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Serum Proteomic Analysis Reveals Vitamin D-Binding Protein (VDBP) as a Potential Biomarker for Low Bone Mineral Density in Mexican Postmenopausal Women

Osteoporosis is a skeletal disease mainly affecting women over 50 years old and it represents a serious public health problem because of the high socioeconomic burden. This disease is characterized by deterioration of bone microarchitecture, low bone mineral density (BMD), and increased risk of frag...

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Autores principales: Martínez-Aguilar, Mayeli M., Aparicio-Bautista, Diana I., Ramírez-Salazar, Eric G., Reyes-Grajeda, Juan P., De la Cruz-Montoya, Aldo H., Antuna-Puente, Bárbara, Hidalgo-Bravo, Alberto, Rivera-Paredez, Berenice, Ramírez-Palacios, Paula, Quiterio, Manuel, Valdés-Flores, Margarita, Salmerón, Jorge, Velázquez-Cruz, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950314/
https://www.ncbi.nlm.nih.gov/pubmed/31766436
http://dx.doi.org/10.3390/nu11122853
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author Martínez-Aguilar, Mayeli M.
Aparicio-Bautista, Diana I.
Ramírez-Salazar, Eric G.
Reyes-Grajeda, Juan P.
De la Cruz-Montoya, Aldo H.
Antuna-Puente, Bárbara
Hidalgo-Bravo, Alberto
Rivera-Paredez, Berenice
Ramírez-Palacios, Paula
Quiterio, Manuel
Valdés-Flores, Margarita
Salmerón, Jorge
Velázquez-Cruz, Rafael
author_facet Martínez-Aguilar, Mayeli M.
Aparicio-Bautista, Diana I.
Ramírez-Salazar, Eric G.
Reyes-Grajeda, Juan P.
De la Cruz-Montoya, Aldo H.
Antuna-Puente, Bárbara
Hidalgo-Bravo, Alberto
Rivera-Paredez, Berenice
Ramírez-Palacios, Paula
Quiterio, Manuel
Valdés-Flores, Margarita
Salmerón, Jorge
Velázquez-Cruz, Rafael
author_sort Martínez-Aguilar, Mayeli M.
collection PubMed
description Osteoporosis is a skeletal disease mainly affecting women over 50 years old and it represents a serious public health problem because of the high socioeconomic burden. This disease is characterized by deterioration of bone microarchitecture, low bone mineral density (BMD), and increased risk of fragility fractures. This study aimed to identify serum useful proteins as biomarkers for the diagnosis and/or prognosis of osteoporosis and fracture risk. We collected 446 serum samples from postmenopausal women aged ≥45 years old. Based on the BMD measurement, we classified the participants into three groups: osteoporotic, osteopenic, and normal. In an initial discovery stage, we conducted a proteomic approach using two-dimensional differential gel electrophoresis (2D-DIGE). The peptides into the spots of interest were identified through matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF/TOF). Enzyme-linked immunosorbent assay (ELISA) was performed to validate the proteins of interest. We identified 27 spots of interest when comparing low BMD versus normal BMD postmenopausal women. Based on their relevance in bone metabolism, we analyzed three proteins: ceruloplasmin (CP), gelsolin (GSN), and vitamin D-binding protein (VDBP). Our results demonstrated that low serum VDBP levels correlate with low BMD (osteopenic and osteoporotic). Therefore, VDBP could be considered as a novel, potential, and non-invasive biomarker for the early detection of osteoporosis.
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spelling pubmed-69503142020-01-16 Serum Proteomic Analysis Reveals Vitamin D-Binding Protein (VDBP) as a Potential Biomarker for Low Bone Mineral Density in Mexican Postmenopausal Women Martínez-Aguilar, Mayeli M. Aparicio-Bautista, Diana I. Ramírez-Salazar, Eric G. Reyes-Grajeda, Juan P. De la Cruz-Montoya, Aldo H. Antuna-Puente, Bárbara Hidalgo-Bravo, Alberto Rivera-Paredez, Berenice Ramírez-Palacios, Paula Quiterio, Manuel Valdés-Flores, Margarita Salmerón, Jorge Velázquez-Cruz, Rafael Nutrients Article Osteoporosis is a skeletal disease mainly affecting women over 50 years old and it represents a serious public health problem because of the high socioeconomic burden. This disease is characterized by deterioration of bone microarchitecture, low bone mineral density (BMD), and increased risk of fragility fractures. This study aimed to identify serum useful proteins as biomarkers for the diagnosis and/or prognosis of osteoporosis and fracture risk. We collected 446 serum samples from postmenopausal women aged ≥45 years old. Based on the BMD measurement, we classified the participants into three groups: osteoporotic, osteopenic, and normal. In an initial discovery stage, we conducted a proteomic approach using two-dimensional differential gel electrophoresis (2D-DIGE). The peptides into the spots of interest were identified through matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF/TOF). Enzyme-linked immunosorbent assay (ELISA) was performed to validate the proteins of interest. We identified 27 spots of interest when comparing low BMD versus normal BMD postmenopausal women. Based on their relevance in bone metabolism, we analyzed three proteins: ceruloplasmin (CP), gelsolin (GSN), and vitamin D-binding protein (VDBP). Our results demonstrated that low serum VDBP levels correlate with low BMD (osteopenic and osteoporotic). Therefore, VDBP could be considered as a novel, potential, and non-invasive biomarker for the early detection of osteoporosis. MDPI 2019-11-21 /pmc/articles/PMC6950314/ /pubmed/31766436 http://dx.doi.org/10.3390/nu11122853 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Martínez-Aguilar, Mayeli M.
Aparicio-Bautista, Diana I.
Ramírez-Salazar, Eric G.
Reyes-Grajeda, Juan P.
De la Cruz-Montoya, Aldo H.
Antuna-Puente, Bárbara
Hidalgo-Bravo, Alberto
Rivera-Paredez, Berenice
Ramírez-Palacios, Paula
Quiterio, Manuel
Valdés-Flores, Margarita
Salmerón, Jorge
Velázquez-Cruz, Rafael
Serum Proteomic Analysis Reveals Vitamin D-Binding Protein (VDBP) as a Potential Biomarker for Low Bone Mineral Density in Mexican Postmenopausal Women
title Serum Proteomic Analysis Reveals Vitamin D-Binding Protein (VDBP) as a Potential Biomarker for Low Bone Mineral Density in Mexican Postmenopausal Women
title_full Serum Proteomic Analysis Reveals Vitamin D-Binding Protein (VDBP) as a Potential Biomarker for Low Bone Mineral Density in Mexican Postmenopausal Women
title_fullStr Serum Proteomic Analysis Reveals Vitamin D-Binding Protein (VDBP) as a Potential Biomarker for Low Bone Mineral Density in Mexican Postmenopausal Women
title_full_unstemmed Serum Proteomic Analysis Reveals Vitamin D-Binding Protein (VDBP) as a Potential Biomarker for Low Bone Mineral Density in Mexican Postmenopausal Women
title_short Serum Proteomic Analysis Reveals Vitamin D-Binding Protein (VDBP) as a Potential Biomarker for Low Bone Mineral Density in Mexican Postmenopausal Women
title_sort serum proteomic analysis reveals vitamin d-binding protein (vdbp) as a potential biomarker for low bone mineral density in mexican postmenopausal women
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950314/
https://www.ncbi.nlm.nih.gov/pubmed/31766436
http://dx.doi.org/10.3390/nu11122853
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