcusaureus
AT leeyoungduck synergisticinhibitionbybacteriocinandbacteriophageagainststaphylococcusaureus
AT parkjonghyun synergisticinhibitionbybacteriocinandbacteriophageagainststaphylococcusaureus
AT moongiseong synergisticinhibitionbybacteriocinandbacteriophageagainststaphylococcusaureus