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Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway

The aim of this study was to investigate the effects of deoxynivalenol (DON) exposure on the inflammatory injury nuclear factor kappa-B (NF-κB) pathway in intestinal epithelial cells (IPEC-J2 cells) of pig. The different concentrations of DON (0, 125, 250, 500, 1000, 2000 ng/mL) were added to the cu...

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Autores principales: Wang, Xichun, Zhang, Yafei, Zhao, Jie, Cao, Li, Zhu, Lei, Huang, Yingying, Chen, Xiaofang, Rahman, Sajid Ur, Feng, Shibin, Li, Yu, Wu, Jinjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950076/
https://www.ncbi.nlm.nih.gov/pubmed/31888297
http://dx.doi.org/10.3390/toxins11120733
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author Wang, Xichun
Zhang, Yafei
Zhao, Jie
Cao, Li
Zhu, Lei
Huang, Yingying
Chen, Xiaofang
Rahman, Sajid Ur
Feng, Shibin
Li, Yu
Wu, Jinjie
author_facet Wang, Xichun
Zhang, Yafei
Zhao, Jie
Cao, Li
Zhu, Lei
Huang, Yingying
Chen, Xiaofang
Rahman, Sajid Ur
Feng, Shibin
Li, Yu
Wu, Jinjie
author_sort Wang, Xichun
collection PubMed
description The aim of this study was to investigate the effects of deoxynivalenol (DON) exposure on the inflammatory injury nuclear factor kappa-B (NF-κB) pathway in intestinal epithelial cells (IPEC-J2 cells) of pig. The different concentrations of DON (0, 125, 250, 500, 1000, 2000 ng/mL) were added to the culture solution for treatment. The NF-κB pathway inhibitor pyrrolidine dithiocarbamate (PDTC) was used as a reference. The results showed that when the DON concentration increased, the cell density decreased and seemed damaged. With the increase of DON concentration in the culture medium, the action of diamine oxidase (DAO) in the culture supernatant also increased. The activities of IL-6, TNF-α, and NO in the cells were increased with the increasing DON concentration. The relative mRNA expression of IL-1β and IL-6 were increased in the cells. The mRNA relative expression of NF-κB p65, IKKα, and IKKβ were upregulated with the increasing of DON concentration, while the relative expression of IκB-α mRNA was downregulated. At the same time, the expression of NF-κB p65 protein increased gradually in the cytoplasm and nucleus with a higher concentration of DON. These results showed that DON could change the morphology of IPEC-J2 cells, destroy its submicroscopic structure, and enhance the permeability of cell membrane, as well as upregulate the transcription of some inflammatory factors and change the expression of NF-κB-related gene or protein in cells.
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spelling pubmed-69500762020-01-13 Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway Wang, Xichun Zhang, Yafei Zhao, Jie Cao, Li Zhu, Lei Huang, Yingying Chen, Xiaofang Rahman, Sajid Ur Feng, Shibin Li, Yu Wu, Jinjie Toxins (Basel) Article The aim of this study was to investigate the effects of deoxynivalenol (DON) exposure on the inflammatory injury nuclear factor kappa-B (NF-κB) pathway in intestinal epithelial cells (IPEC-J2 cells) of pig. The different concentrations of DON (0, 125, 250, 500, 1000, 2000 ng/mL) were added to the culture solution for treatment. The NF-κB pathway inhibitor pyrrolidine dithiocarbamate (PDTC) was used as a reference. The results showed that when the DON concentration increased, the cell density decreased and seemed damaged. With the increase of DON concentration in the culture medium, the action of diamine oxidase (DAO) in the culture supernatant also increased. The activities of IL-6, TNF-α, and NO in the cells were increased with the increasing DON concentration. The relative mRNA expression of IL-1β and IL-6 were increased in the cells. The mRNA relative expression of NF-κB p65, IKKα, and IKKβ were upregulated with the increasing of DON concentration, while the relative expression of IκB-α mRNA was downregulated. At the same time, the expression of NF-κB p65 protein increased gradually in the cytoplasm and nucleus with a higher concentration of DON. These results showed that DON could change the morphology of IPEC-J2 cells, destroy its submicroscopic structure, and enhance the permeability of cell membrane, as well as upregulate the transcription of some inflammatory factors and change the expression of NF-κB-related gene or protein in cells. MDPI 2019-12-16 /pmc/articles/PMC6950076/ /pubmed/31888297 http://dx.doi.org/10.3390/toxins11120733 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Xichun
Zhang, Yafei
Zhao, Jie
Cao, Li
Zhu, Lei
Huang, Yingying
Chen, Xiaofang
Rahman, Sajid Ur
Feng, Shibin
Li, Yu
Wu, Jinjie
Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway
title Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway
title_full Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway
title_fullStr Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway
title_full_unstemmed Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway
title_short Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway
title_sort deoxynivalenol induces inflammatory injury in ipec-j2 cells via nf-κb signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950076/
https://www.ncbi.nlm.nih.gov/pubmed/31888297
http://dx.doi.org/10.3390/toxins11120733
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