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A fixed-target platform for serial femtosecond crystallography in a hydrated environment

For serial femtosecond crystallography at X-ray free-electron lasers, which entails collection of single-pulse diffraction patterns from a constantly refreshed supply of microcrystalline sample, delivery of the sample into the X-ray beam path while maintaining low background remains a technical chal...

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Detalles Bibliográficos
Autores principales: Shelby, M. L., Gilbile, D., Grant, T. D., Seuring, C., Segelke, B. W., He, W., Evans, A. C., Pakendorf, T., Fischer, P., Hunter, M. S., Batyuk, A., Barthelmess, M., Meents, A., Coleman, M. A., Kuhl, T. L., Frank, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949605/
https://www.ncbi.nlm.nih.gov/pubmed/31949902
http://dx.doi.org/10.1107/S2052252519014003
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author Shelby, M. L.
Gilbile, D.
Grant, T. D.
Seuring, C.
Segelke, B. W.
He, W.
Evans, A. C.
Pakendorf, T.
Fischer, P.
Hunter, M. S.
Batyuk, A.
Barthelmess, M.
Meents, A.
Coleman, M. A.
Kuhl, T. L.
Frank, M.
author_facet Shelby, M. L.
Gilbile, D.
Grant, T. D.
Seuring, C.
Segelke, B. W.
He, W.
Evans, A. C.
Pakendorf, T.
Fischer, P.
Hunter, M. S.
Batyuk, A.
Barthelmess, M.
Meents, A.
Coleman, M. A.
Kuhl, T. L.
Frank, M.
author_sort Shelby, M. L.
collection PubMed
description For serial femtosecond crystallography at X-ray free-electron lasers, which entails collection of single-pulse diffraction patterns from a constantly refreshed supply of microcrystalline sample, delivery of the sample into the X-ray beam path while maintaining low background remains a technical challenge for some experiments, especially where this methodology is applied to relatively low-ordered samples or those difficult to purify and crystallize in large quantities. This work demonstrates a scheme to encapsulate biological samples using polymer thin films and graphene to maintain sample hydration in vacuum conditions. The encapsulated sample is delivered into the X-ray beam on fixed targets for rapid scanning using the Roadrunner fixed-target system towards a long-term goal of low-background measurements on weakly diffracting samples. As a proof of principle, we used microcrystals of the 24 kDa rapid encystment protein (REP24) to provide a benchmark for polymer/graphene sandwich performance. The REP24 microcrystal unit cell obtained from our sandwiched in-vacuum sample was consistent with previously established unit-cell parameters and with those measured by us without encapsulation in humidified helium, indicating that the platform is robust against evaporative losses. While significant scattering from water was observed because of the sample-deposition method, the polymer/graphene sandwich itself was shown to contribute minimally to background scattering.
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spelling pubmed-69496052020-01-16 A fixed-target platform for serial femtosecond crystallography in a hydrated environment Shelby, M. L. Gilbile, D. Grant, T. D. Seuring, C. Segelke, B. W. He, W. Evans, A. C. Pakendorf, T. Fischer, P. Hunter, M. S. Batyuk, A. Barthelmess, M. Meents, A. Coleman, M. A. Kuhl, T. L. Frank, M. IUCrJ Research Papers For serial femtosecond crystallography at X-ray free-electron lasers, which entails collection of single-pulse diffraction patterns from a constantly refreshed supply of microcrystalline sample, delivery of the sample into the X-ray beam path while maintaining low background remains a technical challenge for some experiments, especially where this methodology is applied to relatively low-ordered samples or those difficult to purify and crystallize in large quantities. This work demonstrates a scheme to encapsulate biological samples using polymer thin films and graphene to maintain sample hydration in vacuum conditions. The encapsulated sample is delivered into the X-ray beam on fixed targets for rapid scanning using the Roadrunner fixed-target system towards a long-term goal of low-background measurements on weakly diffracting samples. As a proof of principle, we used microcrystals of the 24 kDa rapid encystment protein (REP24) to provide a benchmark for polymer/graphene sandwich performance. The REP24 microcrystal unit cell obtained from our sandwiched in-vacuum sample was consistent with previously established unit-cell parameters and with those measured by us without encapsulation in humidified helium, indicating that the platform is robust against evaporative losses. While significant scattering from water was observed because of the sample-deposition method, the polymer/graphene sandwich itself was shown to contribute minimally to background scattering. International Union of Crystallography 2020-01-01 /pmc/articles/PMC6949605/ /pubmed/31949902 http://dx.doi.org/10.1107/S2052252519014003 Text en © Shelby et al. 2020 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Papers
Shelby, M. L.
Gilbile, D.
Grant, T. D.
Seuring, C.
Segelke, B. W.
He, W.
Evans, A. C.
Pakendorf, T.
Fischer, P.
Hunter, M. S.
Batyuk, A.
Barthelmess, M.
Meents, A.
Coleman, M. A.
Kuhl, T. L.
Frank, M.
A fixed-target platform for serial femtosecond crystallography in a hydrated environment
title A fixed-target platform for serial femtosecond crystallography in a hydrated environment
title_full A fixed-target platform for serial femtosecond crystallography in a hydrated environment
title_fullStr A fixed-target platform for serial femtosecond crystallography in a hydrated environment
title_full_unstemmed A fixed-target platform for serial femtosecond crystallography in a hydrated environment
title_short A fixed-target platform for serial femtosecond crystallography in a hydrated environment
title_sort fixed-target platform for serial femtosecond crystallography in a hydrated environment
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949605/
https://www.ncbi.nlm.nih.gov/pubmed/31949902
http://dx.doi.org/10.1107/S2052252519014003
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