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Pediatric Infections by Human mastadenovirus C Types 2, 89, and a Recombinant Type Detected in Japan between 2011 and 2018

Between 2011 and 2018, 518 respiratory adenovirus infections were diagnosed in a pediatric clinic in Shizuoka, Japan. Detection and typing were performed by partial sequencing of both hexon- and fiber-coding regions which identified: adenovirus type 1 (Ad-1, n = 85), Ad-2 (n = 160), Ad-3 (n = 193),...

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Autores principales: Takahashi, Kenichiro, Gonzalez, Gabriel, Kobayashi, Masaaki, Hanaoka, Nozomu, Carr, Michael J., Konagaya, Masami, Nojiri, Naomi, Ogi, Miki, Fujimoto, Tsuguto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950085/
https://www.ncbi.nlm.nih.gov/pubmed/31817764
http://dx.doi.org/10.3390/v11121131
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author Takahashi, Kenichiro
Gonzalez, Gabriel
Kobayashi, Masaaki
Hanaoka, Nozomu
Carr, Michael J.
Konagaya, Masami
Nojiri, Naomi
Ogi, Miki
Fujimoto, Tsuguto
author_facet Takahashi, Kenichiro
Gonzalez, Gabriel
Kobayashi, Masaaki
Hanaoka, Nozomu
Carr, Michael J.
Konagaya, Masami
Nojiri, Naomi
Ogi, Miki
Fujimoto, Tsuguto
author_sort Takahashi, Kenichiro
collection PubMed
description Between 2011 and 2018, 518 respiratory adenovirus infections were diagnosed in a pediatric clinic in Shizuoka, Japan. Detection and typing were performed by partial sequencing of both hexon- and fiber-coding regions which identified: adenovirus type 1 (Ad-1, n = 85), Ad-2 (n = 160), Ad-3 (n = 193), Ad-4 (n = 18), Ad-5 (n = 27), Ad-11 (n = 2), Ad-54 (n = 3), and Ad-56 (n = 1). Considering previous reports of the circulation of an endemic recombinant Ad-2, e.g., Ad-89, 100 samples typed as Ad-2 were randomly selected for further molecular typing by sequencing the penton base-coding region. Despite the high nucleotide sequence conservation in the penton base- coding region, 27 samples showed 98% identity to Ad-2. Furthermore, 14 samples showed 97.7% identity to Ad-2 and 99.8% identity to Ad-89, while the remaining 13 samples showed an average 98% pairwise identity to other Ad-C types and clustered with Ad-5. The samples typed as Ad-89 (n = 14) and as a recombinant Ad type (P5H2F2) (n = 13) represented 27% of cases originally diagnosed as Ad-2, and were detected sporadically. Therefore, two previously uncharacterized types in Japan, Ad-89 and a recombinant Ad-C, were shown to circulate in children. This study creates a precedent to evaluate the epidemiology and divergence among Ad-C types by comprehensively considering the type classification of adenoviruses.
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spelling pubmed-69500852020-01-13 Pediatric Infections by Human mastadenovirus C Types 2, 89, and a Recombinant Type Detected in Japan between 2011 and 2018 Takahashi, Kenichiro Gonzalez, Gabriel Kobayashi, Masaaki Hanaoka, Nozomu Carr, Michael J. Konagaya, Masami Nojiri, Naomi Ogi, Miki Fujimoto, Tsuguto Viruses Article Between 2011 and 2018, 518 respiratory adenovirus infections were diagnosed in a pediatric clinic in Shizuoka, Japan. Detection and typing were performed by partial sequencing of both hexon- and fiber-coding regions which identified: adenovirus type 1 (Ad-1, n = 85), Ad-2 (n = 160), Ad-3 (n = 193), Ad-4 (n = 18), Ad-5 (n = 27), Ad-11 (n = 2), Ad-54 (n = 3), and Ad-56 (n = 1). Considering previous reports of the circulation of an endemic recombinant Ad-2, e.g., Ad-89, 100 samples typed as Ad-2 were randomly selected for further molecular typing by sequencing the penton base-coding region. Despite the high nucleotide sequence conservation in the penton base- coding region, 27 samples showed 98% identity to Ad-2. Furthermore, 14 samples showed 97.7% identity to Ad-2 and 99.8% identity to Ad-89, while the remaining 13 samples showed an average 98% pairwise identity to other Ad-C types and clustered with Ad-5. The samples typed as Ad-89 (n = 14) and as a recombinant Ad type (P5H2F2) (n = 13) represented 27% of cases originally diagnosed as Ad-2, and were detected sporadically. Therefore, two previously uncharacterized types in Japan, Ad-89 and a recombinant Ad-C, were shown to circulate in children. This study creates a precedent to evaluate the epidemiology and divergence among Ad-C types by comprehensively considering the type classification of adenoviruses. MDPI 2019-12-06 /pmc/articles/PMC6950085/ /pubmed/31817764 http://dx.doi.org/10.3390/v11121131 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Takahashi, Kenichiro
Gonzalez, Gabriel
Kobayashi, Masaaki
Hanaoka, Nozomu
Carr, Michael J.
Konagaya, Masami
Nojiri, Naomi
Ogi, Miki
Fujimoto, Tsuguto
Pediatric Infections by Human mastadenovirus C Types 2, 89, and a Recombinant Type Detected in Japan between 2011 and 2018
title Pediatric Infections by Human mastadenovirus C Types 2, 89, and a Recombinant Type Detected in Japan between 2011 and 2018
title_full Pediatric Infections by Human mastadenovirus C Types 2, 89, and a Recombinant Type Detected in Japan between 2011 and 2018
title_fullStr Pediatric Infections by Human mastadenovirus C Types 2, 89, and a Recombinant Type Detected in Japan between 2011 and 2018
title_full_unstemmed Pediatric Infections by Human mastadenovirus C Types 2, 89, and a Recombinant Type Detected in Japan between 2011 and 2018
title_short Pediatric Infections by Human mastadenovirus C Types 2, 89, and a Recombinant Type Detected in Japan between 2011 and 2018
title_sort pediatric infections by human mastadenovirus c types 2, 89, and a recombinant type detected in japan between 2011 and 2018
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950085/
https://www.ncbi.nlm.nih.gov/pubmed/31817764
http://dx.doi.org/10.3390/v11121131
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